Mouse Anti-Rat SAPAP (pan) Monoclonal IgG2b, Clone S127-31

  • Catalog number
    SMC-338D-HRP
  • Price
    Please ask
  • Size
    100 µg
  • Clone
    S127-31
  • Immunogen
    Fusion protein amino acids 772-992 (C-terminus) of rat SAPAP1
  • Antibody s full description
    Mouse Anti-Rat SAPAP Monoclonal IgG2b Antibody, Clone: S127-31: HRP
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    DAP 1 ALPHA antibody, DAP 1 antibody, DAP 1 BETA antibody, DAP-1 antibody, DAP-1-ALPHA antibody, DAP-1-BETA antibody, DAP1 antibody, Discs large (Drosophila) homolog associated protein 1 antibody, Discs large homolog associated protein 1 antibody, Disks large associated protein 1 antibody, Disks large associated protein 1 alpha isoform included antibody, Disks large associated protein 1, beta isoform, included antibody, Disks large-associated protein 1 antibody, DLGAP 1 antibody, Dlgap1 antibody, DLGP1_HUMAN antibody GKAP antibody, GKAP/SAPAP antibody, GKPA/SAPAP antibody, Guanylate kinase associated protein antibody, Guanylate kinase-associated protein antibody, hGKAP antibody, KIAA4162 antibody, MGC88156 antibody, mKIAA4162 antibody, PSD 95/SAP90 binding protein 1 antibody, PSD-95/SAP90-binding protein 1 antibody, SAP90/PSD 95 associated protein 1 antibody, SAP90/PSD-95-associated protein 1 antibody SAPAP 1 antibody, SAPAP1 antibody
  • Raised in
    Mouse
  • Antibody s target
    SAPAP
  • Primary research fields
    Neuroscience, Cell Structure, Post-Synaptic Markers
  • Brandname
    None
  • Antibodies applications
    WB, IHC, ICC/IF, IP, AM
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s dilutions
    WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH7.4, 50% glycerol, 0.1% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~120kDa (SAPAP2), ~110kDa (SAPAP1/3/4). It does cross-react with other SAPAPs.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    1 µg/ml of SMC-338 was sufficient for detection of SAPAP 1-4 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cell Membrane , Cell Junction , Synapse , Postsynaptic Cell Membrane , Postsynaptic Density
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    The SAPAP proteins are thought to be adaptor proteins that also interact with different synaptic scaffolding proteins, cytoskeletal and signaling components (1, 2). SAPAP1, 2 and 4 mRNA are targeted to cell bodies, whereas SAPAP3 mRNA is detected mainly in cell bodies (1, 3).
  • Bibliography
    1. Kindler S., et al. (2004) Brain Res. Mol Brain Res. 126: 14-21. 2. Bongiorno-Borbone L., et al. (2005) Biochem, Biophys. Res Commun. 337: 641-646. 3. Welch J.M., Wang D., and Fend G. (2004) J Comp. Neurol. 472: 24-39.
  • Released date
    13-Apr-2010
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    NP_075235.2
  • Gene number
    65040
  • Protein number
    P97836
  • Antibody s datasheet
    Contact our support service
  • Representative figure legend
    Western Blot analysis of Rat brain membrane lysate showing detection of SAPAP protein using Mouse Anti-SAPAP Monoclonal Antibody, Clone S127-31 (SMC-338). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SAPAP Monoclonal Antibody (SMC-338) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-SAPAP Antibody [S127-31] used in Western Blot (WB) on Rat brain membrane lysate (SMC-338)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus, Rattus norvegicus
  • Gene target
    SAPAP   pan   Monoclonal   IgG2b   S127-31  
  • Gene symbol
    SNORD114-31, SNORD115-31, ERVK-31, IGKV3-31, IGLV3-31, RNVU1-31, IGKV3D-31, IL31, OR1D5, RNU6-31P
  • Short name
    Mouse Anti- SAPAP (pan) Monoclonal IgG2b, Clone S127-31
  • Technique
    anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
  • Host
    mouse
  • Isotype
    IgG2b, IgG2b
  • Label
    HRP
  • Species
    Rat, Mouses, Rats
  • Alternative name
    Mouse Antibody toRat SAPAP (pan) monoclonal IgG2b, clonality S127-31
  • Alternative technique
    murine, antibodies
  • Clone name
    S127-31
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    endogenous retrovirus group K member 31
  • Synonyms gene name
    • endogenous retrovirus group K, member 31
  • GenBank acession
  • Locus
  • Discovery year
    2015-05-14
  • Pubmed identfication
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin kappa variable 3-31 (pseudogene)
  • Synonyms gene name
    • immunoglobulin kappa variable 3-31
    • immunoglobulin kappa variable 3-31 pseudogene
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-18
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin kappa locus at 2p11.2
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin lambda variable 3-31 (pseudogene)
  • Synonyms gene name
    • immunoglobulin lambda variable 3-31
    • immunoglobulin lambda variable 3-31 pseudogene
  • GenBank acession
  • Locus
  • Discovery year
    2000-05-08
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin lambda locus at 22q11.2
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    RNA, variant U1 small nuclear 31
  • Locus
  • Discovery year
    2019-07-25
  • Classification
    • Variant U1 small nuclear RNAs
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin kappa variable 3D-31 (pseudogene)
  • Synonyms gene name
    • immunoglobulin kappa variable 3D-31
    • immunoglobulin kappa variable 3D-31 pseudogene
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-18
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin kappa locus at 2p11.2
  • VEGA ID
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    olfactory receptor family 1 subfamily D member 5
  • Synonyms gene name
    • olfactory receptor, family 1, subfamily D, member 5
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1998-09-14
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Olfactory receptors, family 1
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    RNA, U6 small nuclear 31, pseudogene
  • Synonyms gene
  • Synonyms gene name
    • RNA, U6 small nuclear 31
  • Locus
  • Discovery year
    2008-06-10
  • Entrez gene record
  • RefSeq identity
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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