Mouse Anti-Rat Pannexin 2 Monoclonal IgG1, Clone S121A-31
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Catalog numberSMC-420D-A488
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PricePlease ask
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Size100 µg
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CloneS121A-31
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ImmunogenFusion protein amino acids 11-664 (all but first 10 amino acids, encompassing extracellular and cytoplasmic domains) of rat Pannexin-2
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Antibody s full descriptionMouse Anti-Rat Pannexin 2 Monoclonal IgG1 Antibody, Clone: S121A-31: ATTO 488
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Antibody s categoryMonoclonal Antibodies
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Antibody s other namePannexin 2 Antibody, PANX2 Antibody, PX2 Antibody, hPANX2 Antibody, MGC119432 Antibody
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Raised inMouse
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Antibody s targetPannexin 2
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Primary research fieldsNeuroscience, Cell Structure, Membrane Markers, Pumps/Transporters, Neurotransmitter Transporters, Cell Signaling
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF
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Antibody s reactivityHuman, Rat
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Antibody s dilutionsWB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~70kDa. Does not cross-react with Pannexin-1 or Pannexin-3.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-420 was sufficient for detection of Pannexin-2 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellCell Membrane , Cell Junction , Gap Junction
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextGap junctions are formed by a hexameric group of proteins called connexions for the transport of low molecular weight proteins from cell to cell. Connexins, which are present in all metazoan organisms, serve diverse functions ranging from control of cell growth and differentiation to electric conduction in excitable tissues. Several mammalian cells with malignant phenotypes exhibit decreased connexin expression and gap junction communication. The pannexin gene family encodes a second class of putative gap junction proteins. Pannexins are highly conserved in invertebrates and mammals, indicating the importance of their gap junctional coupling function. Mammalian Pannexin-1 and Pannexin-3 are closely related, while Pannexin-2 is more distantly related. Pannexin-2 is a transmembrane protein expressed in the central nervous system that is unable to assemble in homomeric channels but forms heteromeric channels with Pannexin-1.
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Bibliography1. Bruzzone R., et al. (2003) Proc. Natl. Acad. Sci. USA 100: 13644-13649. 2. Bao L., et al. (2004) FEBS Lett. 572: 65-68. 3. Baranova A., et al. (2004) Genomics 83: 706-716. 4. Vogt A., et al. (2005) Brain Res. Mol. Brain Res. 141: 113-120. 5. Bruzzone R., et al. (2005) J. Neurochem. 92: 1033-1043. 6. Panchin Y.V. (2005) J. Exp. Biol. 208 (Pt. 8): 1415-1419. 7. Söhl G., et al. (2005) Nature reviews. Neuroscience 6: 191-200. 8. Ray A., et al. (2005) Eur. J. Neurosci. 21: 3277-3290. 9. Barbe MT., Monyer H. and Bruzzone R. (2006) Physiology 21: 103-114.
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Released date5-Feb-2013
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_955441.2
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Gene number362979
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Protein numberP60571
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-Pannexin-2 Monoclonal Antibody, Clone S121A-31 (SMC-420). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Pannexin-2 Monoclonal Antibody (SMC-420) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasm, Nucleus. Magnification: 60X. Western Blot analysis of Rat Brain Membrane showing detection of ~70 kDa Pannexin 2 protein using Mouse Anti-Pannexin 2 Monoclonal Antibody, Clone S121A-31 (SMC-420). Lane 1: Molecular Weight (MW) Ladder. Lane 2: Rat Brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Pannexin 2 Monoclonal Antibody (SMC-420) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~70 kDa. Mouse Anti-Pannexin-2 Antibody [S121A-31] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human SK-N-BE Cells (Human Neuroblastoma cells) (SMC-420) Mouse Anti-Pannexin 2 Antibody [S121A-31] used in Western Blot (WB) on Rat Brain Membrane (SMC-420)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Rattus norvegicus
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Gene target
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Gene symbolSNORD114-31, SNORD115-31, ERVK-31, IGKV3-31, IGLV3-31, RNVU1-31, IGKV3D-31, IL31, OR1D5, RNU6-31P
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Short nameMouse Anti- Pannexin 2 Monoclonal IgG1, Clone S121A-31
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelATTO 488
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SpeciesRat, Mouses, Rats
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Alternative nameMouse Antibody toRat Pannexin 2 monoclonal IgG1, clonality S121A-31
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Alternative techniquemurine, antibodies
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Clone nameS121A-31
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Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 114-31
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Synonyms
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Locus
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Discovery year2006-09-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-31
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameendogenous retrovirus group K member 31
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Synonyms gene name
- endogenous retrovirus group K, member 31
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GenBank acession
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Locus
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Discovery year2015-05-14
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 3-31 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 3-31
- immunoglobulin kappa variable 3-31 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable 3-31 (pseudogene)
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Synonyms gene name
- immunoglobulin lambda variable 3-31
- immunoglobulin lambda variable 3-31 pseudogene
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA, variant U1 small nuclear 31
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Locus
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Discovery year2019-07-25
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Classification
- Variant U1 small nuclear RNAs
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 3D-31 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 3D-31
- immunoglobulin kappa variable 3D-31 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameinterleukin 31
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-11-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Interleukins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameolfactory receptor family 1 subfamily D member 5
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Synonyms gene name
- olfactory receptor, family 1, subfamily D, member 5
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-09-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Olfactory receptors, family 1
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 31, pseudogene
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Synonyms gene
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Synonyms gene name
- RNA, U6 small nuclear 31
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Locus
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Discovery year2008-06-10
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Entrez gene record
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RefSeq identity
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
