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Clone
S75-3
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Immunogen
Fusion protein amino acids 824-1203 (cytoplasmic C-terminus) of rat mGluR5b
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Antibody s full description
Mouse Anti-Rat mGluR5 Monoclonal IgG2A Antibody, Clone: S75-3: HRP
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
mGluR Antibody, metabotropic glutamate receptor 5 Antibody, metabotropic glutamate receptor 1 Antibody, mGlu5 Antibody, mGlu1 Antibody, mGluR5 Antibody, mGluR1 Antibody, GPRC1E Antibody, GRM5 Antibody, Metabotropic glutamate receptor 5 variant F Antibody, Metabotropic glutamate receptor 5 variant G Antibody, Metabotropic glutamate receptor 5 variant H Antibody, Glutamate receptor metabotropic 5 Antibody
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Raised in
Mouse
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Antibody s target
mGluR5
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Primary research fields
Neuroscience, Neurotransmitter Receptors, Glutamate Receptors, Metabotropic Glutamate Receptors (mGluR), Cell Signaling, Cancer
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Brandname
None
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Antibodies applications
WB, IHC, ICC/IF, IP
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s dilutions
WB (1:1000), IHC (1:1000), ICC/IF (1:1000); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~130kDa (both mGluR1 and mGluR5).
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
1 µg/ml of SMC-419 was sufficient for detection of mGluR1/5 glutamate receptor in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cell
Cell Membrane
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
The AMPA receptor is a non-NMDA-type ionotropic transmembrane receptor for glutamate that mediates fast synaptic transmission in the CNS. AMPARs are composed of four types of subunits, designated as GluR1, GluR2, GluR3 and GluR4, which combine to form tetramers (1, 2).
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Bibliography
1. Mayer M.L. (2005) Current Opinion in Neurobio. 15(3): 282-288. 2. Keinane K., et al. (1990) Science. 249: 556-560.
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Released date
13-Mar-2013
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
NP_058708.1
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Gene number
24418
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Protein number
P31424
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Antibody s datasheet
Contact our support service
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-mGluR1/5 glutamate receptor Monoclonal Antibody, Clone S75-3 (SMC-419). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-mGluR1/5 glutamate receptor Monoclonal Antibody (SMC-419) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasm, Nucleus. Magnification: 60X. Western Blot analysis of Rat Brain Membrane showing detection of ~130 kDa mGluR5 protein using Mouse Anti-mGluR5 Monoclonal Antibody, Clone S75-33 (SMC-419). Lane 1: Molecular Weight (MW) Ladder. Lane 2: Rat Brain Membrane. Load: 10 µg. Block: 5% milk. Primary Antibody: Mouse Anti-mGluR5 Monoclonal Antibody (SMC-419) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~130 kDa. Mouse Anti-mGluR1/5 glutamate receptor Antibody [S75-3] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human SK-N-BE Cells (Human Neuroblastoma cells) (SMC-419) Mouse Anti-mGluR1/5 Antibody [S75-33] used in Western Blot (WB) on Rat Brain Membrane (SMC-419)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Rattus norvegicus
