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Clone
Ly1C6
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Immunogen
Rat liver lysosomal membrane preparations
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Antibody s full description
Mouse Anti-Rat LAMP1 Monoclonal IgG1 Antibody, Clone: Ly1C6: Unconjugated
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
CD107 Antibody, CD107a Antibody, LAMPA Antibody, LGP120 Antibody, IgpA Antibody, Lysosome-associated membrane glycoprotein 1 Antibody, CD107 antigen-like family member A Antibody, 120 kDa lysosomal membrane glycoprotein Antibody, Lamp-1 Antibody
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Raised in
Mouse
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Antibody s target
LAMP1
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Primary research fields
Cell Signaling, Organelle Markers, Trafficking, Chaperones, Neuroscience
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Brandname
None
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Antibodies applications
WB, ICC/IF, IP
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Antibody s reactivity
Human, Mouse, Rat, Hamster
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Antibody s dilutions
WB (1:1000), ICC/IF (1:1000); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~120kDa.
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
1 µg/ml was sufficient for detection of LAMP1 in rat liver miscrosome by ECL immunoblot analysis.
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Antibody in cell
Cell Membrane , Endosome , Lysosome , Endosome membrane , Lysosome membrane
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37% sequence homology (2). They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane (3). Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxyl¬terminal tyrosine ba based motif (YXXI) (1). If there is a disturbance in this spacing, lysosome localization of LAMP1 is abolished and the mutant protein then cycles between the membrane and the endosome (3). This stimulation has also been shown to have an associated release of histamine, leukotriene C (4) and prostaglandin D (2), which shows that LAMP-1 and LAMP-2 are activation markers for normal mast cells (1). They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation (4).
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Bibliography
1. Grutzkau, A. et al (2004) Cytometry A. 61(10): 62-68. 2. Furuta, K. et al. (1999) EMBO J. 17(5):1304-14. 3. Rohrer, J. et al. (1996) J Cell Biol. 132(4): 565-76. 4. Kannan, K., et al. (1996) Cell Immunol. 171: 10-19. 5. Lewis, V., et al. (1985) J. Cell Biol. 100: 1839-1847. 6. Jones, K.A., et al. (2004) Exp Cell Res 295(2): 512-524.
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Released date
1-Nov-2007
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PubMed number
24907298, 24989000
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Tested applications
Immunocytochemistry/Immunofluorescence, Immunocytochemistry/Immunofluorescence
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Tested reactivity
Human, Mouse
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NCBI number
NP_036989.1
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Gene number
25328
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Protein number
P14562
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Antibody s datasheet
Contact our support service
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-LAMP1 Monoclonal Antibody, Clone Ly1C6 (SMC-140). Tissue: transfected HeLa cells. Species: Human. Primary Antibody: Mouse Anti-LAMP1 Monoclonal Antibody (SMC-140) at 1:1000. Secondary Antibody: APC Goat Anti-Mouse (red). Courtesy of: Robert H Edwards, U. of Cali, San Fran School of Medicine. Western Blot analysis of Rat liver microsome lysate showing detection of LAMP1 protein using Mouse Anti-LAMP1 Monoclonal Antibody, Clone Ly1C6 (SMC-140). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-LAMP1 Monoclonal Antibody (SMC-140) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-LAMP1 Antibody [Ly1C6] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human transfected HeLa cells (SMC-140) Mouse Anti-LAMP1 Antibody [Ly1C6] used in Western Blot (WB) on Rat liver microsome lysate (SMC-140)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Rattus norvegicus
