Mouse Anti-Rat Kv3.2 Monoclonal IgG1, Clone S410-17

  • Catalog number
    SMC-492D-ALP
  • Price
    Please ask
  • Size
    100 µg
  • Clone
    S410-17
  • Immunogen
    Fusion protein amino acids 474-613 (Cytoplasmic C-terminus) of rat Kv3.2a
  • Antibody s full description
    Mouse Anti-Rat Kv3.2 Monoclonal IgG1 Antibody, Clone: S410-17: Alkaline Phosphatase
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    Potassium voltage-gated channel subfamily C member 2 Antibody, Kcnc2 Antibody, KSHIIIA Antibody
  • Raised in
    Mouse
  • Antibody s target
    Kv3.2
  • Primary research fields
    Neuroscience, Ion channels, Potassium Channels, Voltage-Gated Potassium Channels
  • Brandname
    None
  • Antibodies applications
    WB, IHC, ICC/IF
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s dilutions
    WB (1:1000); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH7.4, 50% glycerol, 0.1% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~80-100kDa. Varies due to post-translational modifications.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    A 1:100 dilution of SMC-492 was sufficient for detection of Kv3.2 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cell Membrane
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    Kv3.2, a member of the KCNC2 gene family, belongs to a group of potassium channels that contribute to the maintenance of cell volume, membrane potential, neuronal excitability and the secretion of transmitters, salt and hormones. Kv3.2 are predominantly expressed in neurons that fire at high frequencies, and in the fast spiking GABAergic interneurons of the neocortex, hippocampus, and caudate nucleus (1-3). They specifically functioning as a delayed rectifier activated by large membrane depolarizations (2, 4).
  • Bibliography
    1. Kolodin Y.O. (2008) more 2. Rudy B., McBain C.J. (2001) Trends Neurosci. 24(9): 517-526. 3. Gutman G.A., et al. (2005) Pharmacol Rev. 57(4): 472-508. 4. Rudy B, et al. (1999) Ann. NY Acad. Sci. 868: 304-343.
  • Released date
    10-Mar-2015
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    NP_631962.1
  • Gene number
    246153
  • Protein number
    P22462
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Kv3.2 Monoclonal Antibody, Clone S410-17 (SMC-492). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Kv3.2 Monoclonal Antibody (SMC-492) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Kv3.2 Antibody (D) Composite. Western Blot analysis of Mouse, Rat Brain showing detection of ~80-100 kDa Kv3.2 protein using Mouse Anti-Kv3.2 Monoclonal Antibody, Clone S410-17 (SMC-492). Lane 1: MW Ladder. Lane 2: Mouse Brain. Lane 3: Rat Brain Membrane. Lane 4: Rat Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Kv3.2 Monoclonal Antibody (SMC-492) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~80-100 kDa. Other Band(s): ~30 kDa for Rat Brain . Mouse Anti-Kv3.2 Antibody [S410-17] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-492) Mouse Anti-Kv3.2 Antibody [S410-17] used in Western Blot (WB) on Mouse, Rat Brain (SMC-492)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus, Rattus norvegicus
  • Gene target
    Kv3   Monoclonal   IgG1   S410-17  
  • Gene symbol
    PIRC95, PIRC91, PIRC90, PIRC93, PIRC92, SNORD115-17, IGHV3OR16-17, SNORD116-17
  • Short name
    Mouse Anti- Kv3 2 Monoclonal IgG1, Clone S410-17
  • Technique
    anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
  • Host
    mouse
  • Isotype
    IgG1, IgG1
  • Label
    Alkaline Phosphatase
  • Species
    Rat, Mouses, Rats
  • Alternative name
    Mouse Antibody toRat Kv3.2 monoclonal IgG1, clonality S410-17
  • Alternative technique
    murine, antibodies
  • Clone name
    S410-17
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 95
  • Locus
    17
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 91
  • Locus
    17
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 90
  • Locus
    17
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 93
  • Locus
    17
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 92
  • Locus
    17
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin heavy variable 3/OR16-17 (non-functional)
  • Synonyms
  • Locus
  • Discovery year
    2020-06-26
  • Entrez gene record
  • Classification
    • Immunoglobulin heavy (IGH) orphons
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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