Mouse Anti-Rat Kv3.2 Monoclonal IgG1, Clone S410-17
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Catalog numberSMC-492D-ALP
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PricePlease ask
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Size100 µg
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CloneS410-17
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ImmunogenFusion protein amino acids 474-613 (Cytoplasmic C-terminus) of rat Kv3.2a
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Antibody s full descriptionMouse Anti-Rat Kv3.2 Monoclonal IgG1 Antibody, Clone: S410-17: Alkaline Phosphatase
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Antibody s categoryMonoclonal Antibodies
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Antibody s other namePotassium voltage-gated channel subfamily C member 2 Antibody, Kcnc2 Antibody, KSHIIIA Antibody
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Raised inMouse
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Antibody s targetKv3.2
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Primary research fieldsNeuroscience, Ion channels, Potassium Channels, Voltage-Gated Potassium Channels
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.1% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~80-100kDa. Varies due to post-translational modifications.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysisA 1:100 dilution of SMC-492 was sufficient for detection of Kv3.2 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellCell Membrane
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextKv3.2, a member of the KCNC2 gene family, belongs to a group of potassium channels that contribute to the maintenance of cell volume, membrane potential, neuronal excitability and the secretion of transmitters, salt and hormones. Kv3.2 are predominantly expressed in neurons that fire at high frequencies, and in the fast spiking GABAergic interneurons of the neocortex, hippocampus, and caudate nucleus (1-3). They specifically functioning as a delayed rectifier activated by large membrane depolarizations (2, 4).
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Bibliography1. Kolodin Y.O. (2008) more 2. Rudy B., McBain C.J. (2001) Trends Neurosci. 24(9): 517-526. 3. Gutman G.A., et al. (2005) Pharmacol Rev. 57(4): 472-508. 4. Rudy B, et al. (1999) Ann. NY Acad. Sci. 868: 304-343.
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Released date10-Mar-2015
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_631962.1
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Gene number246153
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Protein numberP22462
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-Kv3.2 Monoclonal Antibody, Clone S410-17 (SMC-492). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Kv3.2 Monoclonal Antibody (SMC-492) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Kv3.2 Antibody (D) Composite. Western Blot analysis of Mouse, Rat Brain showing detection of ~80-100 kDa Kv3.2 protein using Mouse Anti-Kv3.2 Monoclonal Antibody, Clone S410-17 (SMC-492). Lane 1: MW Ladder. Lane 2: Mouse Brain. Lane 3: Rat Brain Membrane. Lane 4: Rat Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Kv3.2 Monoclonal Antibody (SMC-492) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~80-100 kDa. Other Band(s): ~30 kDa for Rat Brain . Mouse Anti-Kv3.2 Antibody [S410-17] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-492) Mouse Anti-Kv3.2 Antibody [S410-17] used in Western Blot (WB) on Mouse, Rat Brain (SMC-492)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Rattus norvegicus
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Gene target
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Gene symbolPIRC95, PIRC91, PIRC90, PIRC93, PIRC92, SNORD115-17, IGHV3OR16-17, SNORD116-17
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Short nameMouse Anti- Kv3 2 Monoclonal IgG1, Clone S410-17
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelAlkaline Phosphatase
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SpeciesRat, Mouses, Rats
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Alternative nameMouse Antibody toRat Kv3.2 monoclonal IgG1, clonality S410-17
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Alternative techniquemurine, antibodies
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Clone nameS410-17
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Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 95
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 91
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 90
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 93
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 92
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-17
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3/OR16-17 (non-functional)
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Synonyms
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Locus
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Discovery year2020-06-26
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Entrez gene record
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Classification
- Immunoglobulin heavy (IGH) orphons
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 116-17
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data