Mouse Anti-Rat KCC2 Monoclonal IgG2a, Clone S1-12

  • Catalog number
    SMC-392D-A594
  • Price
    Please ask
  • Size
    100 µg
  • Clone
    S1-12
  • Immunogen
    Fusion protein amino acids 932-1043 corresponding to rat KCC2
  • Antibody s full description
    Mouse Anti-Rat KCC2 Monoclonal IgG2a Antibody, Clone: S1-12: ATTO 594
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    Potassium Chloride Cotransporter Antibody, Potassium chloride transporter 5 Antibody, SLC12A5 Antibody, hKCC2 Antibody, S12A5 Antibody, Solute carrier family 12 member 5 Antibody, Electroneutral potassium-chloride cotransporter 2 Antibody, Furosemide-sensitive K-Cl cotransporter Antibody, K-Cl cotransporter 2 Antibody, rKCC2 Antibody, Neuronal K-Cl cotransporter Antibody, KIAA1176 Antibody, Electroneutral potassium chloride cotransporter 2 Antibody, Erythroid K Cl cotransporter 2 Antibody, Furosemide sensitive K Cl cotransporter Antibody, K-Cl cotransporter 2 Antibody, KCC 2 Antibody, Neuronal K Cl cotransporter Antibody, Solute carrier family 12 (potassium chloride transporter) member 5 Antibody, Solute carrier family 12 member 5 Antibody
  • Raised in
    Mouse
  • Antibody s target
    KCC2
  • Primary research fields
    Neuroscience, Pumps/Transporters, Ion Pumps/Transporters
  • Brandname
    None
  • Antibodies applications
    WB, IHC, ICC/IF, IP
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s dilutions
    WB (1:1000), IHC (1:300), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH7.4, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~140kDa.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    1 µg/ml of SMC-392 was sufficient for detection of KCC2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    Membrane
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    KCC2 is a member of the cation-chloride cotransporter gene family (1). It acts as a K-Cl cotransporter. KCCs normally lower intracellular chloride concentrations below the electrochemical equilibrium potential and depending on the chemical concentration gradients of potassium and chloride, KCC2 can operate as a net efflux or influx pathway. It is proposed to act as the main chloride extruder to promote fast hyperpolarizing postsynaptic inhibition in the brain (2, 3). KCC2 is expressed at high levels in neurons throughout the nervous system and immunofluorescence shows that the protein is localized at inhibitory synapses of the spinal cord (4). Studies in mice have shown that KCC2 reduces GABA's inhibitory signaling, resulting in motor defects, epilepsy, and anxiety-like behavior.
  • Bibliography
    1. Lee L.H., Walker J.A., Williams J.R., Goodier R.J., Payne J.A., Moss S.J. (2007) J Biol Chem. 282(41): 29777-29784. 2. Watanabe M., Wake H., Moorhouse A.J., Nabekura J. (2009) J Biol Chem. 284(41): 27980-27988. 3. Gulyas A.I., Sik A., Payne J.A., Kaila K., Freund T.F. (2001) Eur J Neurosci. 13(12): 2205-2217. 4. Vinay L., Jean-Xavier C. (2008) Brain Res Rev. 57(1): 103-110.
  • Released date
    16-May-2012
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    NP_599190
  • Gene number
    171373
  • Protein number
    Q63633
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-KCC2 Monoclonal Antibody, Clone S1-12 (SMC-392). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-KCC2 Monoclonal Antibody (SMC-392) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) KCC2 Antibody (D) Composite. Western Blot analysis of Rat brain membrane lysate showing detection of KCC2 protein using Mouse Anti-KCC2 Monoclonal Antibody, Clone S1-12 (SMC-392). Primary Antibody: Mouse Anti-KCC2 Monoclonal Antibody (SMC-392) at 1:1000. Mouse Anti-KCC2 Antibody [S1-12] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human SK-N-BE Cells (Human Neuroblastoma cells) (SMC-392) Mouse Anti-KCC2 Antibody [S1-12] used in Western Blot (WB) on Rat brain membrane lysate (SMC-392)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus, Rattus norvegicus
  • Gene target
    KCC2   Monoclonal   IgG2a   S1-12  
  • Gene symbol
    SLC12A5, PSMD1, PIRC55, PIRC54, PIRC53, PIRC57, PIRC56, SNORD116-12, IGHV3OR16-12
  • Short name
    Mouse Anti- KCC2 Monoclonal IgG2a, Clone S1-12
  • Technique
    anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
  • Host
    mouse
  • Isotype
    IgG2a, IgG2a
  • Label
    ATTO 594
  • Species
    Rat, Mouses, Rats
  • Alternative name
    Mouse Antibody toRat KCC2 monoclonal IgG2a, clonality S1-12
  • Alternative technique
    murine, antibodies
  • Clone name
    S1-12
Gene info
  • Identity
  • Gene
  • Long gene name
    solute carrier family 12 member 5
  • Synonyms gene name
    • solute carrier family 12 (potassium/chloride transporter), member 5
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2000-11-09
  • Entrez gene record
  • Classification
    • Solute carriers
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    proteasome 26S subunit, non-ATPase 1
  • Synonyms gene name
    • proteasome (prosome, macropain) 26S subunit, non-ATPase, 1
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1995-11-28
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Proteasome
    • Armadillo like helical domain containing
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 55
  • Locus
    12
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 54
  • Locus
    12
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 53
  • Locus
    12
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 57
  • Locus
    12
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 56
  • Locus
    12
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin heavy variable 3/OR16-12 (non-functional)
  • Synonyms gene name
    • immunoglobulin heavy variable 3/OR16-12
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-17
  • Entrez gene record
  • Classification
    • Immunoglobulin heavy (IGH) orphons
  • VEGA ID
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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