Mouse Anti-Rat Chapsyn-110 Monoclonal IgG1, Clone S18-30

• Catalog number
  SMC-325D-PCP
• Price
  Please ask
• Size
  100 µg

• Clone
  S18-30
• Immunogen
  Fusion protein amino acids 1-852 of rat Chapsyn-110
• Antibody s full description
  Mouse Anti-Rat Chapsyn-110 Monoclonal IgG1 Antibody, Clone: S18-30: PerCP
• Antibody s category
  Monoclonal Antibodies
• Antibody s other name
  PSD93 Antibody, Chapsyn-110 Antibody, MGC131811 Antibody, PSD-93 Antibody, Discs Large Homolog 2 Antibody, DLG2 Antibody, Postsynaptic density protein PSD-93 Antibody, DLGH2 Antibody, Channel-associated protein of synapse-110 Antibody
• Raised in
  Mouse
• Antibody s target
  Chapsyn-110
• Primary research fields
  Neuroscience, Cell Structure, Post-Synaptic Markers, Cell Signaling, Scaffolds
• Brandname
  None
• Antibodies applications
  WB, IHC, IP, ICC/IF, AM
• Antibody s reactivity
  Human, Mouse, Rat
• Antibody s dilutions
  WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Purity
  Protein G Purified
• Antibody buffer for storage
  PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Antibody s concentration
  1 mg/ml
• Antibody s specificity
  Detects ~110kDa. No cross-reactivity against other MAGUK family members, PSD95, SAP97, SAP102, expressed in transfected cells. Weak human detection.
• Storage recommendations
  -20ºC
• Shipping recommendations
  Blue Ice or 4ºC
• Antibody certificate of analysis
  1 µg/ml of SMC-325 was sufficient for detection of Chapsyn -110 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Antibody in cell
  Membrane , Cell Junction , Synapse , Postsynaptic Cell Membrane , Cell Projection , Axon
• Tissue specificity
  See included datasheet or contact our support service
• Scientific context
  Chapsyn-110, is a part of the membrane-associated putative guanylate kinase (MAGUK) family. It binds directly to the NMDA receptor, and Shaker K+ channel subunits, and is 70-80% identical to PSD-95/SAP90 and SAP97 (1). It associated tightly with the postsynaptic density I brain, and mediates the clustering of both NMDA recpetors and K+ channels in heterologous cells. The encoded protein forms a heterodimer with PSD-95 that may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins (1, 2).
• Bibliography
  1. Kim E., et al. (1996) Neuron. 17:103-113. 2. Godreau D., Neyroud N., Vranckx R. and Hatem S. (2004) Med Sci (Paris). 20(1): 84-88.
• Released date
  13-Apr-2010
• Tested applications
  To be tested
• Tested reactivity
  To be tested
• NCBI number
  NP_071618.1
• Gene number
  64053
• Protein number
  Q63622
• Antibody s datasheet
  Contact our support service
• Representative figure link
  more | details
• Representative figure legend
  Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Chapsyn-110 Monoclonal Antibody, Clone S18-30 (SMC-325). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Chapsyn-110 Monoclonal Antibody (SMC-325) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Membrane, Cell Junction, Synapse, Postsynaptic Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Chapsyn-110 Antibody (D) Composite. Western Blot analysis of Rat brain membrane lysate showing detection of Chapsyn-110 protein using Mouse Anti-Chapsyn-110 Monoclonal Antibody, Clone S18-30 (SMC-325). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Chapsyn-110 Monoclonal Antibody (SMC-325) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-Chapsyn-110 Antibody [S18-30] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human SK-N-BE Cells (Human Neuroblastoma cells) (SMC-325) Mouse Anti-Chapsyn-110 Antibody [S18-30] used in Western Blot (WB) on Rat brain membrane lysate (SMC-325)
• Warning information
  Non-hazardous
• Country of production
  Canada
• Total weight kg
  1.4
• Net weight g
  0.1
• Stock availability
  In Stock
• Other related products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus, Rattus norvegicus

• Gene target
  Chapsyn-110   Monoclonal   IgG1   S18-30  
• Gene symbol
  SNORD114-30, SNORD116-30, SNORD115-30, IGKV2-30, RNU6-30P, IGHV3-30, IGLV3-30, IGHVII-30-21, IGHVII-30-1, IGKV2D-30
• Short name
  Mouse Anti- Chapsyn-110 Monoclonal IgG1, Clone S18-30
• Technique
  anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
• Host
  mouse
• Isotype
  IgG1, IgG1
• Label
  PerCP
• Species
  Rat, Mouses, Rats
• Alternative name
  Mouse Antibody toRat Chapsyn-110 monoclonal IgG1, clonality S18-30
• Alternative technique
  murine, antibodies
• Clone name
  S18-30

Gene info

• Identity
  HGNC:33018
• Gene
  SNORD114-30
• Long gene name
  small nucleolar RNA, C/D box 114-30
• Synonyms
  • 14q(II-30)
• Locus
  14q32.31
• Discovery year
  2006-09-26
• Entrez gene record
  767611
• Pubmed identfication
  • 12045206
• RefSeq identity
  • NR_003223.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:42797
• Gene
  SNORD116-30
• Long gene name
  small nucleolar RNA, C/D box 116-30
• Synonyms
  • HBII-85-30
• Locus
  15q11.2
• Discovery year
  2011-08-22
• Entrez gene record
  100873856
• RefSeq identity
  • NR_047032
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:33049
• Gene
  SNORD115-30
• Long gene name
  small nucleolar RNA, C/D box 115-30
• Synonyms
  • HBII-52-30
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-15
• Entrez gene record
  100033804
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003345.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:5785
• Gene
  IGKV2-30
• Long gene name
  immunoglobulin kappa variable 2-30
• GenBank acession
  • X63403
• Locus
  2p11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28919
• RefSeq identity
  • NG_000834
• Classification
  • Immunoglobulin kappa locus at 2p11.2
• VEGA ID
  OTTHUMG00000151692

Gene info

• Identity
  HGNC:34274
• Gene
  RNU6-30P
• Long gene name
  RNA, U6 small nuclear 30, pseudogene
• Synonyms gene
  • RNU6-30
• Synonyms gene name
  • RNA, U6 small nuclear 30
• Locus
  Xq22.1
• Discovery year
  2008-06-10
• Entrez gene record
  100873747
• RefSeq identity
  • NR_046490

Gene info

• Identity
  HGNC:5591
• Gene
  IGHV3-30
• Long gene name
  immunoglobulin heavy variable 3-30
• GenBank acession
  • M83134
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  28439
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000188332

Gene info

• Identity
  HGNC:5912
• Gene
  IGLV3-30
• Long gene name
  immunoglobulin lambda variable 3-30 (pseudogene)
• Synonyms gene name
  • immunoglobulin lambda variable 3-30
  • immunoglobulin lambda variable 3-30 pseudogene
• GenBank acession
  • Z73646
• Locus
  22q11.22
• Discovery year
  2000-05-08
• Entrez gene record
  28789
• RefSeq identity
  • NG_000002
• Classification
  • Immunoglobulin lambda locus at 22q11.2
• VEGA ID
  OTTHUMG00000151182

Gene info

• Identity
  HGNC:52400
• Gene
  IGHVII-30-21
• Long gene name
  immunoglobulin heavy variable (II)-30-21 (pseudogene)
• Synonyms
  • IGHV(II)-30-21
• Locus
  14q32.33
• Discovery year
  2016-11-10
• Entrez gene record
  107548099
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33

Gene info

• Identity
  HGNC:5676
• Gene
  IGHVII-30-1
• Long gene name
  immunoglobulin heavy variable (II)-30-1 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (II)-30-1
  • immunoglobulin heavy variable (II)-30-1 pseudogene
• Synonyms
  • IGHV(II)-30-1
• GenBank acession
  • AB019439
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28371
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152099

Gene info

• Identity
  HGNC:5801
• Gene
  IGKV2D-30
• Long gene name
  immunoglobulin kappa variable 2D-30
• GenBank acession
  • X63402
• Locus
  2p11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28881
• RefSeq identity
  • NG_000833
• Classification
  • Immunoglobulin kappa locus at 2p11.2
• VEGA ID
  OTTHUMG00000151613

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products