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Clone
S57-46
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Immunogen
Fusion protein amino acids 1507-1733 (intracellular carboxyl terminus) of rabbit Cav1.2
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Antibody s full description
Mouse Anti-Rabbit Cav1.2 Monoclonal IgG1 Antibody, Clone: S57-46: ATTO 633
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
CACH3 Antibody, CACN4 Antibody, CACNA 1D Antibody, CACNL1A2 Antibody, voltage dependent L type calcium channel subunit alpha 1D Antibody, alpha-1 subunit voltage-dependent calcium channel Antibody, calcium channel voltage-dependent L type alpha 1C subunit1 Antibody, voltage-gated calcium channel alpha subunit Cav1.2 Antibody, calcium channel L type Antibody, alpha 1 polypeptide isoform 1 cardic muscle Antibody, calcium channel cardic dihydropyridine-sensitive alpha-1 subunit Antibody, voltage-gated L-type calcium channel Cav1.2 alpha 1 subunit splice variant 10 Antibody, DHPR alpha-1 subunit Antibody, Voltage-gated calcium channel subunit alpha Cav1.2 Antibody, Calcium channel L type alpha-1 polypeptide isoform 1 cardiac muscle Antibody
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Raised in
Mouse
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Antibody s target
Cav1.2
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Primary research fields
Neuroscience, Ion Channels, Calcium Channels, Voltage-Gated Calcium Channels, Cancer, Cell Signaling, cardiovascular System, Heart
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Brandname
None
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Antibodies applications
WB, IHC, ICC/IF, IP, AM
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Antibody s reactivity
Human, Mouse, Rat, Hamster
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Antibody s dilutions
WB (1:1000), IHC (1:1000), ICC/IF (1:100), IP (1:200); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
PBS pH7.4, 50% glycerol, 0.1% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~240kDa (varies with cell background due to glycosylation).
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
1 µg/ml of SMC-300 was sufficient for detection of Cav1.2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cell
Membrane , Cell Membrane
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
Cav1.2 is a cardiac L-type calcium channel, and is important for excitation and contraction of the heart (1). It may be associated with a variant of Long QT syndrome called Timothy's syndrome (2, 3) and also with Brugada syndrome. Some references also suggest it is related to bipolar disease as well (3).
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Bibliography
1. Splawski I., et al. (2004) Cell. 119 (1): 19-31. 2. Krey J.F., and Dolmetsch R. (2009) Biophysical. 96 (3): 221a-222a. 3. Crotti L., Celano G., Dagradi F. and Schwartz P.J. (2008) Orphanet J Rare Disease 3:18.
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Released date
6-Jul-2009
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
NP_001129994.1
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Gene number
100144322
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Protein number
P15381
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Antibody s datasheet
Contact our support service
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav1.2 Monoclonal Antibody, Clone S57-46 (SMC-300). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Cav1.2 Monoclonal Antibody (SMC-300) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Membrane, Cytoplasm, Nucleoplasm. Magnification: 60X. (A) Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain. (B) Anti-Cav1.2 Antibody. (C) Composite. Immunohistochemistry analysis using Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody, Clone S57-46 (SMC-300). Tissue: hippocampus. Species: Human. Fixation: 10% formalin. Primary Antibody: Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody (SMC-300) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Immunoprecipitation analysis using Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody, Clone S57-46 (SMC-300). Tissue: INS-1E cells. Species: Rat. Primary Antibody: Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody (SMC-300) at 1:200. Courtesy of: Merrie Mosedale. Western Blot analysis of Hamster T-CHO cell lysate showing detection of CaV1.2 Calcium Channel protein using Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody, Clone S57-46 (SMC-300). Primary Antibody: Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody (SMC-300) at 1:1000. Immunohistochemistry analysis using Mouse Anti-CaV1.2 Calcium channel Monoclonal Antibody, Clone S57-47 (SMC-300). Tissue: Brain Tissue. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-CaV1.2 Calcium channel Monoclonal Antibody (SMC-300) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Magnification: 40x. Mouse Anti-Cav1.2 Antibody [S57-46] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human SK-N-BE Cells (Human Neuroblastoma cells) (SMC-300) Mouse Anti-CaV1.2 Calcium Channel Antibody [S57-46] used in Immunohistochemistry (IHC) on Human hippocampus (SMC-300) Mouse Anti-CaV1.2 Calcium Channel Antibody [S57-46] used in Immunoprecipitation (IP) on Rat INS-1E cells (SMC-300) Mouse Anti-CaV1.2 Calcium Channel Antibody [S57-46] used in Western Blot (WB) on Hamster T-CHO cell lysate (SMC-300) Mouse Anti-CaV1.2 Calcium channel antibody [S57-47] used in Immunohistochemistry (IHC) on Mouse Brain Tissue (SMC-300)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Oryctolagus cuniculus
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Gene target
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Gene symbol
IGLV7-46, IGHV1-46, IGHVII-46-1, SNORD115-46, RNU6-46P, LAMP5, OR7E110P
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Short name
Mouse Anti-Rabbit Cav1 2 Monoclonal IgG1, Clone S57-46
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Technique
Rabbit, anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Host
mouse, Rabbits
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Isotype
IgG1, IgG1
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Label
ATTO 633
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Species
Mouse, Mouses
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Alternative name
Mouse Antibody tohost: rabbit caveolin 1, caveolae protein, 22kDa.2 monoclonal IgG1, clonality S57-46
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Alternative technique
rabbit-anti, murine, antibodies
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Alternative to gene target
caveolin 1, caveolae protein, 22kDa, BSCL3 and CGL3 and LCCNS and MSTP085 and PPH3 and VIP21, CAV1 and IDBG-37314 and ENSG00000105974 and 857, nitric-oxide synthase binding, Cell surfaces, Cav1 and IDBG-129000 and ENSMUSG00000007655 and 12389, CAV1 and IDBG-632834 and ENSBTAG00000017869 and 281040
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Clone name
S57-46
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