Mouse Anti Rabbit Antibody H

Mouse Anti Rabbit Antibody H is available 6 times from Bioss secondary antibodies labs

bs-0295M | Mouse Anti-Rabbit IgG Antibody (H+L)size: 1mg | 164.24 USD

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bs-0295M-Biotin | Mouse Anti-Rabbit IgG Antibody (H+L), Biotin Conjugatedsize: 0.2ml | 140.94 USD

  • Category
    Conjugated Secondary Antibody
  • Conjugation
    Biotin
  • Target antigen
    Rabbit IgG
  • Concentration
    1ug/ul
  • Background of the target antigen
    Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • Properties
    If you buy Antibodies supplied by Bioss Secondary Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
  • Additional conjugation
    Biotinylated
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rabbits are used for polyclonal antibody production by Bioss Secondary Antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. Bioss Secondary Antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Additional isotype
    IgG
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus,Oryctolagus cuniculus
  • French translation
    anticorps
  • Gene target
  • Short name
    Mouse Anti-Rabbit IgG Antibody (H+L) Conjugated
  • Technique
    Antibody, Rabbit, Mouse, anti, IgG
  • Isotype
    IgG
  • Label
    Biotin
  • Alternative name
    Mouse antibody to-production species: rabbit Immunoglobulin G (Antibody to) (heavy and light chain), biotinilated coupled
MeSH Data
  • Name
  • Concept
    Scope note:Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
Additional information

bs-0295M-HRP | Mouse Anti-Rabbit IgG Antibody (H+L), HRP Conjugatedsize: 0.2ml | 140.94 USD

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bs-0369M | Mouse Anti-Rabbit IgM Antibody (H+L)size: 1mg | 164.24 USD

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bs-0369M-Biotin | Mouse Anti-Rabbit IgM Antibody (H+L), Biotin Conjugatedsize: 0.2ml | 140.94 USD

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bs-0369M-HRP | Mouse Anti-Rabbit IgM Antibody (H+L), HRP Conjugatedsize: 0.2ml | 140.94 USD

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  • Host Organism
    Mouse
  • Modification
    Unmodified
  • Modification site
    None
  • Clonality
    Polyclonal antibody
  • Clone
    Polyclonal antibody
  • Tested applications
    WB, ELISA, IHC-P, IHC-F
  • Recommended dilutions
    WB(1:500-2000), ELISA(1:500-2000), IHC-P(1:500-2000), IHC-F(1:500-2000)
  • Cross reactivity
    Rabbit
  • Purification method
    This antibody has been purified via Protein A.
  • Storage conditions
    Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to one year.
  • Host
    mouse
  • Species
    Mouse
  • Alternative technique
    antibodies
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