Mouse Anti-Plant HSP70/HSC70 Monoclonal IgG1, Clone 5G1-95

• Catalog number
  SMC-120D-P594
• Price
  Please ask
• Size
  100 µg

• Clone
  5G1-95
• Immunogen
  Purified HSP70 from Phaseolus aureus (mung bean)
• Antibody s full description
  Mouse Anti-Plant HSP70/HSC70 Monoclonal IgG1 Antibody, Clone: 5G1-95: PE/ATTO 594
• Antibody s category
  Monoclonal Antibodies
• Antibody s other name
  HSC54 Antibody, HSC70 Antibody, HSC71 Antibody, HSP70 1 Antibody, HSP701/HSP70 2 Antibody, HSP70.1 Antibody, HSP71 Antibody, HSP72 Antibody, HSP73 Antibody, HSPA1 Antibody, HSPA10 Antibody, HSPA1A Antibody, HSPA1B Antibody, LAP1 Antibody, NIP71 Antibody
• Raised in
  Mouse
• Antibody s target
  HSP70/HSC70
• Primary research fields
  Cancer, Heat Shock
• Brandname
  None
• Antibodies applications
  WB, ELISA
• Antibody s reactivity
  Plant
• Antibody s dilutions
  WB (1:1000); optimal dilutions for assays should be determined by the user.
• Purity
  Protein G Purified
• Antibody buffer for storage
  PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Antibody s concentration
  1 mg/ml
• Antibody s specificity
  Detects ~70kDa. Recognizes constitutive and inducible plants HSP70 (HSC70/HSP72). Does not cross-react with Human, Rat, bacteria (DNAK) or Human Bip.
• Storage recommendations
  -20ºC
• Shipping recommendations
  Blue Ice or 4ºC
• Antibody certificate of analysis
  1 µg/ml of SMC-120 was sufficient for detection of HSP70/HSC70 in 20 µg of Phaseolus aureus (mung bean) lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Antibody in cell
  Cytoplasm
• Tissue specificity
  See included datasheet or contact our support service
• Scientific context
  HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (1). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (2). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (3). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (4). All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (5). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. For more information visit our HSP70 Scientific Resource Guide at more.
• Bibliography
  1. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J. Mol. Evol.38 (1): 1-17. 2. Rothman J. (1989) Cell 59: 591-601. 3. DeLuca-Flaherty et al. (1990), Cell 62: 875-887. 4. Bork P., Sander C. & Valencia A. (1992) Proc. Nut1 Acad. Sci. USA 89: 7290-7294. 5. Fink A.L. (1999) Physiol. Rev. 79: 425-449.
• Released date
  1-Sep-2011
• Tested applications
  To be tested
• Tested reactivity
  To be tested
• NCBI number
  Refer to NCBI
• Gene number
  Refer to GenBank
• Protein number
  Refer to Swiss-Prot
• Antibody s datasheet
  Contact our support service
• Representative figure link
• Representative figure legend
  Western Blot analysis of Duckweed (Lemma minor) Heat Shocked cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone 5G1-95 (SMC-120). Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (SMC-120) at 1:1000. Mouse Anti-Hsp70 Antibody [5G1-95] used in Western Blot (WB) on Duckweed (Lemma minor) Heat Shocked cell lysates (SMC-120)
• Warning information
  Non-hazardous
• Country of production
  Canada
• Total weight kg
  1.4
• Net weight g
  0.1
• Stock availability
  In Stock
• Other related products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Plants RNA, DNA , cDNA and plant tissues are used to study plant genes.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  HSP70/HSC70   Monoclonal   IgG1   5G1  
• Gene symbol
  HSPA1B, HSPA1A, HSPA14, HSPA1L, HSPA8
• Short name
  Mouse Anti- HSP70/HSC70 Monoclonal IgG1, Clone 5G1-95
• Technique
  anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
• Host
  mouse
• Isotype
  IgG1, IgG1
• Label
  PE/ATTO 594
• Species
  Plant, Mouses, Plants
• Alternative name
  Mouse Antibody toPlant HSP70/HSC70 monoclonal IgG1, clonality 5G1-95
• Alternative technique
  murine, antibodies
• Clone name
  5G1-95

Gene info

• Identity
  HGNC:5233
• Gene
  HSPA1B
• Long gene name
  heat shock protein family A (Hsp70) member 1B
• Synonyms gene name
  • heat shock 70kD protein 1B
  • heat shock 70kDa protein 1B
• Synonyms
  • HSP70-2
• Locus
  6p21.33
• Discovery year
  1995-08-11
• Entrez gene record
  3304
• Pubmed identfication
  • 1700760
• Classification
  • TIM23 complex
  • Heat shock 70kDa proteins
• VEGA ID
  OTTHUMG00000031202

Gene info

• Identity
  HGNC:5232
• Gene
  HSPA1A
• Long gene name
  heat shock protein family A (Hsp70) member 1A
• Synonyms gene
  • HSPA1
• Synonyms gene name
  • heat shock 70kD protein 1A
  • heat shock 70kDa protein 1A
• Synonyms
  • HSP70-1
• GenBank acession
  • BC002453
• Locus
  6p21.33
• Discovery year
  2001-06-22
• Entrez gene record
  3303
• Classification
  • TIM23 complex
  • Heat shock 70kDa proteins
• VEGA ID
  OTTHUMG00000031201

Gene info

• Identity
  HGNC:29526
• Gene
  HSPA14
• Long gene name
  heat shock protein family A (Hsp70) member 14
• Synonyms gene name
  • heat shock 70kDa protein 14
• Synonyms
  • HSP70-4
  • HSP70L1
• GenBank acession
  • AF112210
• Locus
  10p13
• Discovery year
  2004-04-20
• Entrez gene record
  51182
• Pubmed identfication
  • 21208456
• RefSeq identity
  • NM_016299
• Classification
  • Heat shock 70kDa proteins

Gene info

• Identity
  HGNC:5234
• Gene
  HSPA1L
• Long gene name
  heat shock protein family A (Hsp70) member 1 like
• Synonyms gene name
  • heat shock 70kD protein-like 1
  • heat shock 70kDa protein 1-like
• Synonyms
  • HSP70-HOM
  • hum70t
• GenBank acession
  • D85730
• Locus
  6p21.33
• Discovery year
  1989-05-12
• Entrez gene record
  3305
• Pubmed identfication
  • 9685725
  • 9349405
• Classification
  • TIM23 complex
  • Heat shock 70kDa proteins
• VEGA ID
  OTTHUMG00000031208

Gene info

• Identity
  HGNC:5241
• Gene
  HSPA8
• Long gene name
  heat shock protein family A (Hsp70) member 8
• Synonyms gene
  • HSPA10
• Synonyms gene name
  • heat shock 70kD protein 8
  • heat shock 70kDa protein 8
• Synonyms
  • HSC71
  • HSC70
  • HSP73
• GenBank acession
  • Y00371
• Locus
  11q24.1
• Discovery year
  1995-05-08
• Entrez gene record
  3312
• Pubmed identfication
  • 8530083
  • 3037489
• Classification
  • Spliceosomal P complex
  • Heat shock 70kDa proteins
  • Spliceosomal C complex
• VEGA ID
  OTTHUMG00000166030

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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