Mouse anti P110 antibody (mitochondrial membrane protein)
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Catalog numberA115M
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PricePlease ask
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Size100 μg
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CategoryPrimary Antibodies
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Long descriptionP110 is a human mitochondrial protein with a molecular weight of 110 kDa. Close examination of the staining pattern in HeLa and Fanconi's Anemia cells reveal differences in the morphology and organization of mitochondria in these two cell types. The epitope targeted may serve as a valuable marker in the investigation of relationships between mitochondria and other cellular structures in human cells. The mouse monoclonal P110 antibody labels mitochondria in human cells, as assessed by double staining with either Rhodamine 123 or a polyclonal antibody to mitochondrial matrix HSP-60 proteins. The P110 antigen has an approximate isoelectric point of 6.5 that copartitions with HSP-60 proteins during isolation of mitochondria from HeLa cells. The P110 staining pattern in HeLa and Fanconi's anaemia cells reveals differences in the morphology and organization of mitochondria in these two cell types. In HeLa cells, mitochondria appear as individual tubular compartments of variable length and are closely associated with vimentin filaments, particularly at the periphery of the nucleus. In Fanconi's anaemia cells, mitochondria have a filamentous shape and form an interconnected cytoplasmic reticulum running in parallel with both vimentin filaments and microtubules. After stabilization with aldehyde- or alcohol-based fixation protocols that optimize the preservation of cytoskeletal components, the epitope targeted by the 2G2 antibody may serve as a valuable marker in the investigation of relationships between mitochondria and other cellular structures in human cells.
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Antibody come fromSkeletal fracrtion of HeLa-S3 cells
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Other descriptionProvided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
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Clone2G2
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Antigen antibody binding interactionMouse anti P110 antibody (mitochondrial membrane protein) Antibody
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Antibody is raised inMouse
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Antibody s reacts withHuman, Monkey
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Antibody s reacts with these speciesThis antibody doesn't cross react with other species
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Antibody s specificityNo Data Available
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ApplicationWestern Blot, Immunofluorescense
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Antibody s suited forApplication: Western Blot at 2-10 ug/ml. Immunofluorescence staining.
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Storage-20ºC
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Relevant references1. Paulin-Levasseur M; Chen G; Larivi`ere C. The 2G2 antibody recognizes an acidic 110-kDa human mitochondrial protein, Histochem J, 30(9):617-25 1998.
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Protein numbersee ncbi
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WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Associated membrane protein types are lipopolysaccharide selective barriers. Biological membranes include cell membranes, outer coverings of cells or organelles that allow passage of certain proteins and nuclear membranes, which cover a cell nucleus; and tissue membranes, such as mucosae and serosae.
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PropertiesIf you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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French translationanticorps
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Gene target
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Gene symbolEIF3C, SART3, CDK11B, CDK11A
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Short nameMouse anti P110 antibody (mitochondrial protein)
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TechniqueAntibody, Mouse, anti, antibody to, antibodies against human proteins, antibodies for, mouses
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Hostmouse
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IsotypeIgG1
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Labelunlabeled
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SpeciesMouse, Mouses
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Alternative nameMouse antibody to P110 (antibody to-) (mitochondrial membrane protein)
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Alternative techniqueantibodies, murine
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Tissuemitochondrial
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Gene info
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Identity
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Gene
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Long gene nameeukaryotic translation initiation factor 3 subunit C
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Synonyms gene
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Synonyms gene name
- eukaryotic translation initiation factor 3, subunit 8, 110kDa
- eukaryotic translation initiation factor 3, subunit C
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-11-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Eukaryotic translation initiation factor 3
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namespliceosome associated factor 3, U4/U6 recycling protein
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Synonyms gene name
- squamous cell carcinoma antigen recognized by T-cells 3
- squamous cell carcinoma antigen recognized by T cells 3
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-12-17
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Entrez gene record
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Pubmed identfication
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Classification
- RNA binding motif containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namecyclin dependent kinase 11B
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Synonyms gene
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Synonyms gene name
- cell division cycle 2-like 1 (PITSLRE proteins)
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Synonyms
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GenBank acession
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Locus
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Discovery year1991-07-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Cyclin dependent kinases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecyclin dependent kinase 11A
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Synonyms gene
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Synonyms gene name
- cell division cycle 2-like 2
- cell division cycle 2-like 2 (PITSLRE proteins)
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Synonyms
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GenBank acession
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Locus
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Discovery year1991-07-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Cyclin dependent kinases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
