Mouse anti Nestin

  • Catalog number
    MUB1302P
  • Price
    Please ask
  • Size
    0,1 mg
  • Category
    Primary Antibodies
  • Long description
    Nestin is a class IV intermediate filament protein abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. This cytoskeletal protein has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. This highly specific antibody to human nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells.
  • Antibody come from
    Monoclonal antibody 10C2 was derived by fusion of P3x63Ag8.653 myeloma cells with spleen cells from a BALB/c mouse immunized with a 150-amino-acid fragment from the cloned human nestin, conjugated to glutathione S-transferase.
  • Other description
    Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
  • Clone
    10C2
  • Antigen antibody binding interaction
    Mouse anti Nestin Antibody
  • Antibody is raised in
    Mouse
  • Antibody s reacts with
    Human
  • Antibody s reacts with these species
    This antibody doesn't cross react with other species
  • Antibody s specificity
    RNF406 is suitable for immunoblotting and immunohistochemistry on frozen and paraffin-embedded tissues.
  • Research interest
    Cardiovascular,Cytoskeleton,Neurobiology,Stem cell research
  • Application
    Immunocytochemistry,Immunohistochemistry (frozen),Immunohistochemistry (paraffin),Western blotting
  • Antibody s suited for
    The 10C2 nestin antibody is useful for immunocytochemistry on fixed cells, immunohistochemistry on frozen and paraffin-embedded tissues, and Western blotting. Optimal antibody dilution should be determined by titration; recommended range is 1:100 to 1:200 for immunocytochemistry and immunohistochemistry with a fluorescein or rhodamine conjugated anti-mouse secondary antibody, and 1:1000 to 1:5000 for Western blot applications using chemoluminescent detection.
  • Storage
    Can be stored at -20o C to -70o C for long term storage. Once thawed, store at 4oC, do not refreeze.
  • Relevant references
    1. Messam, C., Hou, J., Major, E.O. (2000). Co-expression of nestin in neural and glial cells in the developing human CNS defined by a human specific anti-nestin antibody. Exp Neurol 161, 585-96. 2. Messam, C., Hou, J., Berman, J.W., Major, E.O. (2002) Analysis of the temporal expression of nestin in human fetal brain derived neuronal and glial progenitor cells. Brain Res Dev Brain Res 134, 87-92. 3. Hou, Y.Y., et al. (2006). Schwannoma of the gastrointestinal tract: a clinicopathological, immunohistochemical and ultrastructural study of 33 cases. Histopathology 48, 536-45. 4. Mokry, J., Pudil, R., Ehrmann, J., Cizkova, D., Osterreicher, J., Filip, S., Kolar, Z. (2008). Re-expression of nestin in the myocardium of postinfarcted patients. Virchows Arch 453, 33-41. 5. Corsino, P.E., et al. (2008). Mammary tumors initiated by constitutive Cdk2 activation contain an invasive basal-like component. Neoplasia 10, 1240-52. 6. Murphy, A.J., Viero, S., Ho, M., Thorner, P.S. (2009). Diagnostic utility of nestin expression in pediatric tumors in the region of the kidney. Appl Immunohistochem Mol Morphol 17, 517-23. 7. Sellheyer, K., Krahl, D. (2010). Spatiotemporal expression pattern of neuroepithelial stem cell marker nestin suggests a role in dermal homeostasis, neovasculogenesis, and tumor stroma development: a study on embryonic and adult human skin. J Am Acad Dermatol 63, 93-113. 8. Elamin, M.H., et al. (2010). Curcumin inhibits the Sonic Hedgehog signaling pathway and triggers apoptosis in medulloblastoma cells. Mol. Carcinog. 49, 302-14. 9. Popescu, L.M., et al. (2010). Telocytes in human epicardium. J Cell Mol Med 14, 2085-93. 10. Sellheyer, K., Krahl, D. (2011). Does the peritumoral stroma of basal cell carcinoma recapitulate the follicular connective tissue sheath? J Cutan Pathol 38, 551-9.
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
    Nestin  
  • Gene symbol
    NES
  • Short name
    Mouse anti Nestin
  • Technique
    Mouse, anti, antibody to, mouses
  • Host
    mouse
  • Isotype
    IgG1
  • Label
    unlabeled
  • Species
    Mouse, Mouses
  • Alternative name
    Mouse antibody to Nestin
  • Alternative technique
    murine, antibodies
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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