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Clone
5D6
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Immunogen
A synthetic peptide EQKLISEEDL coupled to KLH.
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Antibody s full description
Mouse Anti-Human myelocytomatosis tag Monoclonal IgG1 Antibody, Clone: 5D6: ATTO 633
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
avian myelocytomatosis viral oncogene homolog Antibody, c-Myc Antibody, MRTL Antibody, MYC Antibody, Myc Epitope Tag Antibody, oncogene c-Myc Antibody, proto-oncogene c-Myc Antibody
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Raised in
Mouse
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Antibody s target
myelocytomatosis tag
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Brandname
None
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Antibodies applications
WB, IP, ICC/IF
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Antibody s reactivity
Species Independent
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Antibody s dilutions
WB (1:10000), IP (1:200), ICC/IF (1:1000); optimal dilutions for assays should be determined by the user.
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Antibody buffer for storage
PBS (without Mg2+ and Ca2+), 50% glycerol, pH 7.4, 150mM NaCl, 0.02% sodium azide
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Antibody s specificity
Detect C-Myc fusion proteins.
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
A 1:10000 dilution of SMC-255 was sufficient for detection of Myc Tag in 10 µg of Recombinant Protein by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
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Antibody in cell
See included datasheet or contact our support service
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems.
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Bibliography
1. Munro S. and Pelham H.R. (1984) EMBO J. 3: 3087-93.
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Released date
06-Sep-2016
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
Refer to NCBI
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Gene number
Refer to GenBank
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Protein number
Refer to Swiss-Prot
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Antibody s datasheet
Contact our support service
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Representative figure legend
Western Blot analysis of Recombinant Protein showing detection of ~41 kDa Myc Tag protein using Mouse Anti-Myc Tag Monoclonal Antibody, Clone 5D6 (SMC-255). Lane 1: 1:5,000 Dilution. Lane 2: 1:10,000 Dilution. Primary Antibody: Mouse Anti-Myc Tag Monoclonal Antibody (SMC-255). Predicted/Observed Size: ~41 kDa. Mouse Anti-Myc Tag Antibody [5D6] used in Western Blot (WB) on Recombinant Protein (SMC-255)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Gene target
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Short name
Mouse Anti-Myc Tag Monoclonal IgG1 , Clone 5D6
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Technique
anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses, Tags
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Host
mouse
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Isotype
IgG1 , IgG1
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Label
ATTO 633
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Species
Mouses
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Alternative name
Mouse Antibody toMyc detection labelled monoclonal IgG1 , clonality 5D6
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Alternative technique
murine, antibodies
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Alternative to gene target
v-myc myelocytomatosis viral oncogene homolog (avian), bHLHe39 and c-Myc and MRTL and MYCC, MYC and IDBG-35442 and ENSG00000136997 and 4609, E-box binding, nuclei, Bmyc and IDBG-147082 and ENSMUSG00000049086 and 107771, MYC and IDBG-644147 and ENSBTAG00000008409 and 511077
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Clone name
5D6
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