Mouse anti mTOR

• Catalog number
  X1974M
• Price
  Please ask
• Size
  100 μg

• Category
  Primary Antibodies
• Long description
  Mammalian target of Rapamycin (mTOR), also known as FKBP12-rapamycin-associated protein (FRAP) is a ~280 kDa serine/threonine kinase and a key modulator of cell growth and protein synthesis. mTOR is implicated in cancer and neurite plasticity, and plays a central role in mediating phosphoinositide 3-kinase (PI3 kinase) and Akt/PKB signals for cell growth, proliferation, and protein translation via ribosomal S6 kinase (S6Ks) and translation regulator eIF4E-binding protein 1. mTOR is differentially phosphorylated on threonine 2446 and serine 2448 in response to nutrient status and growth factor stimulation.
• Antibody come from
  The antiserum was produced against a synthetic peptide (KLH-coupled) corresponding to residues near the carboxy-terminal sequence of human VEGF Receptor 2.
• Other description
  Provided as solution in phosphate buffered saline, pH 7.3, 50% glycerol with 1.0 mg/ml BSA and 0.05% sodium azide. Protein A/G Chromatography
• Clone
  215Q18
• Antigen antibody binding interaction
  Mouse anti mTOR Antibody
• Antibody is raised in
  Mouse
• Antibody s reacts with
  Human, Mouse, Rat
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  No Data Available
• Application
  Western Blot
• Antibody s suited for
  The antibody has been used in Western blotting and immunoprecipitation. Other applications may work but have not been tested._x000B_For Western blotting applications, we recommend using the antibody at a 1:1000 starting dilution. The optimal antibody concentration should be determined empirically for each specific application.
• Storage
  -20ºC
• Relevant references
  1. Homqvist, K. (2004) The adaptor protein shb binds to tyrosine 1175 in vascular endothelial growth factor (VEGF) receptor-2 and regulates VEGF-dependent cellular migration. J. Biol. Chem. 279(21):22267-22275._x000B_2. Meyer, R.D., et al. (2002) The presence of a single tyrosine residue at the carboxyl domain of vascular endothelial growth factor receptor-2/FLK-1 regulates its autophosphorylation and activation of signaling molecules. J. Biol. Chem. 277(30):27081-27087._x000B_3. Zeng, H., et al. (2001) Tyrosine residues 951 and 1059 of vascular endothelial growth factor receptor-2 (KDR) are essential for vascular permeability factor/vascular endothelial growth factor- inducedendotheliummigrationandproliferation,respectively.J.Biol.Chem. 276(35):32714-32719._x000B_4. Rahimi, N., et al. (2000) Receptor chimeras indicate that the vascular endotheilial growth factor receptor-1 (VEGFR-1) modulates mitogenic activity of VEGFR-2 in endothelial cells. J. Biol. Chem. 275(22):16986-16992._x000B_5. Sato, Y., et al. (2000) Properties of two VEGF receptors, Flt-1 and KDR, in signal transduction. Ann. N.Y. Acad. Sci. 902:201-207._x000B_6. Rahimi, N. and A. Kazlauskas (1999) A role for cadherin-5 in regulation of vascular endothelial growth factor receptor 2 activity in endothelial cells. Mol. Biol. Cell 10(10):3401-3407.
• Protein number
  see ncbi
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  mTOR  
• Gene symbol
  MTOR-AS1, RPTOR, RICTOR
• Short name
  Mouse anti mTOR
• Technique
  Mouse, anti, antibody to, mouses
• Host
  mouse
• Isotype
  IgG1
• Label
  unlabeled
• Species
  Mouse, Mouses
• Alternative name
  Mouse antibody to Mammalian target on rapamycin
• Alternative technique
  murine, antibodies
• Alternative to gene target
  mechanistic target of rapamycin (serine/threonine kinase), FRAP and FRAP1 and FRAP2 and RAFT1 and RAPT1, MTOR and IDBG-89258 and ENSG00000198793 and 2475, transferase activity, nuclei, Mtor and IDBG-204403 and ENSMUSG00000028991 and 56717, MTOR and IDBG-632731 and ENSBTAG00000015325 and 100139219

Gene info

• Identity
  HGNC:40242
• Gene
  MTOR-AS1
• Long gene name
  MTOR antisense RNA 1
• Synonyms gene name
  • MTOR antisense RNA 1 (non-protein coding)
• Locus
  1p36.22
• Discovery year
  2011-05-18
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000002003

Gene info

• Identity
  HGNC:30287
• Gene
  RPTOR
• Long gene name
  regulatory associated protein of MTOR complex 1
• Synonyms gene name
  • regulatory associated protein of MTOR, complex 1
• Synonyms
  • KOG1
  • Mip1
  • KIAA1303
  • raptor
• Synonyms name
  • regulatory associated protein of mTOR
• Locus
  17q25.3
• Discovery year
  2009-05-29
• Entrez gene record
  57521
• Pubmed identfication
  • 10718198
  • 12150926
• RefSeq identity
  • NM_020761
• Classification
  • WD repeat domain containing
  • MTOR complex 1
  • Armadillo like helical domain containing
• VEGA ID
  OTTHUMG00000177622

Gene info

• Identity
  HGNC:28611
• Gene
  RICTOR
• Long gene name
  RPTOR independent companion of MTOR complex 2
• Synonyms gene name
  • RPTOR independent companion of MTOR, complex 2
• Synonyms
  • MGC39830
  • AVO3
  • PIA
  • KIAA1999
• Synonyms name
  • rapamycin-insensitive companion of mTOR
  • pianissimo
• Locus
  5p13.1
• Discovery year
  2009-05-29
• Entrez gene record
  253260
• Pubmed identfication
  • 15268862
• RefSeq identity
  • NM_152756
• Classification
  • MTOR complex 2
  • Armadillo like helical domain containing
• VEGA ID
  OTTHUMG00000162037

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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