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Clone
S277-7
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Immunogen
Fusion protein amino acids 168-255 (Cytoplasmic C2A domain) of mouse Synaptotagmin-12
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Antibody s full description
Mouse Anti-Mouse Synaptotagmin 12 Monoclonal IgG1 Antibody, Clone: S277-7: ATTO 680
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
SYT-12 Antibody, SYT12 Antibody, Synaptotagmin 12 Antibody, Synaptotagmin XII Antibody, SytXII Antibody, Syt XII Antibody, Synaptotagmin12 Antibody, SynaptotagminXII Antibody
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Raised in
Mouse
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Antibody s target
Synaptotagmin 12
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Primary research fields
Neuroscience, Cell Structure, Pre-Synaptic Markers
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Brandname
None
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Antibodies applications
WB, IHC, ICC/IF
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s dilutions
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~45kDa. Does not cross-react with Synaptotagmin-6 or other Synaptotagmins.
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
1 µg/ml of SMC-437 was sufficient for detection of Synaptotagmin-12 in 20 µg of transiently overexpressing synaptotagmin-12 COS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasmic Vesicle
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
Synaptotagmins constitute a family of membrane trafficking proteins that are characterized by an N-terminal transmembrane region (TMR), a variable linker, and two C-terminal C2 domains - C2A and C2B. There are 15 members in the mammalian synaptotagmin family. There are several C2-domain containing protein families that are related to synaptotagmins, including transmembrane (Ferlins, E-Syts, and MCTPs) and soluble (RIMs, Munc13s, synaptotagmin-related proteins and B/K) proteins. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin participates in triggering neurotransmitter release at the synapse. The first domain mediates Ca(2+)-dependent phospholipid binding. The second C2 domain mediates interaction with Stonin 2. Synaptotagmin may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2 (1, 2).
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Bibliography
1. Schengrund C.L., et al. (2002) J Biol Chem. 277: 32815. 2. Reichardt L.F., et al. (1981) J Cell Biol. 91:257.
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Released date
1-Mar-2013
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
NP_598925.1
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Gene number
171180
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Protein number
Q920N7
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Antibody s datasheet
Contact our support service
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Synaptotagmin-12 Monoclonal Antibody, Clone S277-7 (SMC-437). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Synaptotagmin-12 Monoclonal Antibody (SMC-437) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasmic Vesicle, Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Synaptotagmin-12 Antibody (D) Composite. Western Blot analysis of Monkey COS cells transfected with GFP-tagged Synaptotagmin showing detection of ~45 kDa Synaptotagmin-12 protein using Mouse Anti-Synaptotagmin-12 Monoclonal Antibody, Clone S277-7 (SMC-437). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged Synaptotagmin. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Synaptotagmin-12 Monoclonal Antibody (SMC-437) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~45 kDa. Mouse Anti-Synaptotagmin-12 Antibody [S277-7] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-437) Mouse Anti-Synaptotagmin-12 Antibody [S277-7] used in Western Blot (WB) on Monkey COS cells transfected with GFP-tagged Synaptotagmin (SMC-437)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Gene target
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Gene symbol
PIRC34, PIRC33, PIRC32, SNORD114-7, IGHV3OR15-7, SNORD115-7, RNU6-7, PIRC29, PIRC30, PIRC31
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Short name
Mouse Anti-Mouse Synaptotagmin-12 Monoclonal IgG1, Clone S277-7
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Technique
anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Host
mouse
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Isotype
IgG1, IgG1
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Label
ATTO 680
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Species
Mouse, Mouses
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Alternative name
Mouse Antibody toMouse Synaptotagmin-12 monoclonal IgG1, clonality S277-7
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Alternative technique
murine, antibodies
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Clone name
S277-7
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