Mouse Anti-Mouse REEP2 Monoclonal IgG2A, Clone S326D-2
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Catalog numberSMC-481D
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PricePlease ask
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Size100 µg
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CloneS326D-2
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ImmunogenFusion protein amino acids 111-254 (cytoplasmic C-terminus) of mouse REEP2
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Antibody s full descriptionMouse Anti-Mouse REEP2 Monoclonal IgG2A Antibody, Clone: S326D-2: Unconjugated
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameReceptor expression-enhancing protein 2 Antibody, C2orf19 Antibody, SGC32445 Antibody, LOC682105 Antibody
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Raised inMouse
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Antibody s targetREEP2
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Primary research fieldsNeuroscience, Neurodegeneration, Spastic Paraplegia, Cell Structure, Membrane Markers
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.1% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~30kDa.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysisA 1:100 dilution of SMC-481 was sufficient for detection of REEP2 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellMitochondrion , Mitochondrion Membrane , Endoplasmic Reticulum , Endoplasmic Reticulum Membrane
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextREEP2 belongs to the REEP family, which are transmembrane proteins that interact with odorant receptor proteins and may enhance the odorant receptor responses to odorants. REEP2 is also an integral membrane protein expressed in taste cells. Unlike its counterpart REEP1, REEP2 does not increase cell surface expression to sweet receptors, but instead alters their spatial organization (1).
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Bibliography1. Ilegems E., et al. (2010) J Neurosci. 30(41): 13774-13783.
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Released date10-Mar-2015
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_001191843.1
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Gene number225362
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Protein numberQ8VCD6
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-REEP2 Monoclonal Antibody, Clone S326D-2 (SMC-481). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-REEP2 Monoclonal Antibody (SMC-481) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Mitochondrion, Mitochondrion Membrane, Endoplasmic Reticulum, Endoplasmic Reticulum Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) REEP2 Antibody (D) Composite. Western Blot analysis of COS-REEP2-His showing detection of ~ 30 kDa REEP2 protein using Mouse Anti-REEP2 Monoclonal Antibody, Clone S326D-2 (SMC-481). Lane 1: MW Ladder. Lane 2: COS-REEP1-His. Lane 3: COS-REEP2-His. Lane 4: COS-REEP6-His. Load: 15 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-REEP2 Monoclonal Antibody (SMC-481) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~ 30 kDa. Mouse Anti-REEP2 Antibody [S326D-2] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-481) Mouse Anti-REEP2 Antibody [S326D-2] used in Western Blot (WB) on COS-REEP2-His (SMC-481)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolREEP2, MIR1289-2, MIR521-2, MIR509-2, MIR512-2, MIR7-2, MIR329-2, RNU6-2, KRTAP13-2, SNORD114-2
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Short nameMouse Anti-Mouse REEP2 Monoclonal IgG2A, Clone S326D-2
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG2A, IgG2a
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LabelUnconjugated
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SpeciesMouse, Mouses
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Alternative nameMouse Antibody toMouse REEP2 monoclonal IgG2A, clonality S326D-2
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Alternative techniquemurine, antibodies
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Clone nameS326D-2
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Gene info
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Identity
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Gene
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Long gene namereceptor accessory protein 2
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Synonyms gene
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Synonyms gene name
- chromosome 5 open reading frame 19
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-04-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Receptor accessory proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1289-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1289 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 521-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year2005-07-15
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR515 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 509-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year2007-10-24
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR506 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 512-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year2005-07-15
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR506 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 7-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR7 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 329-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year2005-07-11
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR329 family
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 2
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Synonyms
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Locus
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Discovery year2008-06-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nuclear RNAs
Gene info
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Identity
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Gene
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Long gene namekeratin associated protein 13-2
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-02-21
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Entrez gene record
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Pubmed identfication
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Classification
- Keratin associated proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 114-2
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Synonyms
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Locus
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Discovery year2006-09-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data