Mouse Anti-Mouse REEP2 Monoclonal IgG2A, Clone S326D-2

  • Catalog number
    SMC-481D
  • Price
    Please ask
  • Size
    100 µg
  • Clone
    S326D-2
  • Immunogen
    Fusion protein amino acids 111-254 (cytoplasmic C-terminus) of mouse REEP2
  • Antibody s full description
    Mouse Anti-Mouse REEP2 Monoclonal IgG2A Antibody, Clone: S326D-2: Unconjugated
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    Receptor expression-enhancing protein 2 Antibody, C2orf19 Antibody, SGC32445 Antibody, LOC682105 Antibody
  • Raised in
    Mouse
  • Antibody s target
    REEP2
  • Primary research fields
    Neuroscience, Neurodegeneration, Spastic Paraplegia, Cell Structure, Membrane Markers
  • Brandname
    None
  • Antibodies applications
    WB, IHC, ICC/IF
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s dilutions
    WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH7.4, 50% glycerol, 0.1% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~30kDa.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    A 1:100 dilution of SMC-481 was sufficient for detection of REEP2 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    Mitochondrion , Mitochondrion Membrane , Endoplasmic Reticulum , Endoplasmic Reticulum Membrane
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    REEP2 belongs to the REEP family, which are transmembrane proteins that interact with odorant receptor proteins and may enhance the odorant receptor responses to odorants. REEP2 is also an integral membrane protein expressed in taste cells. Unlike its counterpart REEP1, REEP2 does not increase cell surface expression to sweet receptors, but instead alters their spatial organization (1).
  • Bibliography
    1. Ilegems E., et al. (2010) J Neurosci. 30(41): 13774-13783.
  • Released date
    10-Mar-2015
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    NP_001191843.1
  • Gene number
    225362
  • Protein number
    Q8VCD6
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-REEP2 Monoclonal Antibody, Clone S326D-2 (SMC-481). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-REEP2 Monoclonal Antibody (SMC-481) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Mitochondrion, Mitochondrion Membrane, Endoplasmic Reticulum, Endoplasmic Reticulum Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) REEP2 Antibody (D) Composite. Western Blot analysis of COS-REEP2-His showing detection of ~ 30 kDa REEP2 protein using Mouse Anti-REEP2 Monoclonal Antibody, Clone S326D-2 (SMC-481). Lane 1: MW Ladder. Lane 2: COS-REEP1-His. Lane 3: COS-REEP2-His. Lane 4: COS-REEP6-His. Load: 15 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-REEP2 Monoclonal Antibody (SMC-481) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~ 30 kDa. Mouse Anti-REEP2 Antibody [S326D-2] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-481) Mouse Anti-REEP2 Antibody [S326D-2] used in Western Blot (WB) on COS-REEP2-His (SMC-481)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
  • Gene symbol
    REEP2, MIR1289-2, MIR521-2, MIR509-2, MIR512-2, MIR7-2, MIR329-2, RNU6-2, KRTAP13-2, SNORD114-2
  • Short name
    Mouse Anti-Mouse REEP2 Monoclonal IgG2A, Clone S326D-2
  • Technique
    anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
  • Host
    mouse
  • Isotype
    IgG2A, IgG2a
  • Label
    Unconjugated
  • Species
    Mouse, Mouses
  • Alternative name
    Mouse Antibody toMouse REEP2 monoclonal IgG2A, clonality S326D-2
  • Alternative technique
    murine, antibodies
  • Clone name
    S326D-2
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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