-
Clone
S174B-27
-
Immunogen
Fusion protein amino acids 720-804 (variable cytoplasmic domain) of mouse Gamma- protocadherin-C3. Human: 97% identity (83/85 amino acids identical). Rat: 97% identity (83/85 amino acids identical). No significant identity with other Gamma-protocadherin proteins.
-
Antibody s full description
Mouse Anti-Mouse Protocadherin Gamma C3 Monoclonal IgG1 Antibody, Clone: S174B-27: HRP
-
Antibody s category
Monoclonal Antibodies
-
Antibody s other name
PCDHGC3 Antibody, Cadherin like 2 Antibody, PC 43 Antibody, PC43 Antibody, PCDH 2 Antibody, PCDH gamma C3 Antibody, PCDH2 Antibody, PCDHGC 3 Antibody, Protocadherin 2 Antibody, Protocadherin 43 Antibody, Protocadherin gamma C3 Antibody, Protocadherin gamma subfamily C 3 Antibody, Protocadherin2 Antibody, Protocadherin43 Antibody, Pcdhgb6 Antibody
-
Raised in
Mouse
-
Antibody s target
Protocadherin Gamma C3
-
Primary research fields
Neuroscience, Cell Structure, Membrane Markers
-
Brandname
None
-
Antibodies applications
WB, ICC/IF
-
Antibody s reactivity
Human, Mouse
-
Antibody s dilutions
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
-
Purity
Protein G Purified
-
Antibody buffer for storage
PBS pH 7.4, 50% glycerol, 0.1% sodium azide
-
Antibody s concentration
1 mg/ml
-
Antibody s specificity
Detects ~100kDa.
-
Storage recommendations
-20ºC
-
Shipping recommendations
Blue Ice or 4ºC
-
Antibody certificate of analysis
1 µg/ml of SMC-474 was sufficient for detection of Protocadherin Gamma C3 in 20 µg of COS cell lysate (transiently transfected with GFP-tagged Protocadherin Gamma C3 plasmids) by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
-
Antibody in cell
Cell Membrane
-
Tissue specificity
See included datasheet or contact our support service
-
Scientific context
This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. PCDHGC3 is a potential calcium-dependent cell-adhesion protein. It may be involved in the establishment and maintenance of specific neuronal connections in the brain. This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.
-
Released date
11-Dec-2013
-
Tested applications
To be tested
-
Tested reactivity
To be tested
-
NCBI number
NP_291059.1
-
Gene number
93706
-
Protein number
Q91XX1
-
Antibody s datasheet
Contact our support service
-
Representative figure link
-
Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Protocadherin Gamma C3 Monoclonal Antibody, Clone S174B-27 (SMC-474). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Protocadherin Gamma C3 Monoclonal Antibody (SMC-474) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Protocadherin Gamma C3 Antibody (D) Composite. Western Blot analysis of Monkey COS cells transfected with GFP-tagged Gamma-cadherin-C3 showing detection of ~100 kDa Protocadherin Gamma C3 protein using Mouse Anti-Protocadherin Gamma C3 Monoclonal Antibody, Clone S174B-27 (SMC-474). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged Gamma-cadherin-C3. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Protocadherin Gamma C3 Monoclonal Antibody (SMC-474) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~100 kDa. Mouse Anti-Protocadherin Gamma C3 Antibody [S174B-27] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-474) Mouse Anti-Protocadherin Gamma C3 Antibody [S174B-27] used in Western Blot (WB) on Monkey COS cells transfected with GFP-tagged Gamma-cadherin-C3 (SMC-474)
-
Warning information
Non-hazardous
-
Country of production
Canada
-
Total weight kg
1.4
-
Net weight g
0.1
-
Stock availability
In Stock
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus
-
Gene target
-
Gene symbol
SNORD115-27, IGKV1D-27, RNVU1-27, IGHV7-27, ERVK-27, ERVW-27, SNORD114-27, SNORD116-27, IGLV3-27, IGHD7-27
-
Short name
Mouse Anti-Mouse Protocadherin Gamma C3 Monoclonal IgG1, Clone S174B-27
-
Technique
anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
-
Host
mouse
-
Isotype
IgG1, IgG1
-
Label
HRP
-
Species
Mouse, Mouses
-
Alternative name
Mouse Antibody toMouse Protocadherin g complement component 3 monoclonal IgG1, clonality S174B-27
-
Alternative technique
murine, antibodies
-
Alternative to gene target
complement component 3, AHUS5 and ARMD9 and ASP and C3a and C3b and CPAMD1 and HEL-S-62p, C3 and IDBG-21798 and ENSG00000125730 and 718, C5L2 anaphylatoxin chemotactic receptor binding, Extracellular, C3 and IDBG-193788 and ENSMUSG00000024164 and 102642251,12266, BT.19562 and IDBG-643930 and ENSBTAG00000017280 and 280677
-
Clone name
S174B-27
-
