Mouse Anti-Mouse Piccolo Monoclonal IgG2b Kappa, Clone 6H9-B6

  • Catalog number
    SMC-188D-A390
  • Price
    Please ask
  • Size
    100 µg
  • Clone
    6H9-B6
  • Immunogen
    Full length protein, BC001304.1
  • Antibody s full description
    Mouse Anti-Mouse Piccolo Monoclonal IgG2b Kappa Antibody, Clone: 6H9-B6: ATTO 390
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    ACZ Antibody, Aczonin Antibody, PCLO Antibody, Pico Antibody, presynaptic cytomatrix protein Antibody
  • Raised in
    Mouse
  • Antibody s target
    Piccolo
  • Primary research fields
    Neuroscience, Cell Structure, Pre-Synaptic Markers
  • Brandname
    None
  • Antibodies applications
    WB, ICC/IF, ELISA
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s dilutions
    WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH7.4, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~550kDa. No cross-reactivity to Bassoon.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    1 µg/ml of SMC-188 was sufficient for detection of Piccolo in 20 µg of rat brain tissue lysate by colorimetric immunoblot analysis using goat IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cell Junction , Synapse
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains and C-terminal PDZ and C2 domains (1, 2). In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones) (3). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Piccolo has also been shown to interfere with clathrin medicated endocytosis by binding to the F-actin and dynamin binding protein Abp1 (2).
  • Bibliography
    1. Gerber S.H., Garcia J., Rizo J., Sudhof T.C. (2001) EMBO J. 20(7): 1605-1619. 2. Wang X., et al. (1999) J Cell Biol. 147(1): 151-162. 3. Dresbach T., et al. (2006) J Biol Chem. 281(9):6038-6047.
  • Released date
    4-Nov-2010
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    NP_001104266.1
  • Gene number
    26875
  • Protein number
    Q9QYX7
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Piccolo Monoclonal Antibody, Clone 6H9-B6 (SMC-188). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Piccolo Monoclonal Antibody (SMC-188) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Endoplasmic Reticulum, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Piccolo Antibody (D) Composite. Western Blot analysis of Rat and mouse brain lysates showing detection of Piccolo protein using Mouse Anti-Piccolo Monoclonal Antibody, Clone 6H9-B6 (SMC-188). Primary Antibody: Mouse Anti-Piccolo Monoclonal Antibody (SMC-188) at 1:1000. (1) Mouse brain lysate; (2) Rat brain lysate. Mouse Anti-Piccolo Antibody [6H9-B6] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-188) Mouse Anti-Piccolo Antibody [6H9-B6] used in Western Blot (WB) on Rat and mouse brain lysates (SMC-188)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
  • Gene symbol
    PCDHGB6, MAGEB6
  • Short name
    Mouse Anti-Mouse Piccolo Monoclonal IgG2b Kappa, Clone 6H9-B6
  • Technique
    anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
  • Host
    mouse
  • Isotype
    IgG2b Kappa, IgG2b
  • Label
    ATTO 390
  • Species
    Mouse, Mouses
  • Alternative name
    Mouse Antibody toMouse Piccolo monoclonal IgG2b Kappa, clonality 6H9-B6
  • Alternative technique
    murine, antibodies
  • Clone name
    6H9-B6
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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