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Clone
P2B11
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Immunogen
GST-tagged recombinant protein corresponding to mouse MDC1 at and around the N-terminus
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Antibody s full description
Mouse Anti-Mouse MDC1 Monoclonal IgG1 Antibody, Clone: P2B11: APC
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
Nuclear factor with BRCT domains1 Antibody, mediator of DNA damage checkpoint 1 Antibody
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Raised in
Mouse
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Antibody s target
MDC1
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Primary research fields
Cancer, Oxidative Stress, Cell Signaling, Epigenetics
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Brandname
None
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Antibodies applications
WB, ICC/IF
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Antibody s reactivity
Human, Mouse, Bovine, Chimpanzee
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Antibody s dilutions
WB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~184kDa. This antibody recognizes MDC1 at and around the N-terminus.
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
0.5 µg/ml of SMC-197 was sufficient for detection of MDC1 in 10 µg of HeLa cell lysate by ECL immunoblot analysis.
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Antibody in cell
Nucleus
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
MDC1, mediator of DNA damage checkpoint protein 1, plays a role in checkpoint mediated cell cycle arrest in response to DNA damage, within S phase and G2/M. It is also thought to act as a scaffold protein during recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage that are marked by phosphorylation of histone H2A.X on S139.
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Bibliography
1. Lou Z., et al. (2004) J Biol Chem. 279(45): 46359-46362. 2. Luo K., Yuan J, Lou Z. (2011) J Biol Chem. 286(32): 28192-28199. 3. Strauss C., Halevy T., Macarov M., Argaman L., and Goldbery M. (2011) DNA Repair (Amst.). 10(8): 806-814. 4. Wilson K.A., et al. (2011) Mol Cancer Res. 9(6): 766-781.
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Released date
30-Aug-2011
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
NP_001010833.2
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Gene number
240087
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Protein number
Q5PSV9
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Antibody s datasheet
Contact our support service
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11 (SMC-197). Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody (SMC-197) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X. Western Blot analysis of Human 293Trap cell lysates showing detection of 184 kDa MDC1 protein using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11 (SMC-197). Lane 1: MW ladder. Lane 2: 293Trap cell lysates. Load: 30 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody (SMC-197) at 1:1000 for 2 hours RT. Secondary Antibody: Goat Anti-Mouse HRP: IgG at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 184 kDa. Western Blot analysis of Mouse Cortex and Cerebellum showing detection of 184 kDa MDC1 protein using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11 (SMC-197). Lane 1: MW ladder. Lane 2: Mouse Cortex. Lane 3: Mouse Cerebellum. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody (SMC-197) at 1:1000 for 2 hours RT. Secondary Antibody: Goat Anti-Mouse at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 184 kDa. Mouse Anti-MDC1 Antibody [P2B11] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Mouse Fibroblast cell line (NIH 3T3) (SMC-197) Mouse Anti-MDC1 Antibody [P2B11] used in Western Blot (WB) on Human 293Trap cell lysates (SMC-197) Mouse Anti-MDC1 Antibody [P2B11] used in Western Blot (WB) on Mouse Cortex and Cerebellum (SMC-197)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Gene target
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Gene symbol
MDC1-AS1, MDC1
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Short name
Mouse Anti-Mouse MDC1 Monoclonal IgG1, Clone P2B11
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Technique
anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Host
mouse
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Isotype
IgG1, IgG1
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Label
APC
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Species
Mouse, Mouses
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Alternative name
Mouse Antibody toMouse mediator on Desoxyribonucleic acid-damage checkpoint 1 monoclonal IgG1, clonality P2B11
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Alternative technique
murine, antibodies
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Alternative to gene target
mediator of DNA-damage checkpoint 1, NFBD1, MDC1 and IDBG-76700 and ENSG00000137337 and 9656, FHA domain binding, nuclei, Mdc1 and IDBG-179187 and ENSMUSG00000061607 and 240087, MDC1 and IDBG-634528 and ENSBTAG00000025526 and 511168
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Clone name
P2B11
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