Mouse Anti-Mouse GABA-A Receptor Beta1 Monoclonal IgG1, Clone S96-55

  • Catalog number
    SMC-340D-PCP
  • Price
    Please ask
  • Size
    100 µg
  • Clone
    S96-55
  • Immunogen
    Fusion protein amino acids 327-450 of mouse GABA-A-R-Beta1
  • Antibody s full description
    Mouse Anti-Mouse GABA A Receptor Monoclonal IgG1 Antibody, Clone: S96-55: PerCP
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    AW061132 antibody, B230208N19Rik antibody, GABA(A) receptor beta 1 antibody, GABA(A) receptor subunit beta-1 antibody, GABA-A receptor beta-1 polypeptide antibody, Gabrb-1 antibody, GABRB1 antibody, Gamma aminobutyric acid (GABA) A receptor beta 1 antibody, Gamma Aminobutyric Acid A Receptor Beta 1 antibody, Gamma Aminobutyric Acid Receptor beta-1 antibody, Gamma-aminobutyric acid (GABA) A receptor subunit beta 1 antibody, Gamma-aminobutyric acid receptor subunit beta-1 antibody, GARB1 antibody, GBRB1_HUMAN antibody
  • Raised in
    Mouse
  • Antibody s target
    GABA A Receptor
  • Primary research fields
    Neuroscience, Neurotransmitter Receptors, GABA Receptors, GABAA Receptors
  • Brandname
    None
  • Antibodies applications
    WB, IHC, ICC/IF , AM
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s dilutions
    WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH7.4, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~55kDa. No cross-reactivity against GABA-A-R-Beta 2 or –Beta3.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    2 µg/ml of SMC-340 was sufficient for detection of Beta1 GABA receptor in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cell Membrane , Cell Junction , Synapse , Postsynaptic Cell Membrane
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    The GABA-A receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (1). GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (2).
  • Bibliography
    1. Bracamontes J.R. and Steinbach J.H. (2008) J Bio Chem. 283: 26128-26136. 2. Macdonald R.L., Olsen R.W. (1993) Annu Rev Neurosci. 17: 569-602.
  • Released date
    13-Apr-2010
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    NP_032095.1
  • Gene number
    14400
  • Protein number
    P50571
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S96-55 (SMC-340). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody (SMC-340) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Positive. Western Blot analysis of Human Cell line lysates showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S96-55 (SMC-340). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody (SMC-340) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Immunohistochemistry analysis using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S96-55 (SMC-340). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody (SMC-340) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dermal staining. Mouse Anti-GABA A Receptor Antibody [S96-55] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SMC-340) Mouse Anti-GABA A Receptor Antibody [S96-55] used in Western Blot (WB) on Human Cell line lysates (SMC-340) Mouse Anti-GABA A Receptor Antibody [S96-55] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-340)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
  • Gene symbol
    IGHV4-55, IGLV11-55, SNORD110, ABAT, HLA-A, RNU6-55P, NEK1, RCN2, IGHV5-10-1, CIDEA
  • Short name
    Mouse Anti-Mouse GABA-A Receptor Beta1 Monoclonal IgG1, Clone S96-55
  • Technique
    anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
  • Host
    mouse
  • Isotype
    IgG1, IgG1
  • Label
    PerCP
  • Species
    Mouse, Mouses
  • Alternative name
    Mouse Antibody toMouse GABA-A Receptor Beta1 monoclonal IgG1, clonality S96-55
  • Alternative technique
    murine, antibodies
  • Clone name
    S96-55
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin heavy variable 4-55 (pseudogene)
  • Synonyms gene name
    • immunoglobulin heavy variable 4-55
    • immunoglobulin heavy variable 4-55 pseudogene
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-04
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin heavy locus at 14q32.33
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin lambda variable 11-55 (non-functional)
  • Synonyms gene name
    • immunoglobulin lambda variable 11-55
  • GenBank acession
  • Locus
  • Discovery year
    2000-05-08
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin lambda locus at 22q11.2
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    small nucleolar RNA, C/D box 110
  • Synonyms
  • Locus
  • Discovery year
    2006-05-08
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Small nucleolar RNAs, C/D box
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    RNA, U6 small nuclear 55, pseudogene
  • Synonyms gene
  • Synonyms gene name
    • RNA, U6 small nuclear 55
  • Locus
  • Discovery year
    2011-08-08
  • Entrez gene record
  • RefSeq identity
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin heavy variable 5-10-1
  • Synonyms gene
  • Synonyms gene name
    • immunoglobulin heavy variable 5-A (provisional, gene/pseudogene)
    • immunoglobulin heavy variable 5-A (provisional)
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-04
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin heavy locus at 14q32.33
  • VEGA ID
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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