Mouse Anti-Mouse ASIC1 Monoclonal IgG1, Clone S271-44

• Catalog number
  SMC-427D-A565
• Price
  Please ask
• Size
  100 µg

• Clone
  S271-44
• Immunogen
  Fusion protein amino acids 460-526 (Cytoplasmic C-terminus) of mouse ASIC1
• Antibody s full description
  Mouse Anti-Mouse ASIC1 Monoclonal IgG1 Antibody, Clone: S271-44: ATTO 565
• Antibody s category
  Monoclonal Antibodies
• Antibody s other name
  ACCN2 Antibody, Acid-sensing (proton-gated) ion channel Antibody, Acid-sensing ion channel 1 Antibody, Amiloride-sensitive cation channel 2 neuronal Antibody, BNAC2 Antibody, Brain sodium channel 2 Antibody
• Raised in
  Mouse
• Antibody s target
  ASIC1
• Primary research fields
  Neuroscience, Ion Channels, Sodium Channels, Proton-Gated Sodium Channels
• Brandname
  None
• Antibodies applications
  WB, IHC, ICC/IF
• Antibody s reactivity
  Mouse, Rat
• Antibody s dilutions
  WB (1:1000); optimal dilutions for assays should be determined by the user.
• Purity
  Protein G Purified
• Antibody buffer for storage
  PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Antibody s concentration
  1 mg/ml
• Antibody s specificity
  Detects ~60kDa.
• Storage recommendations
  -20ºC
• Shipping recommendations
  Blue Ice or 4ºC
• Antibody certificate of analysis
  1 µg/ml of SMC-427 was sufficient for detection of ASIC1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Antibody in cell
  Cell Membrane
• Tissue specificity
  See included datasheet or contact our support service
• Scientific context
  Acid sensing ion channel ASIC1, also designated ACCN2, BNAC2 and ASIC1a, is present in brain as a 4.3-kb transcript with localization to rat dorsal root ganglia. Insitu hybridization of rat brain suggests that ASIC1 is most abundant in the main olfactory bulb, cerebral cortex, hippocampal formation, habenula, basolateral amygdaloid nuclei and cerebellum. ASIC1 and H+-gated currents may contribute to the development of fear and anxiety.
• Bibliography
  1. Garcia-Anoveros J., Derfler B., Neville-Golden J., Hyman B.T. and Corey D.P. (1997) Proc. Natl. Acad. Sci. USA 94: 1459-1464. 2. Waldmann R., Champigny G., Bassilana F., Heurteaux C. and Lazdunski M. (1997) Nature. 386: 173-177. 3. Grunder S., Geisler H.S., Rainier S. and Fink J.K. (2001) Eur. J. Hum. Genet. 9:672-676. 4. Chen C.C., Zimmer A., Sun W.H., Hall J., Brownstein M.J. and Zimmer A. (2002) Proc. Natl. Acad. Sci. USA 99: 8992-8997. 5. Wemmie J.A., et al. (2004) Proc. Natl. Acad. Sci. USA 101: 3621-3626 .
• Released date
  15-Feb-2013
• Tested applications
  To be tested
• Tested reactivity
  To be tested
• NCBI number
  NP_033727.1
• Gene number
  11419
• Protein number
  Q6NXK8
• Antibody s datasheet
  Contact our support service
• Representative figure link
  more | details
• Representative figure legend
  Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-ASIC1 Monoclonal Antibody, Clone S271-44 (SMC-427). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-ASIC1 Monoclonal Antibody (SMC-427) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ASIC1 Antibody (D) Composite. Western Blot analysis of Rat brain lysates showing detection of ASIC1 protein using Mouse Anti-ASIC1 Monoclonal Antibody, Clone S271-44 (SMC-427). Primary Antibody: Mouse Anti-ASIC1 Monoclonal Antibody (SMC-427) at 1:100, 1:250, 1:500, and 1:1000. Mouse Anti-ASIC1 Antibody [S271-44] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-427) Mouse Anti-ASIC1 Antibody [S271-44] used in Western Blot (WB) on Rat brain lysates (SMC-427)
• Warning information
  Non-hazardous
• Country of production
  Canada
• Total weight kg
  1.4
• Net weight g
  0.1
• Stock availability
  In Stock
• Other related products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  ASIC1   Monoclonal   IgG1   S271-44  
• Gene symbol
  IGHVIII-44, SNORD115-44, IGLV1-44, IGHVIV-44-1, IGHVII-44-2, ZNF141, ASIC1, RNU6-44P, CUZD1, SQOR
• Short name
  Mouse Anti-Mouse ASIC1 Monoclonal IgG1, Clone S271-44
• Technique
  anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
• Host
  mouse
• Isotype
  IgG1, IgG1
• Label
  ATTO 565
• Species
  Mouse, Mouses
• Alternative name
  Mouse Antibody toMouse ASIC1 monoclonal IgG1, clonality S271-44
• Alternative technique
  murine, antibodies
• Clone name
  S271-44

Gene info

• Identity
  HGNC:5700
• Gene
  IGHVIII-44
• Long gene name
  immunoglobulin heavy variable (III)-44 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (III)-44
  • immunoglobulin heavy variable (III)-44 pseudogene
• Synonyms
  • IGHV(III)-44
• GenBank acession
  • M99673
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28345
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152065

Gene info

• Identity
  HGNC:33063
• Gene
  SNORD115-44
• Long gene name
  small nucleolar RNA, C/D box 115-44
• Synonyms
  • HBII-52-44
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-15
• Entrez gene record
  100033818
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003359.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:5879
• Gene
  IGLV1-44
• Long gene name
  immunoglobulin lambda variable 1-44
• GenBank acession
  • Z73654
• Locus
  22q11.22
• Discovery year
  2000-05-08
• Entrez gene record
  28823
• RefSeq identity
  • NG_000002
• Classification
  • Immunoglobulin lambda locus at 22q11.2
• VEGA ID
  OTTHUMG00000151050

Gene info

• Identity
  HGNC:5710
• Gene
  IGHVIV-44-1
• Long gene name
  immunoglobulin heavy variable (IV)-44-1 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (IV)-44-1
  • immunoglobulin heavy variable (IV)-44-1 pseudogene
• Synonyms
  • IGHV(IV)-44-1
• GenBank acession
  • AB019438
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28337
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152064

Gene info

• Identity
  HGNC:5681
• Gene
  IGHVII-44-2
• Long gene name
  immunoglobulin heavy variable (II)-44-2 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (II)-44-2
  • immunoglobulin heavy variable (II)-44-2 pseudogene
• Synonyms
  • IGHV(II)-44-2
• GenBank acession
  • AB019438
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28366
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152080

Gene info

• Identity
  HGNC:12926
• Gene
  ZNF141
• Long gene name
  zinc finger protein 141
• Synonyms gene
  • D4S90
• Synonyms gene name
  • zinc finger protein 141 (clone pHZ-44)
• Synonyms
  • pHZ-44
• GenBank acession
  • L15309
• Locus
  4p16.3
• Discovery year
  1993-02-11
• Entrez gene record
  7700
• Pubmed identfication
  • 8268908
• RefSeq identity
  • NM_003441
• Classification
  • Zinc fingers C2H2-type
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000159852

Gene info

• Identity
  HGNC:100
• Gene
  ASIC1
• Long gene name
  acid sensing ion channel subunit 1
• Synonyms gene
  • ACCN2
• Synonyms gene name
  • amiloride-sensitive cation channel 2, neuronal
  • acid-sensing (proton-gated) ion channel 1
  • acid sensing (proton gated) ion channel 1
• Synonyms
  • BNaC2
  • hBNaC2
• GenBank acession
  • U78181
• Locus
  12q13.12
• Discovery year
  1997-09-05
• Entrez gene record
  41
• Pubmed identfication
  • 9037075
• RefSeq identity
  • NM_020039
• Classification
  • Acid sensing ion channel subunits
• VEGA ID
  OTTHUMG00000169812

Gene info

• Identity
  HGNC:34288
• Gene
  RNU6-44P
• Long gene name
  RNA, U6 small nuclear 44, pseudogene
• Synonyms gene
  • RNU6-44
• Synonyms gene name
  • RNA, U6 small nuclear 44
• Locus
  11q23.1
• Discovery year
  2008-06-10
• Entrez gene record
  106478922

Gene info

• Identity
  HGNC:17937
• Gene
  CUZD1
• Long gene name
  CUB and zona pellucida like domains 1
• Synonyms gene name
  • CUB and zona pellucida-like domains 1
• Synonyms
  • ERG-1
  • UO-44
• GenBank acession
  • AF305835
• Locus
  10q26.13
• Discovery year
  2003-11-18
• Entrez gene record
  50624
• Pubmed identfication
  • 10542259
• RefSeq identity
  • NM_022034
• VEGA ID
  OTTHUMG00000019195

Gene info

• Identity
  HGNC:20390
• Gene
  SQOR
• Long gene name
  sulfide quinone oxidoreductase
• Synonyms gene
  • SQRDL
• Synonyms gene name
  • sulfide quinone reductase-like (yeast)
• Synonyms
  • CGI-44
  • SQR
• GenBank acession
  • AF042284
• Locus
  15q21.1
• Discovery year
  2003-01-31
• Entrez gene record
  58472
• Pubmed identfication
  • 10810093
  • 10224084
• VEGA ID
  OTTHUMG00000131476

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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