Mouse Anti-mCherry Polyclonal: ATTO 700

  • Catalog number
    SPC-1268D-A700
  • Price
    Please ask
  • Size
    100 µl
  • Immunogen
    Full-length protein.
  • Antibody s target
    Anaplasma marginale mCherry
  • Antibody s full description
    Mouse Anti-mCherry Polyclonal
  • Primary research fields
    Tags and Cell Markers, Fluorescent Proteins
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    mCherry Antibody, mcherry Antibody
  • Verified applications
    WB, IHC, ICC/IF, IP
  • Raised in
    Mouse
  • Antibody s reactivity
    Species Independent
  • Antibody s recommended dilutions for use
    WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Peptide Affinity Purified
  • Recommended buffer for storage
    PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
  • Antibody s concentration
    See included datasheet or contact our support service
  • Antibody s specificity
    The mCherry tag antibody can recognize mCherry and mCherry tag fusion proteins.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    A 1:10000 dilution of SPC-1268 was sufficient for detection of mCherry in 10 µg of mCherry Recombinant Protein by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    Contact our support service.
  • Tissue specificity
    See included datasheet or contact our support service.
  • Scientific context
    mCherry is a fluorescent tracer in transfection and transgenic experiments. Originally it was derived from proteins isolated from Cnidarians (jelly fish, sea anemones and corals), specicially DsRed, a red fluorescent protein from the disc corals of Discosoma species. DsRed is similar in properties to GFP, but, produces a red rather than a green fluoresence. mCherry was engineered with further multiple cycles of mutation, directed modification and evolutionary selection, and in its monomeric form has an excitation maximum at 587 nm and an emission maximum at 610 nm.
  • Bibliography
    1. Shaner N.C., et al. (2004). Nature Biotechnology. 22(12): 1567–1572.
  • Released date
    6/Sep/2016
  • NCBI number
    Refer to NCBI
  • Gene number
    Refer to GenBank
  • Protein number
    X5DSL3
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure legend
    Western blot analysis of mCherry Recombinant Protein showing detection of ~28kDa mCherry protein using Mouse Anti-mCherry Polyclonal Antibody (SPC-1268). Lane 1: 1:5,000 Dilution. Lane 2: 1:10,000 Dilution. Primary Antibody: Mouse Anti-mCherry Polyclonal Antibody (SPC-1268). Predicted/Observed Size: ~28kDa. Mouse Anti-mCherry Antibody used in Western blot (WB) on mCherry Recombinant Protein (SPC-1268)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Group
    Polyclonals and antibodies
  • About
    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
  • Gene target
    mCherry   ATTO   700  
  • Short name
    Mouse Anti-mCherry Polyclonal: ATTO 700
  • Technique
    Polyclonal, anti-, Mouse, anti, antibody to, antibodies, mouses, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Host
    mouse
  • Isotype
    IgG1
  • Label
    ATTO 700
  • Species
    Mouse, Mouses
  • Alternative name
    Mouse Antibody tomCherry polyclonal: ATTO 700
  • Alternative technique
    polyclonals, murine, antibodies
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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