Mouse Anti-Malondialdehyde (MDA) Monoclonal IgG1, Clone 6H6

  • Catalog number
    SMC-514D-A655
  • Price
    Please ask
  • Size
    100 µg
  • Clone
    6H6
  • Immunogen
    Synthetic Malondialdehyde modified Keyhole Limpet Hemocyanin (KLH).
  • Antibody s full description
    Mouse Anti- Malondialdehyde Monoclonal IgG1 Antibody, Clone: 6H6: ATTO 655
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    Malondialdehyde Antibody, MDA Antibody, Malondialdehyde (MDA) Antibody, Malonic aldehyde Antibody Propanedial Antibody, 1,3-Propanedial Antibody, Malonaldehyde Antibody
  • Raised in
    Mouse
  • Antibody s target
    Malondialdehyde
  • Primary research fields
    Cancer, Oxidative Stress, Lipid peroxidation, Neuroscience, Neurodegeneration, Alzheimer’s Disease
  • Brandname
    MOLECULAR SIGNATURE®
  • Antibodies applications
    WB, ICC/IF, FACS, FCM, ELISA
  • Antibody s reactivity
    Species Independent
  • Antibody s dilutions
    WB (1:1000); ICC/IF (1:50); FACS (1:50); FCM (1:50); ELISA (1:1000); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH 7.4, 50% glycerol, 0.9% Sodium Azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Specific for Malondialdehyde conjugated proteins. Does not detect free Malondialdehyde. Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Methylglyoxal modified proteins.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    A 1:1000 dilution of SMC-514 was sufficient for detection of Malondialdehyde in 2 µg of Malondialdehyde conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
  • Antibody in cell
    See included datasheet or contact our support service
  • Tissue specificity
    See included datasheet or contact our support service
  • Released date
    23-Mar-2017
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    Refer to NCBI
  • Gene number
    Refer to GenBank
  • Protein number
    Refer to Swiss-Prot
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 6H6 (SMC-514). Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody (SMC-514) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250: 1:1000 for 30-60 min at RT. (A,C,E,F) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain (C,D) Phalloidin Alex Fluor 633 F-Actin stain (E,F) Malondialdehyde Antibody (G,F) Composite. Courtesy of: Dr. Robert Burke, University of Victoria. Western Blot analysis of Malondialdehyde-BSA Conjugate showing detection of 67 kDa Malondialdehyde -BSA using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 6H6 (SMC-514). Lane 1: Molecular Weight Ladder (MW). Lane 2: Malondialdehyde-BSA (0.5 µg). Lane 3: Malondialdehyde-BSA (2.0 µg). Lane 4: BSA (0.5 µg). Lane 5: BSA (2.0 µg) . Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody (SMC-514) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa. Mouse Anti-Malondialdehyde Antibody [6H6] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Embryonic kidney cells (HEK293) (SMC-514) Mouse Anti-Malondialdehyde Antibody [6H6] used in Western Blot (WB) on Malondialdehyde-BSA Conjugate (SMC-514)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
  • Gene symbol
    SDCBP, IFIH1, IL24
  • Short name
    Mouse Anti-Malondialdehyde (MDA) Monoclonal IgG1, Clone 6H6
  • Technique
    anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
  • Host
    mouse
  • Isotype
    IgG1, IgG1
  • Label
    ATTO 655
  • Species
    Mouse, Mouses
  • Alternative name
    Mouse Antibody toMalondialdehyde (MDA) monoclonal IgG1, clonality 6H6
  • Alternative technique
    murine, antibodies
  • Clone name
    6H6
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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