Mouse Anti-Human ZAP-70-FITC

  • Catalog number
    GEN670108
  • Price
    Please ask
  • Size
    0.025 miligrams
  • Also known as
    ZAP-70 (TCR zeta)
  • Other names
    ZAP-70; Tyrosine-protein kinase ZAP-70; tyrosine-protein kinase ZAP-70; syk-related tyrosine kinase; 70 kDa zeta-associated protein; zeta-chain (TCR) associated protein kinase; 70 kDa zeta-associated protein; Syk-related tyrosine kinase
  • Category
    Antibodies
  • Subcategory
    Mnoclonal antibodies
  • Gene name
    Zap70; Zap70; Srk; TZK; mur; 70kDa; mrtle; ZAP-70; AI327364; Srk; Zap-70
  • Gene name synonims
    Zap70; Zap70; Srk; TZK; mur; 70kDa; mrtle; ZAP-70; AI327364; Srk; Zap-70
  • Other gene names
    Zap70; Zap70; Srk; TZK; mur; 70kDa; mrtle; ZAP-70; AI327364; Srk; Zap-70
  • Clonality
    Monoclonal
  • Immunoglobulin isotype
    Mouse IgG1 kappa
  • Clone
    SBZAP
  • Host organism
    Mouse (Mus musculus)
  • Species reactivity
    Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
  • Specificity and cross reactivity
    Human TCR zeta ; Zap-70 (Mr 70 kDa) ZAP 70, also known as TCR zeta associated protein, is a protein tyrosine kinase (PTK) with the primary role of catalyzing the phosphorylation of tyrosine residues in cellular proteins, and thus, contributes significantly to the initiation of cell signaling. ZAP 70 is expressed on T cells and NK cells. The monoclonal antibodies SB70 and SBZAP were generated against a KLH-peptide sequence corresponding to the human ZAP 70 amino acid residues 280-309.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
  • Purification method
    N/A
  • Form Appearance
    Fluorescein (FITC) Conjugate
  • Concentration
    N/A
  • Storage and shipping
    The purified antibody is supplied as 0.025 mg purified immunoglobulin in 0.25 mL or 0.1 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or aminecontaining buffer salts added. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The fluorescein (FITC) conjugate is supplied as 0.025 mg in 0.25 mL or 0.1 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store the antibody ats should be kept in the range of 1-7 degrees Celsius. or long-term at - 20 degree C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store the antibody ats should be kept in the range of 1-7 degrees Celsius. or long-term the antibody should be stored at -20 degrees Celsius.. The biotin (BIOT) conjugate is supplied as 0.025 mg in 0.25 mL or 0.1 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The R-phycoerythrin (R-PE) conjugate is supplied as 0.025 mg in 0.25 mL or 0.1 mg in 1.0 mL in PBS/NaN3 and a stabilizing agent. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. Do not freeze! Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
  • Tested applications
    ELISA; Flow Cytometry
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Test
    MBS Monoclonals supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Properties
    This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Conjugation
    Anti-FITC Antibody
  • Latin name
    Mus musculus
  • Gene target
    ZAP-70  
  • Gene symbol
    ZAP70, IGHV2-70, IGLVI-70, YLPM1, RNU6-70P, SWAP70, HLA-K, UCHL5, ZC3HAV1
  • Short name
    Mouse Anti- ZAP-70-FITC
  • Technique
    anti-Human, anti-, Mouse, anti, FITC, antibody to, antibodies, antibodies against human proteins, Fluorescein, mouses
  • Host
    mouse
  • Label
    FITC
  • Species
    Human, Humans, Mouses
  • Alternative name
    Anti-Mouse Anti-Human ZAP-70-FITC
  • Alternative technique
    murine, antibodies, fluorescine
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin lambda variable (I)-70 (pseudogene)
  • Synonyms gene name
    • immunoglobulin lambda variable (I)-70
    • immunoglobulin lambda variable (I)-70 pseudogene
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2000-05-08
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin lambda locus at 22q11.2
  • VEGA ID
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    RNA, U6 small nuclear 70, pseudogene
  • Synonyms gene
  • Synonyms gene name
    • RNA, U6 small nuclear 70
  • Locus
  • Discovery year
    2011-08-08
  • Entrez gene record
Gene info
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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