Mouse Anti-Human ZAP-70-FITC
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Catalog numberGEN670108
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PricePlease ask
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Size0.025 miligrams
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Also known asZAP-70 (TCR zeta)
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Other namesZAP-70; Tyrosine-protein kinase ZAP-70; tyrosine-protein kinase ZAP-70; syk-related tyrosine kinase; 70 kDa zeta-associated protein; zeta-chain (TCR) associated protein kinase; 70 kDa zeta-associated protein; Syk-related tyrosine kinase
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CategoryAntibodies
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SubcategoryMnoclonal antibodies
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Gene nameZap70; Zap70; Srk; TZK; mur; 70kDa; mrtle; ZAP-70; AI327364; Srk; Zap-70
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Gene name synonimsZap70; Zap70; Srk; TZK; mur; 70kDa; mrtle; ZAP-70; AI327364; Srk; Zap-70
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Other gene namesZap70; Zap70; Srk; TZK; mur; 70kDa; mrtle; ZAP-70; AI327364; Srk; Zap-70
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ClonalityMonoclonal
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Immunoglobulin isotypeMouse IgG1 kappa
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CloneSBZAP
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Host organismMouse (Mus musculus)
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Species reactivityHuman (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivityHuman TCR zeta ; Zap-70 (Mr 70 kDa) ZAP 70, also known as TCR zeta associated protein, is a protein tyrosine kinase (PTK) with the primary role of catalyzing the phosphorylation of tyrosine residues in cellular proteins, and thus, contributes significantly to the initiation of cell signaling. ZAP 70 is expressed on T cells and NK cells. The monoclonal antibodies SB70 and SBZAP were generated against a KLH-peptide sequence corresponding to the human ZAP 70 amino acid residues 280-309.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification methodN/A
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Form AppearanceFluorescein (FITC) Conjugate
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ConcentrationN/A
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Storage and shippingThe purified antibody is supplied as 0.025 mg purified immunoglobulin in 0.25 mL or 0.1 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or aminecontaining buffer salts added. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The fluorescein (FITC) conjugate is supplied as 0.025 mg in 0.25 mL or 0.1 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store the antibody ats should be kept in the range of 1-7 degrees Celsius. or long-term at - 20 degree C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store the antibody ats should be kept in the range of 1-7 degrees Celsius. or long-term the antibody should be stored at -20 degrees Celsius.. The biotin (BIOT) conjugate is supplied as 0.025 mg in 0.25 mL or 0.1 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The R-phycoerythrin (R-PE) conjugate is supplied as 0.025 mg in 0.25 mL or 0.1 mg in 1.0 mL in PBS/NaN3 and a stabilizing agent. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. Do not freeze! Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
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Tested applicationsELISA; Flow Cytometry
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestMBS Monoclonals supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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PropertiesThis MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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ConjugationAnti-FITC Antibody
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Latin nameMus musculus
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Gene target
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Gene symbolZAP70, IGHV2-70, IGLVI-70, YLPM1, RNU6-70P, SWAP70, HLA-K, UCHL5, ZC3HAV1
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Short nameMouse Anti- ZAP-70-FITC
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Techniqueanti-Human, anti-, Mouse, anti, FITC, antibody to, antibodies, antibodies against human proteins, Fluorescein, mouses
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Hostmouse
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LabelFITC
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SpeciesHuman, Humans, Mouses
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Alternative nameAnti-Mouse Anti-Human ZAP-70-FITC
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Alternative techniquemurine, antibodies, fluorescine
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Gene info
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Identity
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Gene
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Long gene namezeta chain of T cell receptor associated protein kinase 70
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Synonyms gene
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Synonyms gene name
- SRK
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1995-05-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- SH2 domain containing
- Syk family tyrosine kinases
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 2-70
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable (I)-70 (pseudogene)
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Synonyms gene name
- immunoglobulin lambda variable (I)-70
- immunoglobulin lambda variable (I)-70 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameYLP motif containing 1
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Synonyms gene
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Synonyms gene name
- chromosome 14 open reading frame 170
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-11-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Protein phosphatase 1 regulatory subunits
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 70, pseudogene
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Synonyms gene
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Synonyms gene name
- RNA, U6 small nuclear 70
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Locus
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Discovery year2011-08-08
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Entrez gene record
Gene info
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Identity
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Gene
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Long gene nameswitching B cell complex subunit SWAP70
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Synonyms gene name
- SWAP switching B-cell complex 70kDa subunit
- SWAP switching B-cell complex subunit 70
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2009-05-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- EF-hand domain containing
- Pleckstrin homology domain containing
- Minor histocompatibility antigens
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class I, K (pseudogene)
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Synonyms gene
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-11-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Histocompatibility complex
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameubiquitin C-terminal hydrolase L5
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Synonyms gene name
- ubiquitin carboxyl-terminal hydrolase L5
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Synonyms
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Synonyms name
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Locus
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Discovery year2002-11-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ubiquitin C-terminal hydrolases
- INO80 complex
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namezinc finger CCCH-type containing, antiviral 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-12-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Poly(ADP-ribose) polymerases
- Zinc fingers CCCH-type
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data