Mouse anti Human vitronectin receptor CD51

  • Catalog number
    MUB2011S
  • Price
    Please ask
  • Size
    1 mL
  • Category
    Primary Antibodies
  • Long description
    CD51 (Cluster of Differentiation 51), known as vitronectin receptor α chain, or integrin αV is a type I integral membrane glycoprotein. CD51 forms a heterodimer with integrin β1 (CD29), β3 (CD61), β5, β6, or β8. CD51 contains two disulfide-linked subunits of 125 kD and 24 kD. Many extracellular matrix proteins with RGD-motifs are CD51 ligands. In association with its β chains, CD51 binds vitronectin, von Willebrand factor, fibronectin, thrombospondin, osteopontin, fibrinogen, and laminin. CD51, as an adhesion molecule, plays important roles in leukocytes homing and rolling, mediates bone absorption and angiogenesis. CD51 is expressed on endothelial cells, fibroblasts, macrophages, platelets, osteoclasts, neuroblastoma, melanoma, and hepatoma cells.
  • Antibody come from
    NKI-M7 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with cultured melanoma cells derived from a Human melanoma metastasis.
  • Other description
    Each vial contains 1ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide.
  • Clone
    NKI-M7 (former AMF7)
  • Antigen antibody binding interaction
    Mouse anti Human vitronectin receptor CD51 Antibody
  • Antibody is raised in
    Mouse (Balb/c)
  • Antibody s reacts with
    Human
  • Antibody s reacts with these species
    This antibody doesn't cross react with other species
  • Antibody s specificity
    T-lymphocyte clones NKI-M7 detect a melanoma-associated proteoglycan (MW greater than 450-250 kDa) and a molecular complex, which under reducing conditions consists of 4 compounds of 120, 95, 29 and 25 kDa respectively. NKI-M7 reacts with cultured melanomas melanomas in frozen sections. The antigen detected by NKI-M7 is strongly associated with the adhesion and cytoplasmic spreading of melanoma cells to plastic surfaces and monolayers of vascular endothelial cells. NKI-M7 blocks fibronectin-induced chemotaxic motility and chemokinesis of melanoma cells. In addition to their membrane localization, the antigens detected by NKI-M7are abundant in extracellular adhesion plaques deposited by cultured melanoma cells. NKI-M7 stains primary tumors heterogeneously, whereas metastases are homogeneously stained.
  • Research interest
    CD markers,Cell adhesion
  • Application
    ELISA,Flow Cytometry,Immunohistochemistry (frozen),Immunoprecipitation
  • Antibody s suited for
    NKI-M7 is useful for ELISA, immunoprecipitation, flow cytometry and immunohistochemsitry on frozen sections. Optimal antibody dilution should be determined by titration.
  • Storage
    Store at 4°C, or in small aliquots at -20°C.
  • Relevant references
    1. Vries J.E. de, G.D. Keizer, A.A. te Velde, A. Voordouw, D. Ruiter, Ph. Rumke, H. Spits and C.G. Figdor. Int. J. Cancer 38: 465, 1986. 2. J.M.W. Quinn, N.A. Athanasou, and J.O'D McGee,1991. Histochemistry, 96:169-176
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
  • Gene symbol
    ITGAV
  • Short name
    Mouse anti vitronectin receptor CD51
  • Technique
    anti human, Mouse, anti, antibody to, anti human antibodies, mouses
  • Host
    mouse
  • Isotype
    IgG1
  • Label
    unlabeled
  • Species
    Human, Humans, Mouses
  • Alternative name
    Mouse antibody to H. sapiens vitronectin receptor CD51
  • Alternative technique
    murine, antibodies
Gene info
  • Identity
  • Gene
  • Long gene name
    integrin subunit alpha V
  • Synonyms gene
  • Synonyms gene name
    • antigen identified by monoclonal antibody L230
    • vitronectin receptor
    • integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)
    • integrin, alpha V
  • Synonyms
  • Locus
  • Discovery year
    1988-07-19
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • CD molecules
    • Integrin alpha subunits
  • VEGA ID
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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