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Clone
1A7
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Immunogen
Full-length protein.
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Antibody s full description
Mouse Anti-Human Vimentin Monoclonal IgG1 Antibody, Clone: 1A7: Unconjugated
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
CTRCT30 Antibody, Epididymis luminal protein 113 Antibody, HEL113 Antibody, VIM Antibody, VIME_HUMAN Antibody, Vimentin Antibody
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Raised in
Mouse
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Antibody s target
Vimentin
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Brandname
None
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Antibodies applications
WB, ICC/IF
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s dilutions
WB (1:2000), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
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Purity
Peptide Affinity Purified
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Antibody buffer for storage
PBS, pH 7.4, 50% glyercol, 0.05% sodium azide
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Antibody s specificity
Antibody can detects endogenous Vimentin protein.
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
A 1:2000 dilution of SMC-261 was sufficient for detection of Vimentin in 10 µg of Human, Mouse, Rat, HeLa, Brain cell lysates by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
Vimentin is a type III intermediate filament (IF) protein. All IF proteins are highly expressed and vimentin is the major cytoskeletal component of mesechymal, and therefore Vimentin is used as a marker of mesenchymally-derived cells or cells undergoing EMT during both normal development and metastic progression. Functionally it also serves a significant role in supporting and anchording the organelles in the cytosol. Clinical significance includes being used as a sarcoma tumor marker, and it has also been used in the diagnosis of rheumatoid arthritis.
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Bibliography
1. Katsumoto T., Mitsushima A., Kurimura T. (1990). Biol Cell. 68(2): 139–146. 2. Leader M, Collins M, Patel J, Henry K (1987). Histopathology 11(1): 63–72. 3. Immunohistochemistry from the Washington Animal Disease Diagnostic laboratory (WADDL)of the College of Veterinary Medicine, Washington State University. Retrieved 2009-03-14.
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Released date
06-Sep-2016
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
NP_003371.2
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Gene number
7431
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Protein number
P08670
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Antibody s datasheet
Contact our support service
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Vimentin Monoclonal Antibody, Clone 1A7 (SMC-261). Tissue: HeLa cell lysates. Species: Human. Primary Antibody: Mouse Anti-Vimentin Monoclonal Antibody (SMC-261) at 1:100. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: DAPI (blue) nuclear stain. Western Blot analysis of Human, Mouse, Rat HeLa, Brain cell lysates showing detection of ~50-57 kDa Vimentin protein using Mouse Anti-Vimentin Monoclonal Antibody, Clone 1A7 (SMC-261). Lane 1: Human HeLa. Lane 2: Mouse Brain. Lane 3: Rat Brain.. Primary Antibody: Mouse Anti-Vimentin Monoclonal Antibody (SMC-261) at 1:2000. Predicted/Observed Size: ~50-57 kDa. Mouse Anti-Vimentin Antibody [1A7] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa cell lysates (SMC-261) Mouse Anti-Vimentin Antibody [1A7] used in Western Blot (WB) on Human, Mouse, Rat HeLa, Brain cell lysates (SMC-261)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin name
Mus musculus
