Mouse Anti-Human VDAC1 Monoclonal IgG2a, Clone S152B-23
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Catalog numberSMC-456D
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PricePlease ask
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Size100 µg
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CloneS152B-23
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ImmunogenFusion protein amino acids 1-283 (full-length) of human VDAC1. Mouse: 98% identity (279/283 amino acids identical). Rat: 98% identity (279/283 amino acids identical) >60% identity with VDAC2 and VDAC3.
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Antibody s full descriptionMouse Anti-Human VDAC1 Monoclonal IgG2a Antibody, Clone: S152B-23: Unconjugated
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameVoltage Dependent Anion Channel 1 Antibody, Porin Antibody, Voltage dependent anion selective channel protein 1 Antibody, Voltage-dependent anion-selective channel protein 1 Antibody, hVDAC1 Antibody, MGC111064 Antibody, Mitochondrial Porin Antibody, Outer mitochondrial membrane protein porin 1 Antibody, Plasmalemmal porin Antibody, Porin 31HL Antibody, Porin 31HM Antibody, PORIN-31-HL Antibody, VDAC 1 Antibody, VDAC Antibody, VDAC-1 Antibody
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Raised inMouse
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Antibody s targetVDAC1
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Primary research fieldsNeuroscience, Ion Channels, Cell Signaling, Cancer
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH 7.4, 50% glycerol, 0.1% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~30kDa. Does not cross-react with VDAC2 or VDAC3 (based on KO validation results).
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-456 was sufficient for detection of VDAC1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellMitochondrion , Mitochondrion Outer Membrane , Cell Membrane
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextVoltage-dependent anion-selective channel protein 1 (also known as VDAC, VDAC1 or outer mitochondrial membrane protein porin 1) is the the outer mitochondrial membrane receptor for hexokinase and BCL2L1. VDAC forms a channel through the mitochondrial membrane and is involved in small molecule diffusion, cell volume regulation and apoptosis. VDAC may participate in the formation of the permeability transition pore complex (PTPC), which is responsible for the release of mitochondrial products that triggers apoptosis.
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Released date29-Oct-2013
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_003365.1.
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Gene number7416
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Protein numberP21796
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-VDAC1 Monoclonal Antibody, Clone S152B-23 (SMC-456). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VDAC1 Monoclonal Antibody (SMC-456) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Mitochondrion, Mitochondrion Outer Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) VDAC1 Antibody (D) Composite. Western Blot analysis of Rat Brain Membrane showing detection of ~30 kDa VDAC1 protein using Mouse Anti-VDAC1 Monoclonal Antibody, Clone S152B-23 (SMC-456). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-VDAC1 Monoclonal Antibody (SMC-456) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~30 kDa. Mouse Anti-VDAC1 Antibody [S152B-23] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-456) Mouse Anti-VDAC1 Antibody [S152B-23] used in Western Blot (WB) on Rat Brain Membrane (SMC-456)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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Gene target
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Gene symbolIGHV3-23, IGKV2D-23, SNORD116-23, VDAC1, SNORD114-23, SNORD115-23, IGKV2-23, ERVW-23, IGLV2-23, ERVK-23
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Short nameMouse Anti- VDAC1 Monoclonal IgG2a, Clone S152B-23
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Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG2a, IgG2a
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LabelUnconjugated
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SpeciesHuman, Humans, Mouses
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Alternative nameMouse Antibody toHuman voltage-dependent anion channel 1 monoclonal IgG2a, clonality S152B-23
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Alternative techniquemurine, antibodies
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Alternative to gene targetvoltage-dependent anion channel 1, PORIN and VDAC-1, VDAC1 and IDBG-240337 and ENSG00000213585 and 7416, porin activity, nuclei, Vdac1 and IDBG-171107 and ENSMUSG00000020402 and 22333, VDAC1 and IDBG-645740 and ENSBTAG00000013113 and 282119
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Clone nameS152B-23
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Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3-23
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 2D-23 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 2D-23
- immunoglobulin kappa variable 2D-23 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 116-23
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene namevoltage dependent anion channel 1
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Synonyms
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Locus
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Discovery year1993-11-02
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Entrez gene record
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Pubmed identfication
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Classification
- Voltage dependent anion channels
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 114-23
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Synonyms
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Locus
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Discovery year2006-09-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-23
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 2-23 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 2-23
- immunoglobulin kappa variable 2-23 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameendogenous retrovirus group W member 23
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Synonyms gene name
- endogenous retrovirus group W, member 23
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GenBank acession
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Locus
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Discovery year2013-10-21
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable 2-23
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameendogenous retrovirus group K member 23
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Synonyms gene name
- endogenous retrovirus group K, member 23
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Synonyms
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GenBank acession
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Locus
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Discovery year2011-05-05
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Pubmed identfication
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
