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Clone
FK2
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Immunogen
Ubiquitin conjugated lysozyme
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Antibody s full description
Mouse Anti-Human Ubiquitin Monoclonal IgG1 Antibody, Clone: FK2: Alkaline Phosphatase
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
Polyubiquitin B Antibody, RPS27A Antibody, UBA52 Antibody, UBB Antibody, UBC Antibody, ubiquitin B Antibody
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Raised in
Mouse
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Antibody s target
Ubiquitin
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Primary research fields
Cell Signaling, Post-translational Modifications, Ubiquitination
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Brandname
None
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Antibodies applications
WB, IHC, ICC/IF, IP, ELISA
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Antibody s reactivity
Species Independent
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Antibody s dilutions
WB (1:10000); optimal dilutions for assays should be determined by the user.
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Purity
Protein A Purified
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Antibody buffer for storage
10mM phosphate buffer, 0.15M NaCl pH7.4, 0.1% sodium azide
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Antibody s concentration
10 mg/ml
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Antibody s specificity
Detects ubiquitinated proteins and ubiquitin chains. Does not detect free ubiquitin.
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Storage recommendations
4ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
A 1:5000 dilution of SMC-214 was sufficient for detection of ubiquitin conjugates in 2.5 µg of HeLa cell lysate by electrochemiluminescence analysis using goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm , Nucleus
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
Ubiquitin is a small protein found ubiquitously in all tissue types and acts as a post translational modification. It can bind to its substrate either as a single ubiquitin molecule or in a chain. It is involved in many regulatory processes, which include proteasomal degradation, signal transduction, DNA repair, endocytosis and autophagy.
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Bibliography
1. Chen J., & Chen Z. (2013). Curr Opin Immunol. (1): 4-12. 2. Shaid S., Brandts C., Serve H., & Dikic I. (2013). Cell Death Differ. 20(1): 21-30.
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Released date
12-Jun-2014
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
BAC56955.1
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Gene number
7314
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Protein number
P0CG47
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Antibody s datasheet
Contact our support service
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone FK2 (SMC-214). Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody (SMC-214) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 60X. Mouse Anti-Ubiquitin Antibody [FK2] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Mouse Fibroblast cell line (NIH 3T3) (SMC-214)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin name
Mus musculus
