Mouse Anti-Human Trak2 Monoclonal IgG2A, Clone S390-43
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Catalog numberSMC-483D-ALP
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PricePlease ask
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Size100 µg
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CloneS390-43
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ImmunogenFusion protein amino acids 757-914 (C-terminus) of human TRAK2
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Antibody s full descriptionMouse Anti-Human TRAK2 Monoclonal IgG2A Antibody, Clone: S390-43: Alkaline Phosphatase
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameTrafficking kinesin-binding protein 2 Antibody, Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 3 protein Antibody, GABA-A receptor-interacting factor 1 Antibody, O-GlcNAc transferase-interacting protein of 98 kDa Antibody, EMBL AAH60681.1 Antibody, Ensembl ENSMUSP00000027186 MGI 1918077 Antibody, Als2cr3 Antibody, Grif1 Antibody, Oip98 Antibody, KIAA0549 Antibody
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Raised inMouse
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Antibody s targetTRAK2
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Primary research fieldsNeuroscience, Neurodegeneration, Alzheimer's Disease
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF
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Antibody s reactivityHuman, Rat
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Antibody s dilutionsWB (1:1000); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.1% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~100kDa. Does not cross-react with TRAK1.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysisA 1:100 dilution of SMC-483 was sufficient for detection of Trak2 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellCytoplasm , Mitochondrion , Early Endosome
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextTRAK2, a member of the TRAK family of adaptor proteins, links mitochondria to microtubule based motors. Localized in dendrites, it is specifically required for dendritic development as it reacts with dynein and dynactin. TRAK2 interferes with kinesin-1 binding and axonal transport (1). It has been shown to interact with Kir2.1 and GABA-R Beta2 (2, 3).
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Bibliography1. van Spronsen M. et al. (2013) Neuron. 77(3): 48-502. 2. Grishin A., Li H., Levitan E.S., Zaks E. (2006) J Biol Chem. 281(4): 30104-30111. 3. Beck M., et al. (2002) J Biol Chem. 277(33): 30079-30090.
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Released date10-Mar-2015
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_055864.2
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Gene number66008
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Protein numberO60296
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-Trak2 Monoclonal Antibody, Clone S390-43 (SMC-483). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Trak2 Monoclonal Antibody (SMC-483) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Trak2 Antibody (D) Composite. Western Blot analysis of Rat Brain-Membrane showing detection of ~ 100 kDa TRAK2 protein using Mouse Anti-TRAK2 Monoclonal Antibody, Clone S390-43 (SMC-483). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane. Load: 15 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-TRAK2 Monoclonal Antibody (SMC-483) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~ 100 kDa. Mouse Anti-Trak2 Antibody [S390-43] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-483) Mouse Anti-TRAK2 Antibody [S390-43] used in Western Blot (WB) on Rat Brain-Membrane (SMC-483)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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Gene target
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Gene symbolIGLV7-43, IGHVII-43-1, SNORD115-43, IGHV3-43, IGKV1D-43, RNU6-43P, SNORD17, TARDBP, CCZ1, RLIM
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Short nameMouse Anti- Trak2 Monoclonal IgG2A, Clone S390-43
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Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG2A, IgG2a
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LabelAlkaline Phosphatase
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SpeciesHuman, Humans, Mouses
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Alternative nameMouse Antibody toHuman Trak2 monoclonal IgG2A, clonality S390-43
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Alternative techniquemurine, antibodies
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Clone nameS390-43
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Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable 7-43
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (II)-43-1 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (II)-43-1
- immunoglobulin heavy variable (II)-43-1 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-43
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3-43
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1D-43
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 43, pseudogene
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Synonyms gene
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Synonyms gene name
- RNA, U6 small nuclear 43
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Locus
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Discovery year2008-06-10
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Entrez gene record
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 17
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Synonyms
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Locus
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Discovery year2006-05-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameTAR DNA binding protein
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-01-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- RNA binding motif containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameCCZ1 homolog, vacuolar protein trafficking and biogenesis associated
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Synonyms gene
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Synonyms gene name
- chromosome 7 open reading frame 28A
- CCZ1 vacuolar protein trafficking and biogenesis associated homolog (S. cerevisiae)
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-07-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namering finger protein, LIM domain interacting
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Synonyms gene
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Synonyms gene name
- ring finger protein 12
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2002-04-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ring finger proteins
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data