Mouse Anti-Human Syntaxin Monoclonal IgG1, Clone SP-6

  • Catalog number
    SMC-181C
  • Price
    Please ask
  • Size
    25 µg
  • Clone
    SP-6
  • Immunogen
    Raised against synaptic vesicle-containing fractions of immunoprecipitated human brain homogenate
  • Antibody s full description
    Mouse Anti-Human Syntaxin Monoclonal IgG1 Antibody, Clone: SP-6: Unconjugated
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    HPC1 Antibody, P35 1 Antibody, STX1 Antibody, STX1A Antibody, STX1B Antibody, STX2 Antibody, STX3 Antibody
  • Raised in
    Mouse
  • Antibody s target
    Syntaxin
  • Primary research fields
    Neuroscience, Cell Structure, Pre-Synaptic Markers, Cell Signaling, Trafficking, Cancer
  • Brandname
    None
  • Antibodies applications
    WB, IHC, ICC/IF
  • Antibody s reactivity
    Mouse, Rat
  • Antibody s dilutions
    WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH7.4, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~38kDa.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    1 µg/ml of SMC-181 was sufficient for detection of Syntaxin in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cytoplasmic Vesicle , Secretory Vesicle , Synaptic Vesicle Membrane , Cell Junction , Synapse , Cell Membrane
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    Syntax ins are a family of membrane integrated Q-SNARE proteins participating in exocytosis (1). They possess a single C-terminal transmembrane domain, a SNARE domain, and an N-terminal regulatory domain. This SNARE domain binds to synaptobrevin and SNAP-25 to form the SNARE complex (2, 3). Support of syntaxin’s involvement in vesicular transport and neurotransmission includes the requirement of its yeast homologs, SSO 1 and 2, in targeting and fusion of Golgi-derived vesicles with the plasma membrane (4). Syntaxin also provides the target pf clostridial neurotoxin C1, which blocks neurotransmitter release (5). The formation of 20S complex involves the interaction of VAMP with syntaxin through an ATP-dependent reaction modulated by synaptotagmin, SNAP-25, a-SNAP and NSF (6).
  • Bibliography
    1. Bennett M.K., et al. (1993) Cell. 74(5): 863-873. 2. Walter A.M., Wiederhold K., Bruns D., Fasshaurer D. and Sorensen J.B. (2010) J Cell BIol. 188(3): 401-413. 3. Honma S., Taki K., Lei S., and Wakisaka S. (2010) Anat Rec. Epub. 4. Aalto M.K., Ronne H. and Keranen S. (1993) EMBO J. 12: 4095-4104. 5. Blasi J., et al. (1993) EMBO J. 12: 4821-4828. 6. Sollner T., et al. (1993) Cell. 75: 409-418.
  • Released date
    30-Jun-2010
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    NP_004594.1
  • Gene number
    6804
  • Protein number
    Q16623
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
  • Representative figure legend
    Immunohistochemistry analysis using Mouse Anti-Syntaxin Monoclonal Antibody, Clone SP-6 (SMC-181). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Syntaxin Monoclonal Antibody (SMC-181) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Muscle positive, basal layer staining in the epidermis and positive staining in the hair follicles. Western Blot analysis of Rat tissue lysate showing detection of Syntaxin protein using Mouse Anti-Syntaxin Monoclonal Antibody, Clone SP-6 (SMC-181). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Syntaxin Monoclonal Antibody (SMC-181) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-Syntaxin Antibody [SP-6] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-181) Mouse Anti-Syntaxin Antibody [SP-6] used in Western Blot (WB) on Rat tissue lysate (SMC-181)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.025
  • Stock availability
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Test
    StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • Latin name
    Mus musculus
  • Gene target
  • Gene symbol
    SFTPA1, SP6, PHF7, REXO5, SPATA1, SPESP1, SFTA2, SFTPB, SFTPA2, FDCSP
  • Short name
    Mouse Anti- Syntaxin Monoclonal IgG1, Clone SP-6
  • Technique
    anti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
  • Host
    mouse
  • Isotype
    IgG1, IgG1
  • Label
    Unconjugated
  • Species
    Human, Humans, Mouses
  • Alternative name
    Mouse Antibody toHuman Syntaxin monoclonal IgG1, clonality SP-6
  • Alternative technique
    murine, antibodies
  • Clone name
    SP-6
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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