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Clone
JJ6
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Immunogen
Recombinant human full length p23 protein
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Antibody s full description
Mouse Anti-Human p23 Monoclonal IgG1 Antibody, Clone: JJ6: FITC
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
co chaperone p23 Antibody, PTGES3 Antibody, TEBP Antibody, telomerase binding protein p23 Antibody, unactive progesterone receptor 23kDa Antibody, HSP90 co-chaperone Antibody, Progesterone receptor complex p23 Antibody, Cytosolic prostaglandin E2 synthase Antibody
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Raised in
Mouse
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Antibody s target
p23
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Primary research fields
Cancer, Heat Shock
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Brandname
None
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Antibodies applications
WB, IHC, ICC/IF, IP, ELISA, AM
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Antibody s reactivity
Human, Mouse, Rabbit, Chicken, Guinea Pig (Cavia porcellus), Yeast, Yeast (Saccharomyces cerevisiae)
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Antibody s dilutions
WB (1:2000) IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
PBS, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~23kDa.
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
0.5 µg/ml of SMC-156 was sufficient for detection of p23 in 20 µg of heat shocked cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
p23 is a highly conserved ubiquitous protein, known to have an important function as a cochaperone for the HSP90 chaperoning system (1). Studies have revealed that p23 is a small protein (18 to 25 kDa) with a simple structure (2, 3). p23 does not have any structural homology with any other known proteins (1). p23 was first discovered as a part of the HSP90-progesterone receptor complex along with HSP70, p54 and p50 (1). p23 is a phosphor-protein, which is highly acidic and has an aspartic acid-rich c-terminal domain (1). Numerous studies have found p23 to be associated with other client proteins like Fes tyrosine kinase (4), the heme regulated kinase HRI (5), hsf1 transcription factor (4), aryl hydrocarbon receptor (4), telomerase (6), and Hepadnavirus reverse transcriptase (7). In spite of several years of study, the exact functional significance of p23 is still not clear (8). p23 is thought to be involved in the adenosine triphosphate–mediated HSP90 binding of client proteins (8). Since many HSP90 client proteins are involved in oncogenic survival signaling, a recent study has concluded p23 to be a promising target in leukemic apoptosis (9). HSP90 and its co-chaperone p23 are certainly among the emerging anti-tumor targets in oncology.
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Bibliography
1. Johnson J.L., Beito T. G., Krco C.J. & Toft D.O. (1994) Mol Cell Biol 14: 1956-63. 2. Weikl T., Abelmann K. & Buchner J. (1999) J Mol Biol 293: 685-91. 3. Weaver A.J., Sullivan W.P., Felts S.J., Owen B.A. & Toft, D.O. (2000) J Biol Chem 275: 23045-52. 4. Nair S.C., et al. (1996) Cell Stress Chaperones 1: 237-50. 5. Xu Z., et al. (1997) Eur J Biochem 246, 461-70. 6. Holt S.E., et al. (1999) Genes Dev 13: 817-26. 7. Hu J., Toft D., Anselmo D. & Wang X. (2002) J Virol 76: 269-79. 8. Felts, S.J. & Toft D.O. (2003) Cell Stress Chaperones 8: 108-13. 9. Gausdal G., Gjertsen B.T., Fladmark K.E., Demol H., Vandekerckhove J. & Doskeland S.O. (2004) Leukemia.
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Released date
1-Apr-2008
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PubMed number
22806877, 22806877
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Tested applications
Immunoprecipitation , Western Blot
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Tested reactivity
Human, Human
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NCBI number
NP_006592.3
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Gene number
10728
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Protein number
Q15185
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Antibody s datasheet
Contact our support service
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-p23 Monoclonal Antibody, Clone JJ6 (SMC-156). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-p23 Monoclonal Antibody (SMC-156) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-p23 Antibody. (C) Composite. Immunohistochemistry analysis using Mouse Anti-p23 Monoclonal Antibody, Clone JJ6 (SMC-156). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-p23 Monoclonal Antibody (SMC-156) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermal, dermal, HF, muscle staining. Bright dermal staining. Western Blot analysis of Human Cell lysates showing detection of p23 protein using Mouse Anti-p23 Monoclonal Antibody, Clone JJ6 (SMC-156). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-p23 Monoclonal Antibody (SMC-156) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-p23 Monoclonal Antibody, Clone JJ6 (SMC-156). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-p23 Monoclonal Antibody (SMC-156) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Uniform epidermal staining – some evidence of cell-cell borders. Mouse Anti-p23 Antibody [JJ6] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SMC-156) Mouse Anti-p23 Antibody [JJ6] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-156) Mouse Anti-p23 Antibody [JJ6] used in Western Blot (WB) on Human Cell lysates (SMC-156) Mouse Anti-p23 Antibody [JJ6] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SMC-156)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin name
Mus musculus
