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Clone
S138-6
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Immunogen
Fusion protein, amino acids 1-500 (N-terminus) of human LRRK2. 83% identical in mouse and rat. No significant identity with LRRK1.
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Antibody s full description
Mouse Anti-Human LRRK2 Monoclonal IgG1 Antibody, Clone: S138-6: Biotin
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
Leucine-rich repeat kinase 2 Antibody, RIP7 Antibody, PARK8 Antibody, Dardarin Antibody, ROCO 2 Antibody, RIPK7 Antibody, Leucine-rich repeat serine/threonine-protein kinase 2 Antibody, augmented in rheumatoid arthritis 17 Antibody
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Raised in
Mouse
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Antibody s target
LRRK2
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Primary research fields
Neuroscience, Neurodegeneration, Parkinson's Disease, Cell Signaling, Post-translationalmodification, Phosphorylation
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Brandname
None
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Antibodies applications
WB, ICC/IF
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s dilutions
WB (1:1000); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
PBS pH 7.4, 50% glycerol, 0.1% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects >200kDa.
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
1 µg/ml of SMC-446 was sufficient for detection of LRRK2/Dardarin in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm , Membrane , Mitochondrion , Golgi Apparatus , Cell Projection , Axon , Dendrite , Endoplasmic Reticulum , Cytoplasmic Vesicle , Endosome , Lysosome
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
LRRK2 is a large protein with multiple domains including several ankyrin, leucine-rich, and WD40 repeats, a Ras-like small GTPase family domain named Roc, and a kinase domain that is closely related to the RIP kinase domain. LRRK2 gene is expressed in brain as well as in other tissues such as lung, liver and heart. LRRK2 might be central to the pathogenesis of several major neurodegenerative diseases associated with parkinsonism. Several dominantly inherited missense mutations in the gene encoding LRRK2 have been identified in several families that exhibit a broad spectrum of neuropathological features.
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Released date
29-Oct-2013
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
NP_940980
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Gene number
120892
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Protein number
Q5S007
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Antibody s datasheet
Contact our support service
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-LRRK2/Dardarin Monoclonal Antibody, Clone S138-6 (SMC-446). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-LRRK2/Dardarin Monoclonal Antibody (SMC-446) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) LRRK2/Dardarin Antibody (D) Composite. Western Blot analysis of Rat Brain Membrane showing detection of >200 kDa LRRK2/Dardarin protein using Mouse Anti-LRRK2/Dardarin Monoclonal Antibody, Clone S138-6 (SMC-446). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-LRRK2/Dardarin Monoclonal Antibody (SMC-446) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: >200 kDa. Mouse Anti-LRRK2/Dardarin Antibody [S138-6] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-446) Mouse Anti-LRRK2/Dardarin Antibody [S138-6] used in Western Blot (WB) on Rat Brain Membrane (SMC-446)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin name
Mus musculus
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Gene target
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Gene symbol
LRRK2-DT, LRRK2, MIR1302-6, PIRC24, PIRC28, PIRC26, ADCP1, PIRC27
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Short name
Mouse Anti- LRRK2/Dardarin Monoclonal IgG1, Clone S138-6
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Technique
anti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
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Host
mouse
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Isotype
IgG1, IgG1
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Label
Biotin
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Species
Human, Humans, Mouses
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Alternative name
Mouse Antibody toHuman leucine-rich repeat kinase 2/Dardarin monoclonal IgG1, clonality S138-6
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Alternative technique
murine, antibodies
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Alternative to gene target
leucine-rich repeat kinase 2, LRRK2 and IDBG-27257 and ENSG00000188906 and 120892, transferase activity, Extracellular, Lrrk2 and IDBG-175625 and ENSMUSG00000036273 and 66725, LRRK2 and IDBG-633456 and ENSBTAG00000016260 and 535652
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Clone name
S138-6
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