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Clone
VB2
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Immunogen
Peptide derived from C-terminal region of human LC3C protein
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Antibody s full description
Mouse Anti-Human LC3C Monoclonal IgG1 Antibody, Clone: VB2: ATTO 655
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
MAP1LC3C Antibody, ATG8J Antibody, Autophagy-related protein LC3 C Antibody, Autophagy-related ubiquitin-like modifier LC3 C Antibody, LC3 like protein 2 Antibody, MAP1 light chain 3 like protein 2 Antibody, MAP1 light chain 3 like protein 3 Antibody, MAP1 light chain 3-like protein 3 Antibody, MAP1A/MAP1B LC3 C Antibody, MAP1A/MAP1B light chain 3 C Antibody, MAP1LC3C Antibody, Microtubule-associated protein 1 light chain 3 gamma Antibody, Microtubule-associated proteins 1A/1B light chain 3C Antibody, MLP3C_HUMAN Antibody
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Raised in
Mouse
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Antibody s target
LC3C
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Primary research fields
Cancer, Autophagy
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Brandname
None
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Antibodies applications
WB, IP
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s dilutions
WB (1:10000); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
10 mM sodium phosphate, pH7.2, 150 mM NaCl, 0.02% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Specific for LC3C. Does not detect LC3A, LC3B, GABARAP, GABARAPL1 or GABARAPL2.
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Storage recommendations
4ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
A 1:20000 dilution of SMC-212 was sufficient for detection of LC3C in lysate from cells expressing recombinant GST-tagged LC3C by electrochemiluminescence analysis using goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm , Cytoskeleton , Cytoplasmic Vesicle , Autophagosome , Autophagosome Membrane
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
Light chain 3 (LC3) is a microtubule-associated protein with an approximate molecular mass of 17kDa, and can be found ubiquitously throughout mammalian tissue. LC3 plays a role in autophagy; once the autophagic process is initiated in a cell, LC3 is conjugated to phosphatidylethanolamine to form LC3-II. This molecule is recruited to the autophagosome at the time of fusion with lysosomes, and LC3-II in autolysosomal lumen is degraded. Therefore, monitoring LC3 is an important tool for detecting autophagy and autophagy-related processes. LC3C is one of three isoforms which exhibits the lowest expression relative to the other two isoforms. Its highest expression is seen in placenta, lung and ovary.
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Bibliography
1. Tanida, I., Ueno, T., & Kominami, E. (2008). LC3 and Autophagy. Methods Mol Biol. 445: 77-88. 2. He, H., Dang, Y., Dai, F., Guo, Z., Wu, J., She, X., et al. (2003). J Biol Chem. 278(31): 29278-87.
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Released date
12-Jun-2014
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
NP_001004343.1
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Gene number
440738
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Protein number
Q9BXW4
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Antibody s datasheet
Contact our support service
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.05
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin name
Mus musculus
