Mouse anti Human HLA Class I Heavy Chain (Restricted expression)

  • Catalog number
    MUB2037P
  • Price
    Please ask
  • Size
    0,1mg
  • Category
    Primary Antibodies
  • Long description
    The HLA class I gene family is composed of a group of genes whose products encode cell surface glycoproteins of MW 40–45 kDa, associated non-covalently with the beta-2-microglobulin light chain. They include the three polymorphic molecules HLA-A, -B, and -C, which are ubiquitously expressed and which are able to present intracellular peptides to cytotoxic T cells. Three additional class I genes are known, commonly referred to as non-classical or class Ib genes, all highly homologous to the other class I genes and all of which associate with beta-2-microglobulin light chain. In humans, each of the class Ib genes appears to exhibit a distinct pattern of expression in developing and adult tissues. HLA-E transcripts are distributed widely in adult tissues and have also been found in the placenta and fetal liver. In the adult, the presence of HLA-F has been shown in skin, resting T cells, and B cells, whereas its expression during development has been reported in fetal liver and at low levels in placenta and extra-placental tissues. HLA-G was originally thought to be expressed only in certain populations of placental trophoblasts, but low levels have also been found in a variety of human tissues. Recently it was shown that HLA class I expression in breast cancer cells can have a predictive value for chemotherapy response.
  • Antibody come from
    HC10 is a mouse monoclonal IgG2a antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from BALB/c mice immunized with HLA-B7 and -B40 heavy chains.
  • Other description
    Each vial contains 100 µl 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
  • Clone
    HC10
  • Antigen antibody binding interaction
    Mouse anti Human HLA Class I Heavy Chain (Restricted expression) Antibody
  • Antibody is raised in
    Mouse
  • Antibody s reacts with
    Human
  • Antibody s reacts with these species
    This antibody doesn't cross react with other species
  • Antibody s specificity
    The mouse monoclonal antibody HC10 recognizes HLA class I heavy chains. HC10 reacts mostly with HLA-B and HLA-C heavy chains and some HLA-A (HLA-A10, HLA-A28, HLA-A29, HLA-A30, HLA-A31, HLA-A32, HLA-A33).
  • Research interest
    Immunology,Inflammation
  • Application
    Enzyme Linked Immuno Sorbent Assay, Flow cytometry, Immunohistochemistry on frozen and paraffin sections, Immunocytochemistry, Immunoprecipitation, Western blotting, Immuno-cryo-electron microscopy.
  • Antibody s suited for
    HC10 was raised against free class I heavy chains of HLA antigens to obtain antibodies that would still react with denatured class I antigens, as they occur in Western blotting, conventional light microscopical analysis of formalin-fixed, paraffin-embedded sections, and cryo-immuno-electron microscopy. HC10 indeed retains strong reactivity with free class I heavy chains in Western blots. HC10 also produces strong reactivity in immuno-electron microscopy. Its use allows the determination of tissue and subcellular distribution of class I antigens. Optimal antibody dilutions for the different applications should be determined by titration; recommended range is 1:100 – 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:1000 for immunoblotting applications.
  • Storage
    Store at 4°C, or in small aliquots at -20°C.
  • Relevant references
    1) Stam NJ, Vroom TM, Peters PJ, Pastoors EB, Ploegh HL. HLA-A- and HLA-B-specific monoclonal antibodies reactive with free heavy chains in western blots, in formalin-fixed, paraffin-embedded tissue sections and in cryo-immuno-electron microscopy. Int. Immunol. 1990;2:113-125. 2) de Kruijf EM, van Nes JGH, Sajet A, Tummers QRJG, Putter H, Osanto S, Speetjens FM, Smit VTHBM, Liefers GJ, van de Velde CJH, Kuppen PJK. The predictive value of HLA Class I tumor cell expression and presence of intratumoral Tregs for chemotherapy in patients with early breast cancer. Clin Cancer Res 2010;16:1272-1280. 3) Gauster M, Blashitz A, Dohr G. Monoclonal antibody HC10 does not bind to HLA-G. Rheumatology 2007; 46: 892-893. 4) Perosa F, Luccarelli G, Prete M, Favoino E, Ferrone S, Dammacco F. Beta-2-Microglobulin-free HLA Class I heavy chain epitope mimicry by monoclonal antibody HC-10-specific peptide. J Immunol 2003;171:1918-1926. 5) Hutter H, Hammer A, Blaschitz A, Hartmann M, Ebbesen P, Dohr G, Ziegler A, Uchanska-Ziegler B. Expression of HLA class I molecules in human first trimester and term placenta trophoblast. Cell Tissue Res 1996; 286:439–447.
  • Protein number
    see ncbi
  • Warnings
    no dangerous / no information available then contact us to know
  • Antibody s link
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
    HLA   Class   Heavy   Chain   Restricted   expression  
  • Gene symbol
    HLA-DPA2, HLA-DOA, HLA-DQA1, HLA-N, HLA-DPB1, HLA-DQA2, HLA-DQB2, HLA-W, HLA-DRA, HLA-S
  • Short name
    Mouse anti HLA Class I Heavy Chain (Restricted expression)
  • Technique
    anti human, Mouse, anti, antibody to, anti human antibodies, mouses
  • Host
    mouse
  • Isotype
    IgG2a
  • Label
    unlabeled
  • Species
    Human, Humans, Mouses
  • Alternative name
    Mouse antibody to H. sapiens Human leukocyte antigen Class I Heavy epitope (Restricted expression)
  • Alternative technique
    murine, antibodies
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
MeSH Data
  • Name
    Blotting, Western
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products
Mouse anti HLA Class I
Catalog number: MUB2035P
Size: 0,5mL
Price: Please ask
Mouse anti Human HLA Class I Heavy Chain (Restricted expression)
Catalog number: MUB2036P
Size: 0,1mg
Price: Please ask
Mouse Monoclonal [clone A4] (IgG1) to Human HLA Class I Free Chain Antibody
Catalog number: GEN245797
Size: 50ug
Price: Please ask
Mouse Anti-Human HLA Class I Heavy Chain (Restricted expression) Antibody
Catalog number: GEN570333
Size: 100ug
Price: Please ask
Mouse Anti-Human HLA Class I Heavy Chain (Restricted expression) Antibody
Catalog number: GEN570339
Size: 100ug
Price: Please ask
Mouse Anti-Human HLA Class I Heavy Chain (Restricted expression)
Catalog number: GEN570333
Size: 100ug
Price: Please ask
Mouse Anti-Human HLA Class I Heavy Chain (Restricted expression)
Catalog number: GEN570339
Size: 100ug
Price: Please ask
Mouse anti HLA Class I
Catalog number: MBS570324
Size: 0.5 mL
Price: Please ask
Mouse anti Human HLA Class I Heavy Chain (Restricted expression)
Catalog number: MBS570333
Size: 0.1 mg
Price: Please ask
Mouse anti Human HLA Class I Heavy Chain (Restricted expression)
Catalog number: MBS570339
Size: 0.1 mg
Price: Please ask
Mouse anti HLA Class I
Catalog number: MBS570324
Size: 0,5 mL
Price: Please ask
Mouse anti Human HLA Class I Heavy Chain (Restricted expression)
Catalog number: MBS570333
Size: 0,1 mg
Price: Please ask
Mouse anti Human HLA Class I Heavy Chain (Restricted expression)
Catalog number: MBS570339
Size: 0,1 mg
Price: Please ask
Mouse anti HLA Class I
Catalog number: MBS570324
Size: 5x0.5 mL
Price: Please ask
Mouse anti Human HLA Class I Heavy Chain (Restricted expression)
Catalog number: MBS570333
Size: 5x0.1 mg
Price: Please ask
Mouse anti Human HLA Class I Heavy Chain (Restricted expression)
Catalog number: MBS570339
Size: 5x0.1 mg
Price: Please ask
Filters
Mouse anti HLA Class I Heavy Chain Restricted expression)
Contact
Back
Chat with gentaur.com employee