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Clone
EP190B
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Immunogen
GST-human EPAS-1 (HIF2alpha) amino acids 535-631 fusion protein
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Antibody s full description
Mouse Anti-Human HIF 2 alpha Monoclonal IgG1 Antibody, Clone: EP190B: Streptavidin
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
ECYT4 Antibody, EPAS1 Antibody, HIF2alpha Antibody, HIF1 Alpha like factor Antibody, HLF Antibody, Hypoxia inducible factor 2 alpha Antibody, MOP2 Antibody, PASD2 Antibody
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Raised in
Mouse
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Antibody s target
HIF 2 alpha
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Primary research fields
Cancer, Oxidative Stress, Cell Signaling, Epigenetics
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Brandname
None
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Antibodies applications
WB, IHC, ICC/IF, ELISA, AM
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Antibody s reactivity
Human, Mouse
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Antibody s dilutions
WB (1:500), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~100kDa. Specific for HIF2 Alpha.
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
1 µg/ml of SMC-185 was sufficient for detection of HIF2α in 20 µg of CoCl2-induced Hela cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cell
Nucleus
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
Members of the hypoxia-inducible factor (HIF) family of transcription factors regulate the cellular response to hypoxia (1). HIF2-Alpha is involved in catecholamine homeostasis, vascular remodeling, physiological angiogenesis and adipogenesis. It is overexpressed in many cancerous tissues, but its exact role in tumourprogression remains to be clarified (2). Studies suggest that in the case of non-small cell lung cancer, HIF2-Alpha actually is a promoter of tumor growth and progression in a solid tumor (1). Other data suggests that HIF2-Alpha is an important regulator of innate immunity, and therefore may be useful in the therapeutic target for treating inflammatory disorders and cancer (3).
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Bibliography
1. Kim W.Y., et al. (2009) J Clin Invest. 119(8): 2160-2170. 2. Favier J., Lapointe S., Maliba R. and Sirois M.G. (2007) BMC Cancer. 7:139. 3. Imtiyaz H.Z., et al. (2010) J Clin Invest. 120(8): 2699-2714.
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Released date
10-Sep-2010
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PubMed number
21947252
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Tested applications
Western Blot
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Tested reactivity
Human
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NCBI number
NP_001421.4
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Gene number
2034
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Protein number
Q99814
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Antibody s datasheet
Contact our support service
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HIF2 alpha Monoclonal Antibody, Clone EP190b (SMC-185). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-HIF2 alpha Monoclonal Antibody (SMC-185) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Mouse (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Localizes to the nucleus upon hypoxia. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-HIF2 alpha Antibody. (C) Composite. Western Blot analysis of Human HeLa cell lysates showing detection of HIF2 alpha protein using Mouse Anti-HIF2 alpha Monoclonal Antibody, Clone EP190b (SMC-185). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HIF2 alpha Monoclonal Antibody (SMC-185) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Cells CoCl treated. Immunohistochemistry analysis using Mouse Anti-HIF2 alpha Monoclonal Antibody, Clone EP190b (SMC-185). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HIF2 alpha Monoclonal Antibody (SMC-185) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Basal cell staining in the epidermis, dermis positive and muscle positive. . Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HIF2 alpha Monoclonal Antibody, Clone EP190b (SMC-185). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-HIF2 alpha Monoclonal Antibody (SMC-185) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Localizes to the nucleus upon hypoxia. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-HIF2 alpha Antibody. (C) Composite. Mouse Anti-HIF2 alpha Antibody [EP190b] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SMC-185) Mouse Anti-HIF2 alpha Antibody [EP190b] used in Western Blot (WB) on Human HeLa cell lysates (SMC-185) Mouse Anti-HIF2 alpha Antibody [EP190b] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-185) Mouse Anti-HIF2 alpha Antibody [EP190b] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SMC-185)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
The Mouse Anti- HIF2 alpha Monoclonal IgG1, Clone EP190B is a α- or alpha protein sometimes glycoprotein present in blood. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin name
Mus musculus