-
Clone
6H4-2G7
-
Immunogen
His-tagged human GRP78
-
Antibody s full description
Mouse Anti-Human GRP78 Monoclonal IgG1 Antibody, Clone: 6H4-2G7: Alkaline Phosphatase
-
Antibody s category
Monoclonal Antibodies
-
Antibody s other name
78 kDa glucose regulated protein Antibody, 78 kDa glucose-regulated protein Antibody, AL022860 Antibody, AU019543 Antibody, BIP Antibody, D2Wsu141e Antibody, D2Wsu17e Antibody, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 Antibody, Endoplasmic reticulum lumenal Antibody, Ca2+ binding protein grp78 Antibody, FLJ26106 Antibody, Glucose Regulated Protein 78kDa Antibody, GRP 78 Antibody, GRP-78 Antibody, GRP78_HUMAN Antibody, Heat shock 70 kDa protein 5 Antibody, Heat Shock 70kDa Protein 5 Antibody, HSCe70 Antibody, HSPA 5 Antibody, HSPA5 Antibody, Immunoglobulin Heavy Chain Binding Protein Antibody, Immunoglobulin heavy chain-binding protein Antibody, mBiP Antibody, MIF2 Antibody, Sez7 Antibody
-
Raised in
Mouse
-
Antibody s target
GRP78
-
Primary research fields
Cancer, Heat Shock, Cell Signaling, Trafficking, Chaperones, Organelle Markers
-
Brandname
None
-
Antibodies applications
WB, ICC/IF, ELISA
-
Antibody s reactivity
Human, Mouse, Rat, Bovine, Fungi, Chestnut Blight (Cryphonectria parasitica), Hamster, Monkey, Rabbit, African clawed frog (Xenopus laevis)
-
Antibody s dilutions
WB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
-
Purity
Protein G Purified
-
Antibody buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
-
Antibody s concentration
1 mg/ml
-
Antibody s specificity
Detects ~78kDa.
-
Storage recommendations
-20ºC
-
Shipping recommendations
Blue Ice or 4ºC
-
Antibody certificate of analysis
0.5 µg/ml of SMC-196 was sufficient for detection of Grp78 in 10 µg of HeLa cell lysate by ECL immunoblot analysis.
-
Antibody in cell
Endoplasmic Reticulum , Endoplasmic Reticulum Lumen , Melanosome
-
Tissue specificity
See included datasheet or contact our support service
-
Scientific context
GRP78 is a ubiquitously expressed, 78-kDa glucose- regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' non-translated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Lou et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer’s patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs (6, 7).
-
Bibliography
1. Cho S., et al. (2007) Mol Cell Biol. 27(1): 368-83. 2. Yang Y., et al. (1998) J Biol Chem 273: 25552-25555. 3. Luo S., et al (2006) 26 (15): 5688-97. 4. Yu Z., et al. (1999) Exp Neurol. 15: 302-314. 5. Koomagi R., et al. (1999) Anticancer Res. 19:4333-4336. 6. Laquerre S., et al. (1998) J. Virology 72: 4940-4949. 7. Dong D., et al. (2005) Cancer Res 65(13): 5785-91.
-
Released date
1-Aug-2011
-
Tested applications
To be tested
-
Tested reactivity
To be tested
-
NCBI number
NP_001156906.1
-
Gene number
14828
-
Protein number
P20029
-
Antibody s datasheet
Contact our support service
-
Representative figure link
-
Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GRP78 Monoclonal Antibody, Clone 6H4-2G7 (SMC-196). Tissue: Cervical Cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody (SMC-196) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Endoplasmic Reticulum, Endoplasmic Reticulum Lumen. Magnification: 60X. Western Blot analysis of Human recombinant cell lysate showing detection of GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 6H4.2G7 (SMC-196). Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody (SMC-196) at 1:1000. Mouse Anti-GRP78 Antibody [6H4-2G7] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Cervical Cancer cell line (HeLa) (SMC-196) Mouse Anti-GRP78 Antibody [6H4.2G7] used in Western Blot (WB) on Human recombinant cell lysate (SMC-196)
-
Warning information
Non-hazardous
-
Country of production
Canada
-
Total weight kg
1.4
-
Net weight g
0.1
-
Stock availability
In Stock
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Test
StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
-
Latin name
Mus musculus
