Mouse Anti-Human FKBP51 Monoclonal IgG, Clone Hi51B

  • Catalog number
    SMC-138C
  • Price
    Please ask
  • Size
    25 µg
  • Clone
    Hi51B
  • Immunogen
    Synthetic peptide corresponding to the residues of human FKBP51
  • Antibody s full description
    Mouse Anti-Human FKBP51 Monoclonal IgG Antibody, Clone: Hi51B: Unconjugated
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    AIG6 Antibody, FK506 binding protein 5 Antibody, FKBP5 Antibody, FKBP54 Antibody, HSP90 binding immunophilin Antibody, p54 Antibody, Pplase Antibody, Ptg10 Antibody, Rotamase Antibody, T cekk FK506 binding protein Antibody
  • Raised in
    Mouse
  • Antibody s target
    FKBP51
  • Primary research fields
    Cancer, Heat Shock, Cell Signaling, Trafficking
  • Brandname
    None
  • Antibodies applications
    WB, ICC/IF
  • Antibody s reactivity
    Human, Mouse, Rat, Rabbit, Dog, Hamster
  • Antibody s dilutions
    WB (1:2000), ICC/IF (1:1000); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~51kDa.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    A 1:2000 dilution was sufficient for detection of FKBP51 in ~50 µg total protein using WB analysis.
  • Antibody in cell
    Cytoplasm , Nucleus
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    HSP90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones (1). One class of HSP90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an HSP90 binding site. Among the TPR cochaperones of HSP90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin A-binding families of immunophilins (2). FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex (3). The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the HSP90 (4). Although FKBP52 and FKBP51 share ~75% sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different HSP90-binding characteristics (3). FK506 binding protein 51 kDa (FKBP51 or otherwise referred to as FKBP54) has been identified as a progestininducible gene. This protein is predominantly expressed in murine T cells but in humans, it is abundantly expressed in numerous tissues at levels many times higher than FKBP12. The FKBP51 gene is known to be induced by glucocorticoids (5).
  • Bibliography
    1. Cheung-Flynn J., Roberts P.J., Riggs D.L., and Smith D.F.(2003) J. Biol. Chem. 278(19): 17388-17394. 2. Davies T.H., Ning Y.N., and Sanchez E.R. (2002) J Biol. Chem. 277 (7): 4597-4600. 3. Wu B. et al. (2004) Proc. Natl. Acad. Sci. USA. 101(22): 8348-8353. 4. Denny W.B., Prapapanich V., Smith D.F., and Scammell J.G. (2005) Endocrinology 146(7): 3194-3201. 5. Hubler T.R. et al. (2003) Endocrinology 144(6): 2380- 2387.
  • Released date
    1-Nov-2008
  • PubMed number
    26242202
  • Tested applications
    Western Blot
  • Tested reactivity
    Human
  • NCBI number
    NP_001139247.1
  • Gene number
    2289
  • Protein number
    Q13451
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-FKBP51 Monoclonal Antibody, Clone Hi51B (SMC-138). Tissue: MK cells. Species: Mouse. Primary Antibody: Mouse Anti-FKBP51 Monoclonal Antibody (SMC-138) at 1:1000. Secondary Antibody: APC Goat Anti-Mouse (red). Counterstain: DAPI (blue) nuclear stain. Courtesy of: the Hospital Henri Mondor, France. Western Blot analysis of Human HeLa cell lysates showing detection of FKBP51 protein using Mouse Anti-FKBP51 Monoclonal Antibody, Clone Hi51B (SMC-138). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-FKBP51 Monoclonal Antibody (SMC-138) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-FKBP51 Antibody [Hi51B] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Mouse MK cells (SMC-138) Mouse Anti-FKBP51 Antibody [Hi51B] used in Western Blot (WB) on Human HeLa cell lysates (SMC-138)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.025
  • Stock availability
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Test
    StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • Latin name
    Mus musculus
  • Gene target
  • Gene symbol
    FKBP5
  • Short name
    Mouse Anti- FKBP51 Monoclonal IgG, Clone Hi51B
  • Technique
    anti-Human, anti-, Mouse, anti, IgG, antibody to, antibodies, antibodies against human proteins, IgGs, Monoclonals or monoclonal antibodies, mouses
  • Host
    mouse
  • Isotype
    IgG, IgG
  • Label
    Unconjugated
  • Species
    Human, Humans, Mouses
  • Alternative name
    Mouse Antibody toHuman FKBP51 monoclonal Immunoglobulin G, clonality Hi51B
  • Alternative technique
    murine, antibodies, immunoglobulins
  • Clone name
    Hi51B
Gene info
  • Identity
  • Gene
  • Long gene name
    FKBP prolyl isomerase 5
  • Synonyms gene name
    • FK506-binding protein 5
    • FK506 binding protein 5
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1997-06-09
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Tetratricopeptide repeat domain containing
    • FKBP prolyl isomerases
    • MicroRNA protein coding host genes
  • VEGA ID
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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