Mouse Anti-Human EAAC1 Monoclonal IgG1, Clone S180-41
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Catalog numberSMC-406D-A594
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PricePlease ask
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Size100 µg
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CloneS180-41
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ImmunogenFusion protein amino acids 1-524 (full length) of human EAAC1
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Antibody s full descriptionMouse Anti-Human EAAT3 Monoclonal IgG1 Antibody, Clone: S180-41: ATTO 594
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameExcitatory amino-acid carrier 1 Antibody, excitatory amino acid transporter 3 Antibody, EAAT3 Antibody, Neuronal and epithelial glutamate transporter Antibody, Sodium-dependent glutamate/aspartate transporter 3 Antibody, solute carrier family 1 member 1 and Slc1a1 Antibody
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Raised inMouse
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Antibody s targetEAAT3
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Primary research fieldsNeuroscience, Cell Structure, Membrane Markers, Pumps/Transporters, Neurotransmitter Receptors
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BrandnameNone
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Antibodies applicationsWB, IP, ICC/IF
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Antibody s reactivityHuman, Rat
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Antibody s dilutionsWB (1:1000), ICC/IF (1:100);optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~60kDa.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis5 µg of SMC-406 was sufficient for immunoprecipitation of EAAC1 from 50 µg of rat brain lysate.
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Antibody in cellMembrane
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextSLC1A1/EAAT3/EAAC1, a member of this transporter family, is expressed throughout the body, particularly in brain, intestine, liver, heart and kidney. Functional defects of the SLC1A1 gene are found in patients of dicarboxylicamino aciduria.
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Bibliography1. Smith C. P., et al., (1994) Genomics 20:335-336. 2. Kanai Y., and Hediger M. A. (1992) Nature 360:467-471.
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Released date1-Apr-2012
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_004161.4
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Gene number6505
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Protein numberP43005
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-EAAC1 Monoclonal Antibody, Clone S180-41 (SMC-406). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-EAAC1 Monoclonal Antibody (SMC-406) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Membrane . Magnification: 60X. Western Blot analysis of Rat brain membrane lysate showing detection of EAAT3 protein using Mouse Anti-EAAT3 Monoclonal Antibody, Clone S180-41 (SMC-406). Primary Antibody: Mouse Anti-EAAT3 Monoclonal Antibody (SMC-406) at 1:1000. Mouse Anti-EAAC1 Antibody [S180-41] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human SK-N-BE Cells (Human Neuroblastoma cells) (SMC-406) Mouse Anti-EAAT3 Antibody [S180-41] used in Western Blot (WB) on Rat brain membrane lysate (SMC-406)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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Gene target
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Gene symbolSNORD115-41, SLC1A1, IGHV3-41, IGLVVII-41-1, IGLV1-41, IGKV6D-41, CCDC18, TRIM71, RNU6-41P, METTL25B
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Short nameMouse Anti- EAAC1 Monoclonal IgG1, Clone S180-41
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Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelATTO 594
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SpeciesHuman, Humans, Mouses
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Alternative nameMouse Antibody toHuman EAAC1 monoclonal IgG1, clonality S180-41
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Alternative techniquemurine, antibodies
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Clone nameS180-41
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Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-41
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene namesolute carrier family 1 member 1
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Synonyms gene name
- solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1
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Synonyms
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Locus
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Discovery year1994-02-15
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Entrez gene record
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Pubmed identfication
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Classification
- Solute carriers
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3-41 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable 3-41
- immunoglobulin heavy variable 3-41 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable (VII)-41-1 (pseudogene)
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Synonyms gene name
- immunoglobulin lambda variable (VII)-41-1
- immunoglobulin lambda variable (VII)-41-1 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-05-15
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable 1-41 (pseudogene)
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Synonyms gene name
- immunoglobulin lambda variable 1-41
- immunoglobulin lambda variable 1-41 pseudogene
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 6D-41 (non-functional)
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Synonyms gene name
- immunoglobulin kappa variable 6D-41
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecoiled-coil domain containing 18
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Synonyms
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Locus
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Discovery year2005-06-24
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametripartite motif containing 71
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Synonyms gene name
- tripartite motif containing 71, E3 ubiquitin protein ligase
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Synonyms
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Locus
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Discovery year2006-03-30
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Entrez gene record
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RefSeq identity
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Classification
- Ring finger proteins
- Tripartite motif containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 41, pseudogene
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Synonyms gene
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Synonyms gene name
- RNA, U6 small nuclear 41
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Locus
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Discovery year2008-06-10
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Entrez gene record
Gene info
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Identity
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Gene
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Long gene namemethyltransferase like 25B
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Synonyms gene
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Synonyms gene name
- chromosome 1 open reading frame 66
- ribosomal RNA adenine dimethylase domain containing 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2005-05-17
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- 7BS orphan methyltransferases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
