Mouse Anti-Human Copper Transporting ATPase 2 Monoclonal IgG1, Clone S62-29
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Catalog numberSMC-399D-PCP
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PricePlease ask
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Size100 µg
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CloneS62-29
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ImmunogenSynthetic peptide amino acids 3-21 (cytoplasmic N-terminus) of human Copper-transporting ATPase2
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Antibody s full descriptionMouse Anti-Human ATP7B Monoclonal IgG1 Antibody, Clone: S62-29: PerCP
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameATP7B Antibody, ATPase Cu++ transporting beta polypeptide Antibody, ATPase Cu(2+) transporting beta polypeptide Antibody, Copper pump 2 Antibody, Copper transporting ATPase 2 Antibody, PWD Antibody, Toxic milk Antibody, tx Antibody, WC1 Antibody, WD Antibody, Wilson disease associated protein Antibody, WND Antibody, WND/140 kDa Antibody
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Raised inMouse
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Antibody s targetATP7B
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Primary research fieldsNeuroscience, Ion Channels, Cell Signaling
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF, IP
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~160kDa in rat brain membrane preparations.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-399 was sufficient for detection of Copper-transporting ATPase2 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat IgG:HRP as the secondary antibody.
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Antibody in cellCytoplasm , Mitochondrion , Golgi Apparatus , Trans-Golgi Network Membrane
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextThe copper efflux transporters ATP7A and ATP7B sequester intracellular copper into the vesicular secretory pathway for export from the cell. ATP7b is an important protein for copper transport and elimination of excess copper from the body. ATP7b transports metals in and out of cells using ATP. There are 3 known isoforms of the ATP7b gene; A is found in the liver, kidney, and brain, the shorter form B is found in brain tissue, and the third isoform, known as WND/140 KDA is found in mitochondria. Mutations in the ATP7b gene can cause Wilson's disease, an inherited disorder causing copper poisoning in the brain and liver, characterized by neurological symptoms and hepatic damage.
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Bibliography1. Tanzi R.E., et al. (1993) Nature Genetics. 5: 344-350. 2. Ghr/nlm.gov/gene/ATP7B
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Released date6-Jun-2012
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_000044.2
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Gene number540
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Protein numberB7ZLR4
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody, Clone S62-29 (SMC-399). Tissue: NIH 3T3 (Mouse Fibroblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody (SMC-399) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Copper Transporting ATPase 2 Antibody (D) Composite. Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa Copper Transporting ATPase 2 protein using Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody, Clone S62-29 (SMC-399). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain Membrane cell lysate. Load: 20 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody (SMC-399) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~160 kDa. Mouse Anti-Copper Transporting ATPase 2 Antibody [S62-29] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Mouse NIH 3T3 (Mouse Fibroblast cell line) (SMC-399) Mouse Anti-Copper Transporting ATPase 2 Antibody [S62-29] used in Western Blot (WB) on Rat Brain Membrane (SMC-399)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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Gene target
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Gene symbolSNORD114-29, SNORD115-29, SNORD116-29, IGLV3-29, IGHV3-29, IGKV2-29, IGKV2D-29, ERVK-29, ERVW-29, RNVU1-29
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Short nameMouse Anti- Copper Transporting ATPase 2 Monoclonal IgG1, Clone S62-29
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Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelPerCP
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SpeciesHuman, Humans, Mouses
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Alternative nameMouse Antibody toHuman Copper Transporting ATPase 2 monoclonal IgG1, clonality S62-29
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Alternative techniquemurine, antibodies
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Clone nameS62-29
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Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 114-29
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Synonyms
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Locus
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Discovery year2006-09-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-29
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 116-29
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable 3-29 (pseudogene)
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Synonyms gene name
- immunoglobulin lambda variable 3-29
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3-29 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable 3-29
- immunoglobulin heavy variable 3-29 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 2-29
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Synonyms gene name
- immunoglobulin kappa variable 2-29 (gene/pseudogene)
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 2D-29
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameendogenous retrovirus group K member 29
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Synonyms gene name
- endogenous retrovirus group K, member 29
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GenBank acession
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Locus
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Discovery year2014-01-03
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nameendogenous retrovirus group W member 29
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Synonyms gene name
- endogenous retrovirus group W, member 29
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GenBank acession
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Locus
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Discovery year2013-10-21
Gene info
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Identity
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Gene
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Long gene nameRNA, variant U1 small nuclear 29
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Locus
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Discovery year2019-07-26
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Classification
- Variant U1 small nuclear RNAs
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
