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Clone
PIN.1
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Immunogen
Human CD74 invariant chain synthetic peptide
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Antibody s full description
Mouse Anti-Human CD74 Monoclonal IgG Antibody, Clone: PIN.1: ATTO 390
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
DHLAG Antibody, HLA DR gamma Antibody, HLADG Antibody, p33 Antibody, p35 Antibody, Protein 41 Antibody, CD 74 antibody, CD74 antibody, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antibody, CD74 antigen antibody, CD74 molecule antibody, CD74 molecule, major histocompatibility complex, class II invariant chain antibody, CLIP antibody, DHLAG antibody, Gamma chain of class II antigens antibody, HG2A_HUMAN antibody, HLA class II histocompatibility antigen gamma chain antibody, HLA DR antigens associated invariant chain antibody, HLA DR gamma antibody, HLA-DR antigens-associated invariant chain antibody, HLA-DR-gamma antibody, HLADG antibody, HLADR antigens associated invariant chain antibody Ia antigen associated invariant chain antibody, Ia antigen-associated invariant chain antibody, Ia associated invariant chain antibody, Ia gamma antibody, Ii antibody, Invariant polypeptide of major histocompatibility complex class II antigen associated antibody, la-gamma antibody, Major histocompatibility complex class II invariant chain antibody, MHC HLA DR gamma chain antibody, MHC HLA-DR gamma chain antibody, p33 antibody, p35 antibody, Protein 41 antibody
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Raised in
Mouse
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Antibody s target
CD74
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Primary research fields
Immunology, Adaptive Immunity, B Cells, B Cell CD Markers
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Brandname
None
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Antibodies applications
WB, IHC, ICC/IF, IP, FCM, FACS
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Antibody s reactivity
Human, Mouse
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Antibody s dilutions
WB (1:1000), IHC (1:100), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
PBS pH7.2, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~33-35kDa protein doublet corresponding to the molecular mass of the p33 and p35 forms of human CD74.
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
1 µg/ml of SMC-116 was sufficient for detection of CD74 in 20 µg of PALA cell lysates by colorimetric immunolot analysis using goat anti-mouse IgG: AP as the secondary antibody.
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Antibody in cell
Cell Membrane , Endoplasmic Reticulum , Endoplasmic Reticulum Membrane , Golgi Apparatus , Endosome , Lysosome
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
CD74 is a non-polymorphic type II integral membrane protein. It has a short N-terminal cytoplasmic tail of 28 amino acids, followed by a single 24-aa transmembrane region and an approximately 150-aa lumenal domain (1). The CD74 chain is thought to function mainly as an MHC class II chaperone, which promotes ER exit of MHC class II molecules, directs them to endocytic compartments, prevents peptide binding in the ER, and contributes to peptide editing in the MHC class II compartment. Class II MHC and Ii expression was believed to be restricted to classical antigen-presenting cells (APC); however, during inflammation, other cell types, including mucosal epithelial cells, have also been reported to express class II MHC molecules (2). Experiments that investigate cell-surface CD74 are complicated by the fact that CD74 remains on the cell surface for a very short time. The surface half-life of CD74 was calculated to be fewer than 10 minutes (3). CD74 however has also recently been shown to have a role as an accessory-signaling molecule because of its high-affinity binding to the pro-inflammatory cytokine, macrophage migration-inhibitory factor (MIF) (3). The restricted expression of CD74 by normal tissues and its very rapid internalization make CD74 an attractive therapeutic target for both cancer and immunologic diseases (4).
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Bibliography
1. Becker-Hermann, S., Arie, G., Medvedovsky H, Kerem A, and Shachar I. (2005) Mol Bio Cell. 16(11):5061-9. 2. Barrera CA., et al (2005) J Histochem Cytochem 53 (12): 1481-9. 3. Starlets D., et al. (2006) Blood. 107 (12): 4807-4816. 4. Burton JD., et al. (2004). Clin Cancer Res. 10(19): 6606-11. 5. Denzin L.K., Hammond, C. and Cresswell, P. (1996) J. Exp. Med. 184: 2153-2165. 6. Denzin L.K., Robbins N.F., Carboy-Newcomb C. and Cresswell P. (1994) Immunity 1: 595-606.
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Released date
1-Sep-2010
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PubMed number
25035924, 19865478
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Tested applications
Western Blot, Western Blot
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Tested reactivity
Human, Human
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NCBI number
NP_001020329.1
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Gene number
972
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Protein number
P04233
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Antibody s datasheet
Contact our support service
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CD74 Monoclonal Antibody, Clone PIN 1.1 (SMC-116). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody (SMC-116) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus. Endosome. Lysosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-CD74 Antibody. (C) Composite. Immunohistochemistry analysis using Mouse Anti-CD74 Monoclonal Antibody, Clone PIN 1.1 (SMC-116). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody (SMC-116) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Beautiful basal to suprabasal staining in epidermis, dermis, hair follicles and muscle. Western Blot analysis of Human N87 cell lysates showing detection of CD74 protein using Mouse Anti-CD74 Monoclonal Antibody, Clone PIN 1.1 (SMC-116). Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody (SMC-116) at 1:1000. Lysates treated with macrophage inhibitory factor (MIF). Courtesy of: Victor E. Reyes, University of Texas Medical Branch, USA. Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CD74 Monoclonal Antibody, Clone PIN 1.1 (SMC-116). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody (SMC-116) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic Staining. Mouse Anti-CD74 Antibody [PIN 1.1] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SMC-116) Mouse Anti-CD74 Antibody [PIN 1.1] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-116) Mouse Anti-CD74 Antibody [PIN 1.1] used in Western Blot (WB) on Human N87 cell lysates (SMC-116) Mouse Anti-CD74 Antibody [PIN 1.1] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SMC-116)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin name
Mus musculus
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Gene target
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Gene symbol
CD74, DYNLL1
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Short name
Mouse Anti- CD74 Monoclonal IgG, Clone PIN 1
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Technique
anti-Human, anti-, Mouse, anti, IgG, antibody to, antibodies, antibodies against human proteins, IgGs, Monoclonals or monoclonal antibodies, mouses
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Host
mouse
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Isotype
IgG, IgG
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Label
ATTO 390
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Species
Human, Humans, Mouses
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Alternative name
Mouse Antibody toHuman CD74 molecule, major histocompatibility complex, class II invariant chain monoclonal Immunoglobulin G, clonality PIN.1
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Alternative technique
murine, antibodies, immunoglobulins
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Alternative to gene target
CD74 molecule, major histocompatibility complex, class II invariant chain, DHLAG and HLADG and Ia-GAMMA and II, CD74 and IDBG-53594 and ENSG00000019582 and 972, MHC class II protein binding, Cell surfaces, Cd74 and IDBG-150302 and ENSMUSG00000024610 and 16149, BT.46809 and IDBG-646288 and ENSBTAG00000015228 and 613384
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Clone name
PIN
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