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Clone
6F3
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Immunogen
A synthesized peptide derived from human CD68
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Antibody s full description
Mouse Anti-Human CD68 Monoclonal IgG1 Antibody, Clone: 6F3: ATTO 700
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
CD 68 Antibody, CD68 Antibody, CD68_HUMAN Antibody, Gp110 Antibody, LAMP4 Antibody, Macrosialin Antibody, SCARD1 Antibody, Scavenger receptor class D member 1 Antibody
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Raised in
Mouse
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Antibody s target
CD68
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Brandname
None
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Antibodies applications
IHC
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s dilutions
IHC (1:100); optimal dilutions for assays should be determined by the user.
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Antibody buffer for storage
PBS (without Mg2+ and Ca2+), 50% glycerol, pH 7.4, 150mM NaCl, 0.02% sodium azide
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Antibody s specificity
Detects endogenous CD68 proteins (~37KD ).
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
A 1:100 dilution of SMC-257 was sufficient for detection of CD68 in Human Tonsil Tissue using Goat Anti-Mouse IgG as the secondary antibody.
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Antibody in cell
Cell Membrane , Endosome membrane
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
CD68, which is homologous to the mouse antigen macrosialin, is an 85-115 kD member of the lysosomal-associated membrane protein (LAMP) family. It is a heavily glycosylated and predominantly found in cytoplasmi granules and in the cytoplasm of various non-hematopoietic tissues. Lamp proteins may have functions relating to cell-cell interaction or cell-ligand interaction.
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Bibliography
1. Ramprasad M.P., et al. (1996) Proc. Natl. Acad. Sci. USA 93:14833. 2. Smith M.J., et al. (1987) J. Cell. Sci. 87:113.
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Released date
06-Sep-2016
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
NP_001242.2
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Gene number
968
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Protein number
P34810
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Antibody s datasheet
Contact our support service
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Representative figure legend
Immunohistochemistry analysis using Mouse Anti-CD68 Monoclonal Antibody, Clone 6F3 (SMC-257). Tissue: Tonsil Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Mouse Anti-CD68 Monoclonal Antibody (SMC-257) at 1:100. Mouse Anti-CD68 Antibody [6F3] used in Immunohistochemistry (IHC) on Human Tonsil Tissue (SMC-257)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin name
Mus musculus
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Gene target
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Gene symbol
CD68
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Short name
Mouse Anti- CD68 Monoclonal IgG1 , Clone 6F3
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Technique
anti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
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Host
mouse
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Isotype
IgG1 , IgG1
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Label
ATTO 700
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Species
Humans, Mouses
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Alternative name
Mouse Antibody toHuman CD68 molecule monoclonal IgG1 , clonality 6F3
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Alternative technique
murine, antibodies
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Alternative to gene target
CD68 molecule, GP110 and LAMP4 and SCARD1, CD68 and IDBG-25923 and ENSG00000129226 and 968, Plasma membranes, Cd68 and IDBG-191443 and ENSMUSG00000018774 and 12514, CD68 and IDBG-635034 and ENSBTAG00000000133 and 504960
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Clone name
6F3
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