Mouse Anti-Human Ankyrin-B Monoclonal IgG2A, Clone S105-17
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Catalog numberSMC-414D-BI
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PricePlease ask
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Size100 µg
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CloneS105-17
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ImmunogenSynthetic peptide amino acids 203-496 of human Ankyrin-B
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Antibody s full descriptionMouse Anti-Human Ankyrin B Monoclonal IgG2A Antibody, Clone: S105-17: Biotin
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameANK-2 Antibody, ANK2 Antibody, Ankyrin 2 Antibody, Ankyrin-2 Antibody, Ankyrin nonerythroid Antibody, Brain ankyrin Antibody, non-erythroid ankyrin Antibody, Ankyrin-B Antibody
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Raised inMouse
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Antibody s targetAnkyrin B
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Primary research fieldsNeuroscience, Cell Structure, Membrane Markers, Cell Signaling, Scaffolds
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~>200kDa. No cross-reactivity against Ankyrin-G.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-414 was sufficient for detection of Ankyrin-B in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellCytoplasm , Cytoskeleton , Myofibril , Sarcomere , M line , Apical Cell Membrane , Cell Membrane
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextAnkyrins are a family of adaptor proteins that mediate the attachment of integral membrane proteins to the spectrin-actin based membrane skeleton (1). Ankyrins have binding sites for the beta subunit of spectrin and at least 12 families of integral membrane proteins. This linkage is required to maintain the integrity of the plasma membranes and to anchor specific ion channels, ion exchangers and ion transporters in the plasma membrane.
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Bibliography1. Bennett V., Baines A.J. (2001) Physiol. Rev. 81 (3): 1353–92.
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Released date3-Apr-2013
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_001120965.1
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Gene number287
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Protein numberQ01484
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin-B Monoclonal Antibody, Clone S105-17 (SMC-414). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin-B Monoclonal Antibody (SMC-414) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Ankyrin-B Antibody (D) Composite. Western Blot analysis of Rat brain membrane lysate showing detection of Ankyrin B protein using Mouse Anti-Ankyrin B Monoclonal Antibody, Clone S105-17 (SMC-414). Primary Antibody: Mouse Anti-Ankyrin B Monoclonal Antibody (SMC-414) at 1:1000. Mouse Anti-Ankyrin-B Antibody [S105-17] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-414) Mouse Anti-Ankyrin B Antibody [S105-17] used in Western Blot (WB) on Rat brain membrane lysate (SMC-414)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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Gene target
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Gene symbolPIRC95, PIRC91, PIRC90, PIRC93, PIRC92, SNORD115-17, IGHV3OR16-17, SNORD116-17
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Short nameMouse Anti- Ankyrin-B Monoclonal IgG2A, Clone S105-17
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Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG2A, IgG2a
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LabelBiotin
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SpeciesHuman, Humans, Mouses
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Alternative nameMouse Antibody toHuman Ankyrin-B monoclonal IgG2A, clonality S105-17
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Alternative techniquemurine, antibodies
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Clone nameS105-17
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Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 95
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 91
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 90
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 93
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 92
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-17
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3/OR16-17 (non-functional)
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Synonyms
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Locus
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Discovery year2020-06-26
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Entrez gene record
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Classification
- Immunoglobulin heavy (IGH) orphons
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 116-17
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
