Mouse Anti-Histone H4 (mono methyl K20) [5E10-D8]

• Catalog number
  GEN375123
• Price
  Please ask
• Size
  100ul

• Also known as
  Histone H4 (mono methyl K20)
• Other names
  histone H4; Histone H4; histone H4; histone 2, H4b; histone cluster 2, H4b; N/A
• Category
  Antibodies
• Subcategory
  Mnoclonal antibodies
• Gene name
  HIST2H4B; HIST1H4A; H4/o; H4/A; H4FA
• Gene name synonims
  HIST2H4B; HIST1H4A; H4/o; H4/A; H4FA
• Other gene names
  HIST2H4B; HIST1H4A; H4/o; H4/A; H4FA
• Clonality
  Monoclonal
• Immunoglobulin isotype
  IgG1
• Clone
  5E10-D8
• Host organism
  Mouse (Mus musculus)
• Species reactivity
  Human (Homo sapiens). Predicted to work with: Xenopus laevis, Arabidopsis thaliana, Fruit fly (Drosophila melanogaster), all Mammals; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
• Specificity and cross reactivity
  Specific for K20 mono-methylated histone H4; no cross-reactivity with K20 tri-, di-, or unmethylated histone H4; no cross-reactivity with histone H3.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
• Purification method
  Protein G
• Form Appearance
  Purified antibody (from supernatant) containing PBS + 0.1% sodium azide
• Concentration
  1 miligram per 1 mililiter
• Storage and shipping
  Lyophilized antibodies may be transported at ambient temperature and stored for short terms at +4 degrees Celsius and at -20 for long term storage. Antibodies in liquid form can be shipped and stored for short terms at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol has to added and the vial must be stored at -20°C.
• Tested applications
  Dot Blot (DB), Western Blot (WB) against endogenous protein, ELISA (EIA), Chromatin Immunoprecipitation (ChIP)
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. A methyl group is an alkyl derived from methane, containing one carbon atom bonded to three hydrogen atoms — CH3. In formulas, the group is often abbreviated Me. Such hydrocarbon groups occur in many organic compounds. It is a very stable group in most molecules. While the methyl group is usually part of a larger molecule, it can be found on its own in any of three forms anion, cation or radical. The anion has eight valence electrons, the radical seven and the cation six. All three forms are highly reactive and rarely observed,
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  Histone   mono   methyl   K20   [5E10-D8]  
• Gene symbol
  KRT20, UBA7
• Short name
  Mouse Anti-Histone H4 (mono methyl K20) [5E10-D8]
• Technique
  anti-, Mouse, anti, antibody to, antibodies, mouses
• Host
  mouse
• Species
  Mouse, Mouses
• Alternative name
  Anti-Mouse Anti-Histone H4 (mono methyl K20) [5E10-D8]
• Alternative technique
  murine, antibodies

Gene info

• Identity
  HGNC:20412
• Gene
  KRT20
• Long gene name
  keratin 20
• Synonyms gene name
  • keratin 20, type I
• Synonyms
  • CK20
  • K20
  • MGC35423
• GenBank acession
  • BC031559
• Locus
  17q21.2
• Discovery year
  2003-02-04
• Entrez gene record
  54474
• Pubmed identfication
  • 8359595
  • 12515621
  • 16831889
• Classification
  • Keratins, type I
• VEGA ID
  OTTHUMG00000133366

Gene info

• Identity
  HGNC:12471
• Gene
  UBA7
• Long gene name
  ubiquitin like modifier activating enzyme 7
• Synonyms gene
  • UBE1L
• Synonyms gene name
  • ubiquitin-activating enzyme E1-like
  • ubiquitin-like modifier activating enzyme 7
• Synonyms
  • D8
  • UBE2
  • UBA1B
• Synonyms name
  • UBA1, ubiquitin-activating enzyme E1 homolog B (yeast)
  • UBA7, ubiquitin-activating enzyme E1
• GenBank acession
  • BC006378
• Locus
  3p21.31
• Discovery year
  1993-11-24
• Entrez gene record
  7318
• Pubmed identfication
  • 8327486
• RefSeq identity
  • NM_003335
• Classification
  • Ubiquitin like modifier activating enzymes
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000158267

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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