c-Myc (human) Recombinant monoclonal antibody (9E10) (Mouse Fab fragment kappa) (His-tag)
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Catalog number
MBS566945
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Price
Please ask
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Size
0.1 mg
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Products_type
Antibody
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Products_short_name
[c-Myc]
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Other_names
[myc proto-oncogene protein; Myc proto-oncogene protein; myc proto-oncogene protein; proto-oncogene c-Myc; transcription factor p64; class E basic helix-loop-helix protein 39; avian myelocytomatosis viral oncogene homolog; v-myc avian myelocytomatosis viral oncogene homolog; myc-related translation/localization; v-myc myelocytomatosis viral oncogene homolog (avian); Class E basic helix-loop-helix protein 39; bHLHe39; Proto-oncogene c-Myc; Transcription factor p64]
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Products_gene_name
[c-Myc]
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Other_gene_names
[MYC; MYC; MRTL; c-Myc; bHLHe39; BHLHE39; bHLHe39]
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Clonality
Monoclonal
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Clone
[9E10]
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Reactivity
Human
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Specificity
Recognizes epitope sequence EQKLISEEDL, located in aa 410-419 of human c-myc protein
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Purity
Immobilized metal affinity chromatography purified
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Form
Liquid. In PBS containing 0.02% Proclin 300
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Storage_stability
Stable at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C. Avoid freeze/thaw cycles Shipping: Shipped on Blue Ice
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Tested_application
Immunofluorescence (IF), Immunohistochemistry (IHC) Frozen, Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB)
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Properties
If you buy Antibodies supplied by MyBioSource they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human Fab Of Normal IgG Chromatographically purified Fab fragment of human IgG. Purified human Fab may be used as a reference antigen, calibrator, coating protein and blocking agent, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
histidine
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Source
Recombinants or rec. proteins
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Group
recombinants
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French translation
anticorps
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Gene target
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Short name
c-Myc ( ) Recombinant monoclonal antibody (9E10) (Mouse Fab kappa) (His-tag)
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Technique
Recombinant, Antibody, Mouse, Fab, antibodies against human proteins, antibodies for, fabs, Monoclonals or monoclonal antibodies, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. MyBioSource advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Host
Mouse
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Isotype
Mouse IgG1, Fab fragment, Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common.
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Label
His
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Species
Mouse, Humans, Mouses
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Alternative name
c-v-myc myelocytomatosis viral oncogene homolog (avian) (H. sapiens) Rec. monoclonal (antibody to-) (9E10) (Mouse Fab fragment kappa) (histidine-detection labelled)
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Alternative technique
rec, antibodies, murine, seconairy
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Alternative to gene target
v-myc myelocytomatosis viral oncogene homolog (avian), bHLHe39 and c-Myc and MRTL and MYCC, MYC and IDBG-35442 and ENSG00000136997 and 4609, E-box binding, nuclei, Bmyc and IDBG-147082 and ENSMUSG00000049086 and 107771, MYC and IDBG-644147 and ENSBTAG00000008409 and 511077
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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