-
Clone
HA.C5
-
Immunogen
A synthetic peptide from influenza hemagglutinin epitope (YPYDVPDYA) coupled to KLH.
-
Antibody s full description
Mouse Anti-Human HA tag Monoclonal IgG3 Antibody, Clone: HA.C5: PE/ATTO 594
-
Antibody s category
Monoclonal Antibodies
-
Antibody s other name
HA epitope tag antibody, HA1 antibody, HA2 antibody, hemagglutinin antibody, Hemagglutinin HA1 chain antibody, Hemagglutinin HA2 chain antibody
-
Raised in
Mouse
-
Antibody s target
HA tag
-
Brandname
None
-
Antibodies applications
WB, ICC/IF, IP
-
Antibody s reactivity
Species Independent
-
Antibody s dilutions
WB (1:1000), ICC/IF (1:400), IP (1:400); optimal dilutions for assays should be determined by the user.
-
Antibody buffer for storage
PBS, pH 7.4, 50% glyercol, 0.05% sodium azide
-
Antibody s specificity
HA-tag monoclonal antibody. Recognizes HA-tagged proteins overexpressed in cells, including both amino- or carboxy-termini of targeted proteins in transfected mammalian cells.
-
Storage recommendations
-20ºC
-
Shipping recommendations
Blue Ice or 4ºC
-
Antibody certificate of analysis
A 1:1000 dilution of SMC-260 was sufficient for detection of HA Tag in 10 µg of Human Hek293 cell lysates by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
-
Antibody in cell
See included datasheet or contact our support service
-
Tissue specificity
See included datasheet or contact our support service
-
Scientific context
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 and has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
-
Bibliography
1. Schembri, L. et al. (2007) Nature Methods 4 (2): 107–108.
-
Released date
06-Sep-2016
-
Tested applications
To be tested
-
Tested reactivity
To be tested
-
NCBI number
Refer to NCBI
-
Gene number
Refer to GenBank
-
Protein number
Refer to Swiss-Prot
-
Antibody s datasheet
Contact our support service
-
Representative figure link
-
Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HA Tag Monoclonal Antibody, Clone HA.C5 (SMC-260). Primary Antibody: Mouse Anti-HA Tag Monoclonal Antibody (SMC-260). Secondary Antibody: APC Goat Anti-Mouse (red). Western Blot analysis of Human Hek293 cell lysates showing detection of HA Tag protein using Mouse Anti-HA Tag Monoclonal Antibody, Clone HA.C5 (SMC-260). Lane 1: Human 293T cells (Untransfected Control). Lane 2: Human 293T cells transfected with HA-Tag Vector.. Primary Antibody: Mouse Anti-HA Tag Monoclonal Antibody (SMC-260) at 1:1000. Mouse Anti-HA Tag Antibody [HA.C5] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on (SMC-260) Mouse Anti-HA Tag Antibody [HA.C5] used in Western Blot (WB) on Human Hek293 cell lysates (SMC-260)
-
Warning information
Non-hazardous
-
Country of production
Canada
-
Total weight kg
1.4
-
Net weight g
0.1
-
Stock availability
In Stock
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus
-
Gene target
-
Short name
Mouse Anti-HA tag Monoclonal IgG3, Clone HA C5
-
Technique
anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses, Tags
-
Host
mouse
-
Isotype
IgG3, IgG3
-
Label
PE/ATTO 594
-
Species
Mouse, Mouses
-
Alternative name
Mouse Antibody toHA detection labelled monoclonal IgG3, clonality HA.complement component 5
-
Alternative technique
murine, antibodies
-
Alternative to gene target
complement component 5, C5a and C5b and C5D and CPAMD4 and ECLZB, C5 and IDBG-83167 and ENSG00000106804 and 727, C5a anaphylatoxin chemotactic receptor binding, Extracellular, Hc and IDBG-163835 and ENSMUSG00000026874 and 15139, BT.24055 and IDBG-638834 and ENSBTAG00000012210 and 512045
-
Clone name
HA
-
