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Category
Primary Antibodies
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Long description
Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo- orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl- sphingomyelin was the most effective, followed by palmitoyl- sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group (By similarity).
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Antibody come from
Recombinant human SLP-76 fusion protein
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Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
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Clone
NK-1
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Antigen antibody binding interaction
Mouse anti CD57 Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western blot, Immunoprecipitation, Immunofluorescence, Immunohistochemistry
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Antibody s suited for
Applications: Western Blot: This antibody can be used at 1ug/ml to visualize 1 x 10^5 Jurkat cells per lane. Immunoprecipitation: This antibody can be used at a range from 1 to 5 ug per 1x10^6 Jurkat cells._x000B_Optimal concentration should be evaluated by serial dilutions.
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Storage
-80ºC
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Relevant references
1.Jackman, K. J., Motto, D. G., Sun, Q.,Tanemoto, M., Turk, C.W., Peltz, G. A., Koretzky, G. A., Findell, P.R. (1995) J. Biol. Chem. 270, 7029-7032_x000B__x000B_2. Wardenburg,J.B., Fu,C., Jackmann,J.K., Flotow, H., Wilkinson,S.E., Williams,D.H., Johnson,R., Kong, G., Chan, A.C., Findel, P.R. (1996) J. Biol. Chem. 271, 19641-19644
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus