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Category
Primary Antibodies
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Long description
Single-chain membrane glycoprotein that forms a non-covalent complex with GPIb. (MW 23kDa) Reactivity with resting and activated platelets, weakly on monocytes, megakaryocytes and attachment site for the platelet plasma membrane to the submembrane cytoskeleton. GPIb/IX complex, functions as the receptor for ristocetin-induced binding of von Willebrand factor and as the von Willebrand factor-depend adhesion receptor.
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Antibody come from
n/a
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Other description
Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein. Protein A/G Chromatography
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Clone
ESS
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Antigen antibody binding interaction
Mouse anti CD42a antibody conjugated to FITC Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Research interest
CD Marker
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Application
Flow Cytometry
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Antibody s suited for
Consult the appropriate Exalpha Negative Control factsheet to determine the amount of antibody to be used as a control for Platelets. Collect blood aseptically by venipuncture into an ACD or EDTA blood collection tube. Important: Within 5 minutes of blood collection, fix blood sample by placing 100 µl of blood in test tube containing 1 ml of cold (4¼C) 1X PBS with 1% paraformaldehyde. Mix by vortexing. Centrifuge the fixed blood at 1200 x g for 5 minutes at room temperature (RT) 20¼C. Aspirate the supernatant, leave pellet. Prior to staining, wash the fixed blood pellet 2X with 1 ml 1X PBS + 0.1% Azide at RT. Centrifuge the fixed blood at 1200 x g for 5 minutes at room temperature (RT) 20¼C. Aspirate the supernatant, leave pellet. Resuspend the pellet in 1 ml of 1X PBS at RT. To a clean labeled test tube add 10 µl of Exalpha monoclonal antibody. Carefully add 50 _l of the fixed blood suspension to the bottom of the test tube. Vortex and incubate at room temperature for at least 15 minutes and analyzed within 3 hours. Please note: Using this procedure will yield twenty samples for platelet analysis. Select logarithmic (Log) amplification for both Forward (FSC) and Side (SSC) scatters, while collecting data for platelets. See instrument manufacturerÕs instructions for Immunofluorescence analysis with a flow cytometer or microscope.
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Storage
4ºC
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Relevant references
1. Detection of platelet activation with monoclonal antibodies and flow cytometry. Fijnheer R., Modderman P.W., Veldman H., et al. Transfusion 1990:30:20-25 _x000B__x000B_2. Glycoprotein Ib and glycoprotein IX are fully complexed in the intact platelet membrane Du X, Beutler L., Ruan C., Castaldi P.A., and Berndt M.C., Blood 1987:69:1524-1527 _x000B__x000B_3. Structure of the glycoprotein Ib-IX complex from platelet membranes Fox JEB, Aggerbeck L.P., Berndt M.C., J. Biol. Chem. 1988:263:4882-4890 _x000B__x000B_4. Leucocyte Typing IV: White Cell Differentiation Antigens Nieuwenhuis H.K. Report on functional studies. In: Knapp W., Dorken B., Gilks W.R., et al, eds. Oxford: Oxford University Press: 1989:1002-1003.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This nordc Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. nordc FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps
