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Category
Primary Antibodies
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Long description
CXCR1 and CXCR2, previously known as IL-8RA, or type I IL-8 receptor, and IL-8RB, or type II IL-8 receptor, respectively, have been shown to share approximately 77% amino acid sequence identity. IL-8 binds to both receptors with high affinity and induces rapid elevation of cytosolic Ca2+ levels. While IL-8 and Granulocyte Chemotactic Protein 2 (GCP2) have been shown to exert their biological activity by binding to both receptors, GRO , GRO , GRO , NAP-2 and ENA-78 bind only to CXCR2. CXCR1 and CXCR2 are expressed on neutrophils and mast cells, but not on B or T lymphocytes. In cells transfected with either CXCR1 or CXCR2, clone 501 showed no cross-reactivity with CXCR2.
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Antibody come from
Purified recombinant mouse IL-10.
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Other description
Provided as solution in phosphate buffered saline, pH 7.3, with 1.0 mg/ml BSA and 0.05% sodium azide. Protein A/G Chromatography
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Clone
501
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Antigen antibody binding interaction
Mouse anti CXCR1 Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
ELISA, Western Blot, Immunohistochemistry, immunocytochemistry,Flow Cytometry
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Antibody s suited for
This antibody is suitable for use in ELISA as a coating antibody. This clone has also been found to be neutralizing based on its ability to inhibit mouse macrophage TNF- production and IL-4 and IL-10 induced MC-9 (mast cell line) proliferation. Optimal concentration should be evaluated by serial dilutions. Immediately prior to use, dilute this preparation to a final concentration of 1-5 µg/mL, and coat each well of a microtiter plate with 100 µL. For neutralization studies, use a starting dilution of 10-20 µg/mL.
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Storage
-20ºC
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Relevant references
1. Hunter, C. A. et al. (1995) IL-1 beta is required for IL-12 to induce production of IFN gamma by NK cells. A role for IL-1 beta in the T cell-independent mechanism of resistance against intracellular pathogens. J. Immunol. 155(9):4347-54._x000B__x000B_2. Rohrer, J. W. and J. H. Coggin (1995) CD8 T cell clones inhibit antitumor T cell function by secreting IL-10. J. Immunol. 155(12):5719-27._x000B__x000B_3. Shnyra, A. et al. (1998) Reprogramming of lipopolysaccharide-primed macrophages is controlled by a counterbalanced production of IL-10 and IL-12. J. Immunol. 160(8):3729-36. _x000B__x000B_4. Schwarz, A., S. Beissert, K. Grosse-Heitmeyer, M.Gunzer, J.A. Bluestone, S. Grabbe, T. Schwarz (2000) Evidence for functional relevance of CTLA-4 in ultraviolet-radiation-induced tolerance. J. Immunol. 165(4):1824-1831 (cites the use of this antibody for use in neutralization in vivo)._x000B_
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
