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Category
Primary Antibodies
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Long description
The chance of cure and choice of therapy for squamous carcinomas of the head and neck depend largely on the extent of disease, as assessed clinically by inspection,palpation and radiography. Since treatment failures are in part due to clinically undetected metastasis at the time of treatment, there is a need for more specific methods of tumor detection. Attempts to develop antibodies recognizing tumour-associated antigens have, in the case of carcinomas, resulted in the isolation of a large number of antibodies reactive with high-molecular-weight glycoproteins. Monoclonal antibodies (mAbs) 175F4 (IgG1) and 175F11 (IgG2a), originally raised anti the Human mammary carcinoma cell line (ZR-75-1), react with a carcinoma-associated antigen in both adenocarcinomas and squamous cell carcinomas of different origins. Immunohistochemically, the mAbs exhibited reactivity with 42 out of 43 squamous cell carcinomas of the head and neck. Normal squamous epithelia are also reactive with the antibodies in the basal and suprabasal cell layer.
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Antibody come from
175F4 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with EDTA treated Human mammary carcinoma cell line (ZR-75-1).
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Other description
Each vial contains 1ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide.
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Clone
175F4
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Antigen antibody binding interaction
Mouse anti Carcinoma ass. Antigen Antibody
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Antibody is raised in
Mouse (Balb/c)
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
175F4, which, originally, has been raised anti the Human mammary carcinoma cell line (ZR-75-1), react with a carcinoma-associated antigen in both adenocarcinomas and squamous cell carcinomas of different origins.
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Research interest
Cancer
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Application
Immunohistochemistry (frozen)
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Antibody s suited for
175F4 is useful for immunohistochemistry on frozen tissue. Optimal antibody dilution should be determined by titration.
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Storage
Store at 4°C, or in small aliquots at –20°C.
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Relevant references
Balm AJ, Hageman PC, Mulder CL, Hilkens J. Carcinoma-associated monoclonal antibodies in head and neck carcinoma. Immunohistochemistry and biodistribution of monoclonal antibodies 175F4 and 175F11. Eur Arch Otorhinolaryngol. 1992;249(5):237-42. Joyce Taylor-Papadimitriou. Report On The First International Workshop On Carcinoma-Associated Mucins. Int. J. Cancer: 49, 1-5 (1991).
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antigens are peptides or recombinant or native dependent on the production method.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
