Mouse Anti- Alginate Monoclonal IgG1 Kappa, Clone 3G4-1F5
-
Catalog numberSMC-209D-A488
-
PricePlease ask
-
Size100 µg
-
-
Clone3G4-1F5
-
ImmunogenSodium Alginate conjugated to KLH
-
Antibody s full descriptionMouse Anti- Alginate Monoclonal IgG1 Kappa Antibody, Clone: 3G4-1F5: ATTO 488
-
Antibody s categoryMonoclonal Antibodies
-
Antibody s other nameAlginic Acid Antibody, Algin Antibody, Sodium Alginate Antibody
-
Raised inMouse
-
Antibody s targetAlginate
-
Primary research fieldsCell Signaling, Post-translational Modifications
-
BrandnameNone
-
Antibodies applicationsWB, IHC
-
Antibody s reactivitySpecies Independent
-
Antibody s dilutionsWB (1:500); optimal dilutions for assays should be determined by the user.
-
PurityProtein G Purified
-
Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
-
Antibody s concentration1 mg/ml
-
Antibody s specificityBinds selectively to a BSA-conjugated alginate, but not to unconjugated BSA.
-
Storage recommendations-20ºC
-
Shipping recommendationsBlue Ice or 4ºC
-
Antibody certificate of analysis1 µg/ml of SMC-209 was sufficient for detection of 150 ng of alginate-conjugated BSA by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
-
Antibody in cellSee included datasheet or contact our support service
-
Tissue specificitySee included datasheet or contact our support service
-
Scientific contextSodium alginate is used in biological experiments for the immobilization of cells and encapsulation due to its biocompatibility and simple gelation with divalent cations such as Ca2+. Studies suggest that preparation of alginate microspheres will sustain protein delivery within tissue scaffolds (1), as well as in many other cell types (2).
-
Bibliography1. Zhai P., Chen X.B., and Schreyer D.J. (2013) Biofabrication. 5(1): 015009. 2. Selimoglu S.M., Ayyildiz-Tamis D., Gurhan I.D., and Elibol M. (2012) J Biosci Bioeng. 113(2):233-238.
-
Released date24-Jun-2014
-
Tested applicationsTo be tested
-
Tested reactivityTo be tested
-
NCBI numberRefer to NCBI
-
Gene numberRefer to GenBank
-
Protein numberRefer to Swiss-Prot
-
Antibody s datasheetContact our support service
-
Representative figure legendWestern Blot analysis of ALL BSA-Alginate Conjugate showing detection of ~250 kDa Alginate protein using Mouse Anti-Alginate Monoclonal Antibody, Clone 3G4-1F5 (SMC-209). Lane 1: MW ladder. Lane 2: 0.625ug BSA:Alginate. Load: 0.625 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-Alginate Monoclonal Antibody (SMC-209) at 1:500 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:100 for 1 hour at RT. Color Development: TMB solution for 2 min at RT. Predicted/Observed Size: ~250 kDa. Mouse Anti-Alginate Antibody [3G4-1F5] used in Western Blot (WB) on ALL BSA-Alginate Conjugate (SMC-209)
-
Warning informationNon-hazardous
-
Country of productionCanada
-
Total weight kg1.4
-
Net weight g0.1
-
Stock availabilityIn Stock
-
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
-
AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
-
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin nameMus musculus
-
Gene target
-
Gene symbolIL36RN
-
Short nameMouse Anti- Alginate Monoclonal IgG1 Kappa, Clone 3G4-1F5
-
TechniqueMouse, anti, antibody to, Monoclonals or monoclonal antibodies, mouses
-
Hostmouse
-
IsotypeIgG1 Kappa, IgG1
-
LabelATTO 488
-
SpeciesMouse, Mouses
-
Alternative nameMouse antibody to- Alginate monoclonal IgG1 Kappa, clonality 3G4-1F5
-
Alternative techniquemurine, antibodies
-
Clone name3G4-1F5
-
Gene info
-
Identity
-
Gene
-
Long gene nameinterleukin 36 receptor antagonist
-
Synonyms gene
-
Synonyms gene name
- interleukin 1 family, member 5 (delta)
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year2002-08-02
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Interleukins
-
VEGA ID
-
Locus Specific Databases
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data